Arhgef18 (NM_001107115) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207858 representing NM_001107115 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATCAGCCCTGGAGGACCCCACTCCAACCTCACCTGGTTCGAGTTCCTGTCAGAGAGCGAGGATG GTGTGTGCAAGAATGAGAAGACGGACAAGAGCACCAGAGTGAAGCGCAGCCTGAGCTCCCTCCGCAGCAG GGTCACGAGGCAGAAGGAGAAGGGGAAGAACCCAGCACATCTAAAAGATAAGATTCAGGATGTTCCAGGA AAGCGGGAGTGTGTCAACGGGCACCAGCTGGTGCGAGGAACCTTCTCGGGCCACTCCAGCTGTCCTCTGT GTGGCAAACCTTTGCTGAGCTCAGCTTGGTCTCGCACGGCACACCGCTTCCGGCTCCTTGGAGGCCGGGC GGAGGCTGCGAGAGCGACCCGGGAGCCAGCAGGGCGGAGAGCGGCCTGTGAGGGACCCCGCGGAGCCTCC CTGAAGGAGCACCCCCGGACCGCACTTCTGTCTGATGGCAGCAGCCCGGCTCCCTCCCGGAATGTCGGCA TGACCGTCTCTCAGAAAGGGGGTCTCCAGCCAACACCGAGCCCAGCTGGATCTGGGGTGCGACTTGGACC GATCGCTGGAGACATGGATGAAGCTGACTCCGTGTTTTTAAAGCTGAAACAGACGGCTGATGACTCTCTG TCACTCACATCCTCGAATGCTGAGTCTGTTTTCATAGAAGATCCTTACACTGCCTCGCTGAGGTGTGAGA TCGAATCAGATGCCCATGAGTTTGAGGCCGAGTCCTGGAGCCTCTCGGTGGATGTAGCATATGCCAAGAA ACAAAAGAAGGAGGTGGTCAAGAGACAGGACGTCCTGTATGAGCTGATGCAGACAGAGGCGCATCACGTG CGCACGCTGAAGATCATGCTGAAAGTATACTCCAGAGCCCTGCAGGAGGAGCTGCAGTTCAGCGTCCAGG CAGTCAGCCGCCTGTTCCCATGCGCCGACGACTTACTGGACATGCACAGCCACTTCCTCGCCCGCCTTAA GGAGCGCCGGCAGGAGTTCCTAGAGGAGGACAGTGATCGGAATTATGTCATCCAGAAGATCGGAGATGTC CTGGTCCAGCAGTTCTCAGGAGAAACCGGGGAGAGAATGAAGGAGAAATACGGTGTGTTCTGTAGCGGCC ACAAGGATGCGGTCGGGCACTACAAGTTACTGCTGCAGCAGAGCAAGAAGTTTCAAAACTTAATCAAGAA AATTGGCAACTTTTCCATCGTGCGAAGGCTGGGAGTGCAGGAGTGTATCCTTCTGGTCACACAGCGTATA ACCAAGTACCCGGTGCTAGTGGAACGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTACAAGGACC TGAGCCAGGCCCTGAACCTCATCAAAGACATCATCTCACAAGTGGATGCCAAAGTCAGTGAGTGTGAGAA GGACCAGCGCCTCAAGGAGATCGTAGCCAAGATGGACTCGAAGTCATCCGGCAAACTCAAGAACGGGCGA ACCTTCCGAAAGGAGGATATGCTGCAGCGCCAGCTCCACCTGGACAGCACCCTGTGCTGGAAGTCCACGT CGGGGCGCTTGAAAGATGTCCTCGCTGTCCTGTTGACCGATGTGCTTTTGCTGCTGCAAGAAAAGGATCA AAAATACGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCTCTGCAAAAGCTCATTGTGAGAGAG GTAGCCAACGAGGAGAAGGCCATGTTTCTGATCAGCGCCTCCATGCAGGGACCAGAGATGTACGAGATCT ACACCAGTTCCAAAGAGGACAGGAACATCTGGATGGCCCACATCCGACGGGCCGTGGAGAGCTGTCCTGA CGAGGAGGAGGGCATTTTCCTCGAGGCTGAGGAGAGAAGGATGGCTGAAGCCCGCACCATGAAACTCCGG GACTTTCAAGAGCGACTGAGCCTGAAAGACCAGCTGATCGCCCAGAGCCTTCTGGAGAAACAGCAGATCT ACCTGGAGATGGCTCAGCTGAGCGGGCTGGATGAGTCGGCCCAGTGCCGAGGCCTCTTCCGAGGAGGAGG GGACCCCTCTGAGACCCTACGAGGGGAGCAGATTCTCAGATCAGCCATGAGTGAGATTGAGGGCATCCAG AGCCTGATCTGCCGGCACGTGGGCAGCACCAGTAGCCAAGTGGAAGAGGGAGGTGTCTCTGCAGGCCTGC CTCGGAGGGCTGAGACCTTCGGGGGCTATGACAGCGTGGGCAGCCCCAACAAGGGTGGCAGCTTTAAAAG GAAGGTGTACAACAGTGACCCCAGGCCCCAAGACTGGCAAGGGCCTGCTAGCAGCCCGGACTCCATGCCC TGTGACACCACCACCCCCAGTGGCTGCTGCGAGGAATCACCACAGGCTGTGGAGATGCCTAGCGCAGAGA GTAGCCCATGTCTGCCCACTGTCCTGGAATCAGAGCTGGTCCACCGGGTCCAGACCCTGTCGCAGCTGCT TCTTAGTCTCCAGGCAGTCATCGCTCAACAGGATAGCTACGTGGAGATGCAGCGAATGGCCATCCAGGAG CGTGAGAAGCAGTTCCGGCTGCAATCAACGCGTGGGAACCTGCTTCTAGAGCAAGAGCGGCAACGCAACT TTGAGAAGCAGCGTGAGGAGCGCGCGGGTGTGGAGAAGCTGCAAAACCAGCTGCGCCAGGAGCAGCAGCG ATGGGAGCGGGAGCGGGCACGCCAGCAGCAGGAACTGGAGCTGGCAGGCGCGCGACTGCAGGAGAGAGAG GGCGAGGCGCGCCAGCTGCGCCAGCGGCTGGACCAGGAGCGCACAGAGCTGGAGAGACAGCGCCAGGCCT ACCAGCATGATCTGGAGCGTCTGCGCGAGGCCCAGAGAGCAGTGGACCGTGAACGTGAGCGCCTGGAACT ACTGCGCAGGTTCAAGAAACAGAACACTGTACCGGGGGCACTGCCCCCGGAAGTGCTGGCAGAGGCCCAG CCTGCAAGCCACCCTCTCAACTTCAATGGGGATGGGCTGGAGGGCCACTTGGCCCCAGCCAAAGCACCAG GAACACAGGGGAGCATGCTACACGGCACAGGACCTGAGTACGGAGAGCGAGCTGAGGTGGCCCGCTGGGA CAGTGCCCCTGCTGAGAGCCGGCCAGCCAAGAGTGATGTGCCCATCCAGCTGCTCAGTGCCACCAATCAG ATTCAGAGGCAGACAGCCGTGCAGCAGCAGATCCCCACCAAGTTGGCCGCCTCCACCAAGGGGGGCAAGG ATAAGAACAGCAAGAGCAGGGGTTCCCAGCGCTGGGAGAGCTCAGCATCCTTCGACCTGAAGCAGCAGCC ACTCTTCAACAAGCTCATGGGCAAGGACGAGAACGCGTCCCGAAACCGCCGCTCACTGAGCCCCGTCCTA CCTGCAGCCCATGGATCTGCACCTGCCCCAGACCCCTGCTTCCCAGCCCCAAGCCCGGTCCCGGCCGCCA CAGCCCCCGAGGCCTTCAAGTCAGGAGGCACGTCACTGCCACCTGCCTCCCCAGCTCCCTCGCTGCCAAC CACACCGCCCACCATCACAGATGAAGTTGGCAAGGAAGACGTCATCTTCTTCTGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001107115
Insert Size	3555 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107115.1, NP_001100585.1
RefSeq Size	6131 bp
RefSeq ORF	3555 bp
Locus ID	304193

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