Sos2 (NM_001135561) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sos1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208299 representing NM_001135561 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCAGGCGCCGCAGCCCTACGAGTTCTTCAGCGAAGAGAACAGCCCGAAATGGCGGGGACTGTTGG TCCCGGCCCTGCGGAAGGTTCAGGAGCAAGTGCACCCCACCCTGTCGGCTAACGAAGAGTCTCTCTATTA CATCGAAGAACTCATCTTTCAACTGCTCAATAAGCTGTGCCTGGCTCAACCGAGGACTGTTCAAGATGTT GAGGAACGAGTTCAAAAGACTTTTCCTCATCCTATTGATAAATGGGCAATTGCTGATGCACAGTCTGCCA TAGAGAAACGAAAACGAAGAAATCCTCTCTTACTACCTGTGGACAAAATCCATCCTTCCTTGAAGGAAGT TTTGGGGTATAAAGTGGACTACCATGTATCCCTCTACATTGTGGCTGTATTGGAGTATATCTCAGCAGAT ATTTTGAAATTGGCTGGTAATTATGTTTTTAATATCCGGCATTATGAAATATCTCAGCAAGACATTAAAG TGTCAATGTGTGCAGATAAGGTTTTGATGGACATGTTTGACCAGGATGACGATATAGGCTTGGTTTCTCT CTGTGAAGAGGAACCTTGTTCTTCTGGTGAGCTAAACTATTATGACCTTGTTAGAACTGAAATCGCAGAA GAAAGACAGTATCTACGGGAACTGAATATGATCATTAAAGTATTCCGGGAAGCCTTTCTTTCAGACAAAA AGTTGTTCAAGTCTTCTGAAATTGAAAAGATTTTCAGTAACATTTCAGATATACATGAACTGACTGTGAA ACTTTTAGGTTTGATTGAAGACACAGTAGAAATGACAGATGAAAGCAGTCCTCACCCGCTAGCTGGTAGC TGCTTTGAAGATTTAGCAGAGGAGCAGGCTTTTGACCCCTATGAAACGTTATCACAGGACATTCTTGCTC CAGAGTTTAATGACCACTTCAGCAAGTTGATGGCCAGACCTGCAGTTGCTCTGCACTTTCAGTCCATTGC TGACGGCTTTAAGGAGGCTGTTCGATATGTCCTTCCGCGCCTCATGCTGGTGCCGGTGTATCATTGTTGG CATTACTTTGAATTATTAAAGCAGTTGAAAGCATGTAGCGAAGAACAGGAAGACCGAGAGTGTTTGAATC AGGCTATCACTGCCCTCATGAACCTCCAAGGCAGCATGGACCGGATTTACAAGCAGCACTCGCCAAGGCG CCGGCCAGGGGATCCTGTTTGCCTTTTTTACAATCGTCAATTAAGAAGCAAGCACCTGGCTATCAAAAAA ATGAATGAAATTCAGAAAAACATTGATGGGTGGGAAGGCAAAGATATTGGACAGTGTTGTAATGAGTTCA TAATGGAAGGACCATTGACCAGAATTGGTGCTAAGCATGAAAGGCATATTTTCCTCTTCGATGGCTTAAT GATCAGCTGTAAACCCAATCATTGCCAGACTCGACTTCCAGGGTATAGTAGTGCAGAATACAGGTTAAAA GAGAAGTTTGTCATGAGGAAAATTCAAATCTGTGATAAAGAAGACGCTTGTGAATACAAACATGCTTTTG AATTAGTATCCAAAGATGAAAACAGTGTAATATTTGCTGCCAAGTCAGCTGAAGAGAAAAACAACTGGAT GGCAGCCCTTATCTCCCTTCACTATCGGAGCACTCTAGATAGAATGCTGGACTCTGTGTTACTGAAAGAG GAGAACGAGCAGCCCCTGCGGCTACCGCGGCCAGACGTGTACCGCTTCGTGGTGACAGACTCAGAGGAGA ACATAGTTTTTGAAGACAACTTGCAAAGCAGAAGTGGAATCCCCATTATTAAAGGAGGCACCGTGGTGAA ACTGATCGAAAGGCTAACATATCACATGTATGCAGATCCCAATTTTGTTCGTACTTTCCTTACTACGTAT CGTTCATTTTGTAAGCCACAGGAATTGCTAAGCTTGCTGATAGAACGGTTTGAAATTCCAGATCCAGAGC CTACTGAGGCCGACAAACTGGCGCTAGAGAAAGGCGAGCAGCCAATCAGCGCAGATCTGAAAAGATTCCG CAAGGAATATGTCCAACCCGTGCAACTTAGGGTCTTGAATGTCTTTCGCCACTGGGTTGAGCATCATTTT TATGACTTTGAAAGAGACTTGGAATTGCTTGAAAGACTGGAAGCCTTCATTTCAAGTGTAAGAGGGAAAG CCATGAAGAAGTGGGTAGAATCCATTGCTAAAATCATCAAGAGGAAGAAGCAAGCTCAGGCCAATGGAGT AAGCCATAATATCACCTTTGAAAGTCCGCCTCCACCGGTTGAATGGCACATAAGTAGGGCGGGACAGTCT GAAACCTTTGACCTTATGACACTTCATCCCATAGAGATTGCACGGCAGTTAACACTCTTGGAATCTGATC TCTACAGGAGAGTCCAGCCTTCTGAACTTGTAGGGAGTGTCTGGACCAAAGAAGATAAAGAAATAAATTC TCCAAATCTATTAAAAATGATTCGCCATACAACAAACCTCACTTTGTGGTTTGAAAAATGCATTGTGGAA GCAGAAAACTTTGAAGAACGGGTGGCAGTGCTCAGCAGAATAGTAGAAATTCTGCAAGTGTTTCAAGATC TGAATAATTTCAATGGCGTGTTGGAGATAGTCAGTGCAGTCAATTCAGTGTCAGTGTACAGACTAGACCA CACATTTGAGGCATTGCAGGAAAGGAAGCGGAGAGTTTTGGATGACGCTGTGGAATTAAGTCAGGATCAC TTTAAAAAATACCTAGTAAAACTTAAATCAATCAATCCACCTTGTGTGCCTTTTTTTGGAATATATTTAA CAAATATTCTGAAGACTGAAGAAGGGAACAGCGACTTTCTAAAGAGGAAAGGGAAAGATTTGATCAATTT CAGTAAGAGGAGAAAAGTGGCTGAAATAACCGGAGAGATTCAGCAGTATCAGAACCAACCATACTGCTTA CGGACAGAACCAGAGATGAGGAGATTCTTTGAAAACCTCAATCCCATGGGACTTTTATCTGAAAAAGAGT TTACAGATTATTTGTTCAACAAATCTCATGAAATTGAACCCCGAAACTGCAAACAACCACCTCGATTTCC TAGGAAGTCAACCTTTTCCTTAAAATCTCCTGGAATAAGGCCCAATGCCAGCCGCCATGGCTCTACCTCA GGCACGTTCCGAGGTCACCCAACCCCTCTGGAAAGGGAGCCTTATAAGATAAGCTTTAGCAGGATCGCCG AGACGGAGCTAGAATCCACGGTGTCAGCACCGACATCTCCCAACACCCCATCCACCCCACCTGTGTCTGC TTCTTCAGACCACAGCGTGTTTCTAGATGTGGACCTCAATAGCTCCTGTGGCAGCAATACCATCTTTGCT CCAGTCCTCTTGCCACACTCAAAGTCTTTCTTCAGCTCATGTGGAAGTCTACACAAACTGAGTGAAGAGC CACTAGTTCCTCCTCCGCTTCCCCCTCGGAAAAAGTTTGATCACGATGCTCCGAACTCCAAGGGAGCTAT GAAATCGGATGATGACCCTCCTGCTATTCCACCAAGACAACCCCCTCCTCCAAAGGTAAAGCCAAGAGCT CCTGTCCTCACGGGTACATTTGAAGGGCCTGTGCCCAGTCCACCTCCACCTCCTCCCAGAGACCCTCTTC CTGATACTCCTCCACCAGTTCCTCTTCGGCCTCCGGAACACTTTATAAACTGTCCGTTTAATCTCCAGCC GCCTCCGCTGGGACATCCTCACAGAGACCCAGACTGGCTCAGAGACGTTAGCACATGCCCTAATTCACCA AGCACTCCTCCCCCTACGCCCTCGCCACGGATTCCACGCAGATGCCATTTGCCCAGCCCCAGTCACAACA ATCTTGCTCACCCTCCAGCTCCTCCCGTTCCACCAAGGCAGAATTCAAGCCCTCACCTACCAAAACTGCC ACCAAAGACTTACAAGCGGGAGCTTTCACACCCGCCATTGTACAGACTACCTTTGTTTGAAAATGCAGAA ACTCCTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135561
Insert Size	4002 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135561.1, NP_001129033.1
RefSeq Size	5365 bp
RefSeq ORF	4002 bp
Locus ID	85384
Gene Summary	mRNA expression increases after chronic clozapine treatment in the prefrontal cortex; may play a role in synaptic function [RGD, Feb 2006]

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