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Nup85 (NM_001025401) Rat Untagged Clone
CAT＃: RN208234
Nup85 (untagged ORF) - Rat nucleoporin 85kDa (Nup85), (10 ug)
CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Pcnt1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN208234 representing NM_001025401
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCTCGACTGCGAGCCCGCAGTAACTTGGATTCCAGGTGTGAATTCCAAGAAGAAGCAAATGT GTTTTGACTGGGGCCCAGGGGAGATGCTCCTATGTGAAACTTCCTTCAACAAAACAGACAAATCAGAGAA GGTCCCGAGCTGCCCCTTTATTTATATCATAAGGAAAGATGTCGATGTTTACTCTCAAATTTTGAGAAAA CTCTTCAACGAATCCCATGGAATCTTCGTTGGCCTGCAAAGACTTGAGGAAGAACTGTCCGGGAAATCCA GGAAAGCTCAATTGGTTCGAGTGAGTAAAAATTACCGGTCAGTTATACGTGCCTGTATGGAAGAAATGCA CCAGGTTGCAATTGCTGCTAAAGACCCAGCCAGTGGCCGGCAGTTCAGCAGTCAGGTCTCCATTTTGTCA GCCATGGAGCTTATTTGGAACCTGTGCGAGATTCTTTTCATTGAAGTAGCTCCAGCTGGACCTCTCCTCC TCCATCTTCTTGACTGGGTCCGACTGCACGTGTGCGAGGTGGACAGTTTGTCAGCAGATGTCCTGGGCAG TGACCACCCAAGCAAGCATGAGAGCTTCTGGAACTTGGTGACCGTCTTGGTGCTTCAGGGCCGGCTGGAT GAGGCCCGACAAATGCTGTCCAAAGAAGCTGATGCCAGCCCCTCTTCTGCAGGCATGTGCCGCGTCCTTG GGGATCTGATGAGGACAATGCCCATTCTCAGTCCTGGCAACACTCAGACGCTGACAGAGTTGGAGCTGAA GTGGCAGCACTGGCGTGAGGAGTGTGAGCGACACTTGCAAGACAACACATTTGCAGCCAACCCCCATCTG GAGTCTCTCTGCAAGATCATGCTGGGGGATGAGGCCACCTTGTTGGAGCAGAAGGAGCTTATGAGCAACT GGTACCATTTCTTAGTGACTCGGCTGCTGTATTCCAACCCCACAGTGAAGCCCACTGACCTGCACTTGTA CGCCCAGTCCAGCCTGGACATGTTTCTTGGAGGTGAGAGCAGTCCAGAACCACTGGACAACATCTTGATG GCTGCCTTTGAATTCGACATTCACCAAGTGATCAAGGAGTGCAGCATTGCCCTGAGCAACTGGTGGTTCG TAGCTCACCTCACAGACCTTTTGGATCACTGCAGACTCCTCCAGTCACACAATCTCTATTTTGGTTCTAA CATGAGAGAATTCCTCCTTCTGGAATATGCCTCAGGACTGTTTGCTCATCACAGCCTGTGGCAGCTGGGG GTGGATTACTTTGACTACTGCCCGGAGCTGGGCCGAGTTTCCTTGGAGCTGCACATTGAGCGGATTCCTC TCAACACAGAGCAGAAAGCTTTGAAGGTGCTGAGGATTTGTGAGCAGCGGCAGATGACTGAACAAGTTGG AAGCATCTGTAAGATCTTGGCCATGAAGGCTGTTCGTAATAATCGCTTGGGCTCAGCCCTCTCCTGGAGC ATCCGCGCCAAAGATGCTGCCTTCGCCACACTCGTGTCCGACAGATTCCTCCGGGATTACTGTGAAAGAG GCTGCTTTTCTGACTTGGATCTCATTGACAATCTGGGGCCAGCCATGATGCTCAGTGACCGACTGACATT TCTGGGAAAGTACCGGGAGTTCCACAGACTGTACGGGGAGAAGCGCTTTGGTGATGCTGCTTCTCTTCTG CTGTCCCTCATGACCTCTCAGATTGCACCCCGTTCTTTCTGGATGACTCTGCTCACAGATGCCCTTCCTC TTTTGGAACAGAAGCAGGTGATTTTTTCAGCAGAGCAGACCTACGAGCTGATGCGATGCCTGGAAGACTT GGCCTCCAGAAGGCCAGAGTGTGGTGAGCCCGACGCCCAGCGACTGCAGGATGACGACATAGAGACCACC AAGGTGGAGATGCTGAGACTAGCTCTTGCCCGGAATCTTGCTCGGGCAATTATAAGGGAAGGCTCACTGG AGGGTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001025401 |
| Insert Size | 1971 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025401.1, NP_001020572.1 |
| RefSeq Size | 2101 bp |
| RefSeq ORF | 1971 bp |
| Locus ID | 287830 |
| UniProt ID | Q4QQS8 |
| Gene Summary | Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Involved in nephrogenesis.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR208234 | Nup85 (Myc-DDK-tagged ORF) - Rat nucleoporin 85kDa (Nup85), (10 ug) |
CNY 8,460.00 |
|
| RR208234L3 | Lenti ORF clone of Nup85 (Myc-DDK-tagged ORF) - Rat nucleoporin 85kDa (Nup85), (10 ug) |
CNY 10,260.00 |
|
| RR208234L4 | Lenti ORF clone of Nup85 (mGFP-tagged ORF) - Rat nucleoporin 85kDa (Nup85), (10 ug) |
CNY 11,210.00 |
