Cltc (NM_019299) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207450 representing NM_019299 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGATTCTGCCAATTCGCTTTCAGGAGCATCTCCAGCTCCAGAACCTGGGTATCAACCCAGCAA ACATTGGCTTCAGTACCCTGACTATGGAGTCAGACAAATTCATCTGCATTAGAGAAAAAGTGGGAGAGCA GGCTCAGGTGGTGATCATTGACATGAATGATCCAAGTAATCCGATTCGAAGACCAATCTCAGCAGACAGT GCCATCATGAATCCTGCCAGCAAAGTGATTGCACTGAAAGCTGGAAAAACTCTACAGATATTTAACATTG AAATGAAAAGTAAAATGAAGGCCCACACCATGACCGATGATGTCACCTTCTGGAAATGGATCTCTCTGAA TACGGTTGCTCTTGTTACGGATAATGCAGTTTATCACTGGAGCATGGAGGGGGAGTCTCAGCCAGTGAAG ATGTTTGATCGTCATTCCAGCCTCGCAGGGTGCCAGATCATCAATTACCGTACAGATGCTAAGCAGAAGT GGTTGCTCCTGACTGGCATATCTGCACAGCAAAATCGCGTGGTTGGAGCTATGCAGCTGTATTCTGTAGA CAGGAAAGTCTCTCAGCCCATTGAAGGGCATGCGGCGAGTTTTGCACAATTTAAGATGGAAGGAAACGCA GAAGAATCGACGTTATTTTGTTTTGCTGTGCGGGGTCAGGCAGGGGGGAAGTTACATATCATTGAAGTTG GTACACCACCCACAGGAAACCAACCCTTTCCAAAGAAGGCAGTGGATGTCTTCTTTCCTCCAGAAGCACA AAATGATTTTCCTGTTGCAATGCAGATCAGTGAAAAGCATGACGTGGTGTTCCTGATTACCAAGTATGGT TATATTCACCTCTATGACCTTGAGACTGGCACGTGCATCTACATGAACAGAATCAGTGGAGAAACCATCT TTGTTACTGCTCCTCATGAAGCCACAGCTGGAATAATAGGAGTCAACAGAAAGGGACAAGTCCTATCAGT TTGTGTGGAAGAAGAGAACATAATTCCTTACATCACCAATGTCCTACAAAATCCTGATTTGGCTCTGAGA ATGGCTGTGCGGAACAACCTAGCTGGTGCAGAAGAGCTCTTCGCCCGGAAATTTAATGCTCTCTTCGCCC AGGGAAACTACTCAGAGGCTGCAAAGGTGGCTGCTAATGCACCGAAGGGAATCCTTCGTACTCCAGATAC CATCCGTCGATTCCAGAGTGTCCCAGCTCAGCCAGGTCAAACTTCACCTCTGCTTCAGTATTTTGGAATC CTTTTGGACCAAGGCCAGCTGAACAAATACGAGTCCTTAGAGTTATGTAGACCTGTCCTTCAGCAAGGGC GGAAACAACTTTTGGAGAAATGGCTGAAAGAAGATAAGCTGGAATGTTCTGAAGAACTGGGTGATCTCGT AAAATCTGTGGACCCAACGTTAGCACTGAGTGTATACCTCAGGGCCAATGTCCCTAATAAAGTCATCCAG TGCTTTGCAGAGACAGGTCAAGTCCAGAAGATTGTTTTATATGCTAAAAAAGTTGGCTATACTCCAGACT GGATCTTTCTCCTGAGAAATGTCATGCGGATCAGTCCAGATCAAGGACAGCAGTTTGCCCAAATGTTAGT TCAGGACGAAGAACCTCTTGCTGATATCACACAGATTGTGGATGTCTTTATGGAATACAATCTAATCCAA CAATGTACTGCCTTCTTACTTGATGCTTTGAAGAATAATCGCCCTTCTGAAGGTCCTTTGCAGACGCGAT TGCTTGAGATGAACCTTATGCACGCACCTCAAGTTGCAGATGCCATTCTAGGGAATCAGATGTTCACCCA CTATGACCGGGCTCACATCGCCCAGCTGTGTGAGAAGGCGGGCCTACTGCAGCGTGCATTAGAACATTTC ACCGACTTATATGATATAAAACGAGCAGTCGTTCATACCCATCTTCTCAACCCCGAGTGGTTAGTGAATT ACTTTGGGTCCTTATCAGTTGAAGATTCCCTAGAATGTCTCAGAGCCATGCTGTCTGCTAATATTCGTCA AAATCTACAGATCTGGGTTCAGGTCGCTTCTAAATATCATGAACAGCTGTCAACTCAGTCCCTGATTGAA CTCTTCGAGTCATTCAAGAGTTTTGAAGGCCTCTTTTATTTTCTGGGGTCCATTGTTAACTTCAGTCAAG ACCCAGATGTGCACTTTAAATATATTCAGGCGGCTTGCAAGACTGGACAAATCAAAGAGGTGGAAAGAAT CTGCAGAGAGAGCAACTGCTATGATCCAGAGCGCGTCAAGAATTTCCTCAAGGAGGCGAAACTCACAGAT CAGTTGCCACTCATCATCGTCTGTGACCGCTTTGACTTCGTCCATGACCTGGTGCTGTATTTATACAGAA ACAGTCTTCAGAAGTACATAGAGATATATGTCCAGAAAGTAAATCCAAGTCGACTTCCTGTGGTTATTGG AGGATTGCTGGATGTTGACTGCTCTGAAGATGTCATTAAAAATTTGATTCTTGTTGTGAGAGGTCAATTC TCTACTGATGAGCTTGTTGCTGAAGTTGAGAAAAGAAACAGATTGAAGCTGCTTCTTCCTTGGCTTGAGG CTAGAATTCATGAGGGCTGTGAGGAGCCTGCTACACACAATGCGTTAGCCAAAATATACATCGACAGCAA TAACAACCCAGAGAGATTCCTTCGTGAAAACCCCTACTATGACAGTCGTGTCGTGGGAAAGTACTGTGAG AAGAGAGACCCACATCTGGCCTGTGTTGCTTATGAACGTGGCCAGTGTGATCTGGAACTAATCAACGTTT GCAATGAGAATTCCCTCTTCAAAAGTCTTTCTCGCTACCTGGTCCGTCGAAAGGATCCAGAGTTGTGGGG TAGTGTGCTACTGGAAAGCAATCCATACAGAAGACCATTAATTGACCAGGTCGTACAGACAGCCTTGTCT GAAACTCAGGACCCAGAAGAAGTGTCAGTAACTGTCAAGGCTTTTATGACAGCAGATCTTCCTAATGAAC TCATTGAGCTGCTGGAGAAAATTGTCCTTGATAACTCTGTATTCAGTGAACACAGGAATCTGCAAAACCT CCTCATCCTCACTGCAATTAAGGCTGACCGAACCCGAGTTATGGAGTATATTAACCGCCTGGACAATTAT GATGCGCCAGATATTGCCAACATTGCCATCAGCAATGAGCTCTTCGAAGAAGCATTTGCTATTTTCCGGA AGTTTGATGTCAATACTTCAGCAGTGCAGGTGTTAATTGAACATATTGGAAACCTGGATCGTGCATATGA GTTTGCTGAACGCTGCAATGAGCCTGCTGTTTGGAGTCAACTCGCAAAAGCTCAGTTGCAGAAAGGGATG GTGAAGGAAGCCATTGATTCTTACATCAAAGCAGATGATCCTTCGTCTTACATGGAAGTTGTTCAGGCCG CCAATACTAGTGGAAACTGGGAAGAATTGGTGAAGTATTTGCAGATGGCACGCAAGAAGGCCCGAGAGTC CTACGTGGAGACAGAATTGATATTTGCACTGGCTAAAACAAACCGTCTTGCAGAATTAGAAGAGTTCATC AATGGGCCAAATAATGCTCATATTCAGCAAGTTGGTGACCGTTGTTATGATGAGAAAATGTATGATGCTG CTAAGTTGTTGTACAATAATGTTTCTAACTTTGGACGTTTGGCGTCTACCCTAGTTCACCTTGGCGAGTA TCAGGCAGCAGTTGATGGTGCTAGAAAAGCTAATAGTACTCGAACATGGAAAGAGGTCTGCTTTGCCTGT GTAGATGGGAAAGAGTTTCGCCTTGCTCAGATGTGTGGACTCCATATTGTTGTGCATGCAGATGAATTAG AGGAGCTTATCAACTACTATCAGGATCGTGGATATTTTGAAGAACTCATCACCATGTTGGAGGCAGCCTT GGGACTAGAGCGAGCTCACATGGGAATGTTCACAGAGCTAGCAATTTTGTATTCTAAATTCAAGCCACAG AAAATGAGGGAACACCTAGAGTTGTTTTGGTCAAGAGTGAATATTCCTAAGGTGCTGAGAGCCGCAGAGC AAGCCCATCTTTGGGCAGAGCTGGTGTTTCTGTATGACAAGTATGAAGAGTATGATAATGCCATCATCAC TATGATGAATCACCCCACCGACGCATGGAAGGAAGGGCAGTTCAAAGACATCATCACCAAGGTGGCTAAT GTGGAACTGTACTACAAAGCAATCCAGTTCTACTTAGAATTCAAGCCCTTGTTGTTAAATGACTTGCTCA TGGTGCTGTCTCCACGGTTGGCTCATACTCGTGCAGTCAATTATTTCAGCAAGGTTAAACAGCTACCACT GGTGAAACCATATTTACGCTCAGTTCAGAACCACAACAACAAGTCCGTGAACGAATCCCTGAACAACCTC TTTATCACAGAGGAGGATTACCAGGCTCTGCGAACATCAATAGATGCTTATGACAACTTTGACAACATCT CACTTGCTCAACGTTTGGAAAAACATGAACTCATTGAATTCAGAAGAATTGCTGCTTATCTCTTCAAAGG AAACAATCGCTGGAAACAGAGTGTGGAGCTGTGCAAGAAAGACAGCCTTTACAAGGATGCAATGCAGTAT GCCTCTGAATCGAAAGACACAGAATTGGCTGAAGAACTCCTGCAGTGGTTTCTGCAGGAAGAGAAGAGAG AATGCTTTGGAGCTTGTCTCTTCACCTGCTACGATCTTTTAAGGCCAGATGTTGTCCTGGAAACTGCATG GAGACATAATATCATGGATTTTGCCATGCCCTATTTCATCCAGGTCATGAAGGAGTATTTGACCAAGGTG GATAAATTAGATGCTTCGGAGTCACTGAGAAAAGAAGAAGAACAAGCTACAGAGACACAGCCCATTGTTT ATGGTCAGCCCCAGCTGATGCTAACAGCCGGACCCAGTGTGGCAGTCCCTCCCCAGGCACCTTTTGGTTA TGGTTATACTGCACCACCATATGGGCAGCCACAGCCTGGCTTTGGGTACAGCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019299
Insert Size	5028 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019299.1, NP_062172.1
RefSeq Size	6071 bp
RefSeq ORF	5028 bp
Locus ID	54241
UniProt ID	P11442
Gene Summary	heavy chain component of clathrin triskelions in coated vesicles [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.