Atp12a (NM_133517) Rat Untagged Clone
CAT#: RN207798
Atp12a (untagged ORF) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), (10 ug)
CNY 16,630.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN207798 representing NM_133517
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGGAAAACAGAAATTTATTCTGTAGAGCTCAACGGAACTAAAGACGTCAAGCCAGCAGACCAGA GGGATGACAAAAAGTTCAAAGGAGCGAAGAACAAGGACTTGGAACCCAACAAGAGTCACGAGAAAGAGGA GCTGAAGAAAGAACTTGATCTGGATGATCACAGACTCAGTAATACAGAATTGGAACAGAAATATGGTACC AACATCATCCAGGGTCTCTCCAGCGTCAGAGCTACAGAGCTCCTGGCTCGGGATGGGCCCAACACTCTTA CCCCTCCCAAGCAAACTCCAGAGATTATCAAGTTCCTCAAGCAGATGGTGGGGGGCTTTTCCATCCTCCT GTGGATAGGTGCTGCCTTGTGCTGGATCGCATTTGTGATCCAGTACGTCAACAATTCCGCATCCCTAGAC AACGTCTACTTGGGCGCCATACTTGTCCTGGTTGTTATTTTAACGGGGATTTTTGCTTATTACCAAGAAG CCAAAAGCACCAACATCATGGCCAGCTTTAGTAAGATGATCCCCCAGCAAGCTCTTGTCATCCGAGATGC CGAAAAGAAGGTCATTTCAGCAGAGCAACTGGTGGTGGGGGACGTTGTGGAGATTAAAGGAGGGGACCAG ATCCCTGCAGACATCAGACTGGTATTTTCCCAAGGGTGCAAAGTAGATAACTCCTCTCTCACTGGGGAAT CTGAGCCCCAGGCTCGTTCAACTGAGTTTACCCATGAAAACCCCTTGGAAACCAAGAACATAGGCTTCTA TTCCACAACCTGCCTGGAAGGTACAGCAACTGGCATTGTCATCAACACGGGTGACCGTACCATCATCGGG CGCATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAAACACCCATTGCCATTGAGATCGAGCATTTTG TCCATATTGTGGCAGGAGTGGCTGTGTCCATTGGCATCATTTTCTTCATCACCGCCGTGTGCATGAAGTA CTATGTCCTCGACGCCATCATCTTCCTCATTAGCATCATTGTGGCTAACGTGCCAGAGGGCCTCCTGGCT ACTGTCACTGTTACCCTGTCACTGACGGCAAAACGGATGGCCAAGAAGAACTGCCTGGTAAAGAATCTGG AGGCAGTGGAGACTCTTGGCTCCACCTCCATCATCTGCTCAGACAAGACGGGGACCCTGACCCAGAACAG GATGACTGTGGCTCACTTGTGGTTTGACAATCAGATCTTTGTGGCTGATACTAGTGAAAACCAAACAAAA CAAGCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAAGATAATAACACTGTGTAACCGAGCAG AGTTCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAACTGTGGTTGGAGACGCCTCAGAAACTGC TCTGCTAAAGTTCTCAGAGGTCATTTTGGGTGACGTGATGGGAATTAGGAAAAGAAACCACAAAGTGGCT GAAATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACATGAGACAGAGGACCCTAATGACAAAC GCTTCCTCGTGGTGATGAAAGGAGCCCCTGAGAGGATCTTAGAGAAGTGCAGCACCATCATGATCAATGG CCAGGAGCAGCCACTGGACAAGAGCTCTGCTGATAGCTTCCACACAGCCTACATGGAGCTGGGCGGTCTG GGCGAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGAGCAGTTCCCCCAAAGTTACATCTTTG ATGTCGACTCCGTAAACTTTCCCACTTCCAACTTCTGCTTTGTGGGGCTTCTGTCCATGATCGACCCTCC TCGGTCTACTGTGCCAGACGCAGTCTCCAAATGTCGGAGTGCCGGGATCAAGGTTATCATGGTCACAGGT GATCACCCTATCACCGCCAAAGCTATTGCCAAGAGTGTAGGAATCATATCGGCCAACAACGAGACAGTGG AAGACATCGCGAAACGCCGCAATATCGCTGTGGAGCAGGTCAACAAAAGAGAAGCTAAAGCCGCTGTGGT GACTGGCATGGAGCTGAAGGACATGACCCCAGAACAACTGGATGAGCTTTTGACCAACTACCAAGAAATC GTATTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTGGAGGGCTGTCAGAGGCAGGACGCCATCG TTGCCGTGACGGGTGATGGAGTGAACGACTCTCCAGCACTGAAGAAGGCAGACATCGGGATTGCTATGGG GATAGCAGGTTCTGACGCTGCTAAGAACGCAGCTGACATGGTCTTACTAGACGACAACTTCGCGTCTATA GTCACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAAGAAAACCATTGCGTACACCCTGACAA AGAACATCGCCGAGCTCTGCCCTTTTTTGATCTACATCGTTGCTGGGCTCCCCCTGCCTATTGGAACCAT CACTATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCATTGCCTTGGCCTATGAGAAAGCAGAA AGTGACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAGACTGGTGAACACGCAGCTGGCCATCT ACTCTTACCTTCACATTGGTCTCATGCAAGCTCTGGGAGGTTTCCTCGTCTATTTCACTGTCTATGCACA GCAGGGCTTTTGGCCCACTTCTCTCATCAACCTGAGGGTAGCATGGGAAACAGATGACATAAATGACTTG GAAGACAGCTACGGGCAGGAATGGACAAGGTATCAGAGAAAATACCTGGAATGGACAGGCTCAACGGCTT TCTTTGTTGCCATCATGATCCAACAAATTGCGGATCTGATCATCAGGAAAACCCGGAGGAATTCCATCTT TCAGCAGGGGCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCGCCTCACAGGTCATCGTTGCACTGATC CTCTCCTATGGTCTCGGGAGTGTCCCAGCTCTGAGCTTTACCATGCTCAGGGTTCAGTACTGGTTTGTGG CTGTGCCACATGCCATTTTGATTTGGGTGTATGACGAGATGCGAAAGTTGTTCATCAGGCTCTACCCTGG AAGCTGGTGGGATAAGAACATGTATTATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_133517 |
Insert Size | 3111 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_133517.2, NP_598201.2 |
RefSeq Size | 4019 bp |
RefSeq ORF | 3111 bp |
Locus ID | 171028 |
Gene Summary | may act as a H+,K(+)-ATPase in distal colon; may also play a role in K+ absorption and H+ secretion in the distal nephron [RGD, Feb 2006] Transcript Variant: This variant (1) encodes the longer isoform (a, also known as HKalpha2a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR207798 | Atp12a (Myc-DDK-tagged ORF) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), (10 ug) |
CNY 16,630.00 |
|
RR207798L3 | Lenti ORF clone of Atp12a (Myc-DDK-tagged ORF) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), (10 ug) |
CNY 18,240.00 |
|
RR207798L4 | Lenti ORF clone of Atp12a (mGFP-tagged ORF) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), (10 ug) |
CNY 20,050.00 |