Ppfia2 (NM_001108745) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207506 representing NM_001108745 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCGAAGTGATGCCCACGATTAATGAGGACACCCCAATGAGCCAACGGGGGTCCCAAAGTAGTG GCTCTGACTCGGACTCCCACTTTGAGCAACTGATGGTCAATATGCTAGATGAAAGGGACCGTTTACTGGA CACACTTCGAGAGACTCAGGAAAGCCTCTCGCTTGCCCAGCAAAGACTCCAGGATGTCATCTACGACCGA GATTCACTCCAGAGACAGCTTAATTCAGCCCTGCCTCAGGACATCGAATCTCTAACAGGAGGGCTGACTG GTTCTAAGGGGGCTGATCCACCGGAGTTTGCTGCACTGACTAAAGAGCTGAATGCTTGCCGTGAGCAACT TCTAGAAAAGGAAGAAGAGATTTCAGAGCTAAAAGCTGAAAGAAACAACACAAGACTGTTGCTGGAACAC TTGGAGTGCCTTGTGTCCCGACATGAAAGGTCACTAAGAATGACAGTGGTCAAGCGGCAAGCACAGTCTC CCTCTGGAGTGTCCAGTGAAGTGGAGGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAAGCCTT GGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAGGAACTAGCT GCAGCTAATCAGGAGATTGTTGCCTTGCGTGAGCAAAATGTTCATATTCAAAGAAAAATGGTATCGAGTG AGGGGTCCACGGAGTCAGAACATCTTGACGGGATGGAACCAGGCCAGAAAGTCCATGAAAAGCGTCTATC CAATGGCTCCATAGATTCCACAGATGACACCAGCCAGATCGTCGAGCTGCAAGAATTGCTTGAAAAGCAA AACTATGAAATGGCCCAAATGAAAGACCGCTTAGCAGCTCTTTCTTCCCGAGTGGGAGAAGTGGAACAAG AAGCCGAGACGGCAAGAAAGGACCTCATTAAAACAGAAGAAATGAACACCAAATATCAAAGGGACATCCG AGAGGCCATGGCTCAGAAGGAAGATATGGAAGAAAGAATCACAACTCTGGAGAAGCGTTACCTGAGTGCC CAGAGAGAATCTACCTCCATTCACGACATGAATGATAAACTAGAAAATGAGTTGGCAAACAAGGAAGCCA TCTTGCGGCAGATGGAAGAGAAAAACAGGCAATTGCAAGAGCGTCTTGAGCTGGCTGAGCAAAAGTTACA GCAGACCATGAGGAAAGCTGAAACTTTACCTGAAGTAGAGGCAGAACTTGCTCAGAGAATTGCAGCCCTA ACAAAGGCTGAAGAGAGACATGGAAATATTGAAGAGCGTATGAGACACCTAGAGGGTCAACTTGAAGAGA AGAATCAAGAACTTCAAAGAGCTAGGCAAAGGGAGAAAATGAATGAAGAGCATAACAAGAGATTATCTGA CACCGTGGACAGGCTTCTGACAGAATCCAATGAGCGCCTGCAGCTGCACCTAAAGGAAAGGATGGCTGCC TTGGAAGAGAAGAATGTTTTGATTCAAGAATCAGAAACCTTCAGAAAAAATCTTGAAGAGTCCTTACATG ATAAGGAAAGACTAGCAGAAGAAATAGAGAAGTTGAGGTCTGAACTTGACCAAATGAAAATGAGAACTGG CTCTCTAATTGAACCAACTATATCGAGAACTCATCTAGACACCTCCGCTGAGTTGCGGTATTCAGTTGGG TCCCTCGTAGACAGCCAGTCTGATTACAGAACCACTAAAGTAATACGAAGACCAAGGAGAGGCCGCATGG GTGTGCGAAGAGATGAGCCAAAGGTGAAATCCCTTGGGGATCATGAATGGAACAGGACTCAGCAAATTGG AGTACTAGGCAGTCACCCATTCGAAAGTGACACTGAAATGTCAGATATTGATGATGACGACAGAGAAACG ATTTTCAGCTCAATGGATCTTCTCTCTCCAAGTGGCCATTCTGATGCCCAGACGCTAGCTATGATGCTTC AAGAACAACTGGATGCAATCAACAAAGAAATAAGGCTAATCCAGGAAGAAAAGGAATCTACAGAATTGCG TGCTGAAGAAATCGAGAATAGGGTGGCTAGTGTAAGCTTGGAAGGTCTGAACTTGGCAAGGGTCCATCCT GGTACCTCCATCACTGCTTCTGTTACAGCTTCATCACTGGCCAGTTCATCTCCCCCAAGTGGACACTCAA CGCCAAAGCTTACACCAAGAAGCCCGGCCAGGGAAATGGACCGTATGGGAGTCATGACCCTGCCAAGTGA TCTAAGGAAACATCGGAGAAAGATTGCAGTGGTGGAAGAAGATGGGCGGGAAGATAAAGCAACAATTAAA TGTGAAACTTCTCCTCCCCCAACACCCAGAGCCATCAGAATGACTCACACCCTACCTTCTTCCTACCACA ATGAGGCCCGGAGCAGTTTATCTGCCTCTCTTGAGCCAGATAGACTTGGGCTTGGCAGTGCCAATAGCAG CCAGGACTCTCTTCACAAAGCCCCGAAGAAGAAAGGAATCAAGTCTTCAATTGGGCGTTTGTTTGGTAAA AAAGAGAAGGCTCGACTTGGGCAGCTTCGAGGCTTCATGGAGACAGAAGCTGCAGCACAGGAATCGCTGG GCTTAGGCAAACTTGGCACTCAAGCTGAAAAGGACAGAAGGCTGAAGAAAAAGCATGAACTTCTTGAAGA AGCTCGTAGAAAAGGATTACCCTTTGCCCAGTGGGACGGACCTACTGTGGTTGCGTGGCTGGAGCTCTGG CTGGGAATGCCTGCTTGGTATGTGGCAGCATGCAGAGCCAACGTGAAAAGTGGTGCCATCATGTCGGCTT TATCAGACACTGAAATCCAAAGGGAGATTGGAATCAGCAACCCTCTGCACCGCTTAAAGCTCAGACTAGC CATCCAGGAGATGGTTTCCCTCACAAGCCCTTCAGCTCCTCCGACATCGCGAACTCCTTCAGGCAATGTC TGGGTGACCCATGAAGAAATGGAAAACCTCGCAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCT GGGCCCAGACCCTGGCTTATGGAGATATGAACCATGAGTGGATCGGGAACGAATGGCTTCCCAGCCTCGG ATTACCGCAATACCGAAGTTACTTTATGGAATGCTTGGTTGATGCAAGAATGTTAGATCACCTCACAAAA AAGGATCTTCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTTTACAGTATGGGATTATGT GCTTGAAAAGATTGAATTATGATAGGAAAGAGCTAGAGAGAAGACGAGAAGCAAGTCAACATGAAATAAA AGATGTTTTAGTGTGGAGCAATGATCGAGTTATTCGCTGGATCCAAGCGATTGGACTTCGGGAATATGCC AATAACATTCTTGAGAGTGGAGTGCATGGCTCACTCATAGCCCTGGATGAGAACTTTGACTACAGCAGCC TAGCTCTACTGCTGCAGATTCCCACGCAGAACACCCAGGCAAGGCAGATTCTTGAACGCGAGTATAATAA CCTCTTGGCTCTGGGAACTGAGCGGCGACTGGATGAAAGTGATGACAAGAATTTCAGACGCGGATCAACC TGGCGAAGGCAGTTCCCTCCTCGCGAGGTACACGGAATCAGCATGATGCCTGGATCCTCAGAAACATTAC CAGCTGGATTCAGGTTGACCACAACATCTGGGCAGTCCAGAAAAATGACGACAGATGTTGCTTCATCAAG ACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108745
Insert Size	3756 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108745.1, NP_001102215.1
RefSeq Size	4986 bp
RefSeq ORF	3756 bp
Locus ID	362876

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