Atp12a (NM_133517) Rat Untagged Clone

CAT#: RN207798

Atp12a (untagged ORF) - Rat ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), (10 ug)



  "NM_133517" in other vectors (3)

CNY 16,630.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN207798 representing NM_133517
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCGGAAAACAGAAATTTATTCTGTAGAGCTCAACGGAACTAAAGACGTCAAGCCAGCAGACCAGA
GGGATGACAAAAAGTTCAAAGGAGCGAAGAACAAGGACTTGGAACCCAACAAGAGTCACGAGAAAGAGGA
GCTGAAGAAAGAACTTGATCTGGATGATCACAGACTCAGTAATACAGAATTGGAACAGAAATATGGTACC
AACATCATCCAGGGTCTCTCCAGCGTCAGAGCTACAGAGCTCCTGGCTCGGGATGGGCCCAACACTCTTA
CCCCTCCCAAGCAAACTCCAGAGATTATCAAGTTCCTCAAGCAGATGGTGGGGGGCTTTTCCATCCTCCT
GTGGATAGGTGCTGCCTTGTGCTGGATCGCATTTGTGATCCAGTACGTCAACAATTCCGCATCCCTAGAC
AACGTCTACTTGGGCGCCATACTTGTCCTGGTTGTTATTTTAACGGGGATTTTTGCTTATTACCAAGAAG
CCAAAAGCACCAACATCATGGCCAGCTTTAGTAAGATGATCCCCCAGCAAGCTCTTGTCATCCGAGATGC
CGAAAAGAAGGTCATTTCAGCAGAGCAACTGGTGGTGGGGGACGTTGTGGAGATTAAAGGAGGGGACCAG
ATCCCTGCAGACATCAGACTGGTATTTTCCCAAGGGTGCAAAGTAGATAACTCCTCTCTCACTGGGGAAT
CTGAGCCCCAGGCTCGTTCAACTGAGTTTACCCATGAAAACCCCTTGGAAACCAAGAACATAGGCTTCTA
TTCCACAACCTGCCTGGAAGGTACAGCAACTGGCATTGTCATCAACACGGGTGACCGTACCATCATCGGG
CGCATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAAACACCCATTGCCATTGAGATCGAGCATTTTG
TCCATATTGTGGCAGGAGTGGCTGTGTCCATTGGCATCATTTTCTTCATCACCGCCGTGTGCATGAAGTA
CTATGTCCTCGACGCCATCATCTTCCTCATTAGCATCATTGTGGCTAACGTGCCAGAGGGCCTCCTGGCT
ACTGTCACTGTTACCCTGTCACTGACGGCAAAACGGATGGCCAAGAAGAACTGCCTGGTAAAGAATCTGG
AGGCAGTGGAGACTCTTGGCTCCACCTCCATCATCTGCTCAGACAAGACGGGGACCCTGACCCAGAACAG
GATGACTGTGGCTCACTTGTGGTTTGACAATCAGATCTTTGTGGCTGATACTAGTGAAAACCAAACAAAA
CAAGCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAAGATAATAACACTGTGTAACCGAGCAG
AGTTCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAACTGTGGTTGGAGACGCCTCAGAAACTGC
TCTGCTAAAGTTCTCAGAGGTCATTTTGGGTGACGTGATGGGAATTAGGAAAAGAAACCACAAAGTGGCT
GAAATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACATGAGACAGAGGACCCTAATGACAAAC
GCTTCCTCGTGGTGATGAAAGGAGCCCCTGAGAGGATCTTAGAGAAGTGCAGCACCATCATGATCAATGG
CCAGGAGCAGCCACTGGACAAGAGCTCTGCTGATAGCTTCCACACAGCCTACATGGAGCTGGGCGGTCTG
GGCGAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGAGCAGTTCCCCCAAAGTTACATCTTTG
ATGTCGACTCCGTAAACTTTCCCACTTCCAACTTCTGCTTTGTGGGGCTTCTGTCCATGATCGACCCTCC
TCGGTCTACTGTGCCAGACGCAGTCTCCAAATGTCGGAGTGCCGGGATCAAGGTTATCATGGTCACAGGT
GATCACCCTATCACCGCCAAAGCTATTGCCAAGAGTGTAGGAATCATATCGGCCAACAACGAGACAGTGG
AAGACATCGCGAAACGCCGCAATATCGCTGTGGAGCAGGTCAACAAAAGAGAAGCTAAAGCCGCTGTGGT
GACTGGCATGGAGCTGAAGGACATGACCCCAGAACAACTGGATGAGCTTTTGACCAACTACCAAGAAATC
GTATTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTGGAGGGCTGTCAGAGGCAGGACGCCATCG
TTGCCGTGACGGGTGATGGAGTGAACGACTCTCCAGCACTGAAGAAGGCAGACATCGGGATTGCTATGGG
GATAGCAGGTTCTGACGCTGCTAAGAACGCAGCTGACATGGTCTTACTAGACGACAACTTCGCGTCTATA
GTCACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAAGAAAACCATTGCGTACACCCTGACAA
AGAACATCGCCGAGCTCTGCCCTTTTTTGATCTACATCGTTGCTGGGCTCCCCCTGCCTATTGGAACCAT
CACTATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCATTGCCTTGGCCTATGAGAAAGCAGAA
AGTGACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAGACTGGTGAACACGCAGCTGGCCATCT
ACTCTTACCTTCACATTGGTCTCATGCAAGCTCTGGGAGGTTTCCTCGTCTATTTCACTGTCTATGCACA
GCAGGGCTTTTGGCCCACTTCTCTCATCAACCTGAGGGTAGCATGGGAAACAGATGACATAAATGACTTG
GAAGACAGCTACGGGCAGGAATGGACAAGGTATCAGAGAAAATACCTGGAATGGACAGGCTCAACGGCTT
TCTTTGTTGCCATCATGATCCAACAAATTGCGGATCTGATCATCAGGAAAACCCGGAGGAATTCCATCTT
TCAGCAGGGGCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCGCCTCACAGGTCATCGTTGCACTGATC
CTCTCCTATGGTCTCGGGAGTGTCCCAGCTCTGAGCTTTACCATGCTCAGGGTTCAGTACTGGTTTGTGG
CTGTGCCACATGCCATTTTGATTTGGGTGTATGACGAGATGCGAAAGTTGTTCATCAGGCTCTACCCTGG
AAGCTGGTGGGATAAGAACATGTATTATTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133517
Insert Size 3111 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133517.2, NP_598201.2
RefSeq Size 4019 bp
RefSeq ORF 3111 bp
Locus ID 171028
Gene Summary may act as a H+,K(+)-ATPase in distal colon; may also play a role in K+ absorption and H+ secretion in the distal nephron [RGD, Feb 2006]
Transcript Variant: This variant (1) encodes the longer isoform (a, also known as HKalpha2a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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