Actr2 (NM_001009268) Rat Untagged Clone

CAT#: RN207334

Actr2 (untagged ORF) - Rat ARP2 actin-related protein 2 homolog (yeast) (Actr2), (10 ug)



  "NM_001009268" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC95085
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN207334 representing NM_001009268
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACTGGGTTTGTGAAGTGTGGATATGCAG
GCTCTAACTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATTATCAGATCAACCACCAAAGT
GGGAAACATTGAAATCAAGGATCTCATGGTTGGCGATGAGGCAAGTGAGCTGCGCTCCATGTTGGAGGTG
AACTACCCGATGGAGAACGGCATCGTGCGCAACTGGGACGACATGAAGCACCTGTGGGACTACACATTCG
GGCCAGAGAAGCTCAATATAGACACCAGGAGCTGCAAGATCTTACTTACAGAACCCCCAATGAATCCAAC
CAAGAACAGAGAGAAGATTGTCGAGGTAATGTTTGAAACTTACCAGTTTTCTGGTGTGTATGTAGCCATC
CAAGCAGTTCTGACTTTGTATGCTCAAGGTTTACTGACTGGTGTGGTAGTGGACTCTGGAGATGGTGTCA
CTCACATTTGCCCAGTATATGAAGGCTTTTCCCTCCCTCACCTTACAAGGAGGCTGGATATTGCTGGGAG
GGATATTACCAGGTATCTTATCAAGCTGCTGCTGTTGCGAGGATATGCCTTCAACCATTCTGCTGACTTT
GAGACAGTTCGCATGATTAAAGAAAAACTTTGTTATGTGGGTTACAATATTGAGCAAGAGCAGAAGCTGG
CCTTAGAGACCACAGTGCTAGTTGAGTCATACACTCTTCCAGATGGACGTATTATTAAGGTTGGAGGAGA
AAGATTTGAAGCACCAGAAGCTTTATTTCAGCCTCATTTGATCAATGTTGAGGGGGTTGGTGTTGCTGAA
TTGCTTTTTAACACAATCCAGGCAGCCGACATTGATACCAGATCTGAATTTTATAAGCACATTGTGCTTT
CTGGAGGTTCTACCATGTATCCTGGCCTGCCATCGAGGTTGGAACGAGAGCTTAAACAGCTTTACCTAGA
ACGAGTTCTGAAAGGAGATGTGGAGAAACTTTCGAAATTTAAGATCCGCATTGAAGACCCGCCTCGCAGG
AAGCACATGGTGTTCTTGGGTGGCGCAGTCCTAGCAGACATCATGAAAGACAAAGACAACTTCTGGATGA
CCAGACAAGAGTACCAAGAAAAGGGTGTCCGTGTGCTGGAGAAACTCGGTGTGACTGTTCGATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001009268
Insert Size 1185 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001009268.1, NP_001009268.1
RefSeq Size 1326 bp
RefSeq ORF 1185 bp
Locus ID 289820
UniProt ID Q5M7U6
Gene Summary ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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