Skiv2l (NM_213559) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207109 representing NM_213559 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGACCGAGCGACTCGTGCTGCCTCCCCCAGACCCCCTGAACCTGCCCCTTCGAGCTCTGGAAG TGGGGTGCACTGGCCGTTGGGAGCTGCTAAACGTGCCAGGGCCTCCAGAGAGTACTCTTCCCCATGGCCT CCCTCCCTGTGCCCCAGATCTGTGCCAGGAAGCAGAGCAGTTGTTTCTGTCGTCTCCGGCCTGGCTTCCT CTACACGGTGTGGAGCACTCAGCTCGAAAATGGCAGAGGAAGACAGATCCCTGGTCGCTTCTGGCTGCTC TGGGGACTCCAGTCCCATCCGACCTTCAGGCCCAGAGACACCCAACCACAGGCCATATACTGGGCTATAA GGAGGTCCTGCTGGAGAACACGAACCTGTCGGCCACCACCTCCTTGTCCCTTCGCCGGCCTCCAGGGCCA GCCTCCCAGTCGCTATGGGGCAATCCGACACAGTACCCTTTCTGGCCGGGTGGGATGGACGAGCCCAGCA TAACTGATCTGAGTACTCGGGAGGAAGCCGAGGAGGAGATAGACTTTGAGAAAGATCTTCTCACCGTTCC ACCCGGCTTCAAGAAAGGGGTGGATTTTGCCCCCAAAGCTCCTGTGCCAGGTTTGCTCAGCCTCAGCCGT CTGCTGGAGCCTCTGGATCTGAGTGGAGGTGATGAGGGTGAGGGAGAGGCAGCAGGAGGTCCTAGAGGGG ATGCGGCCTCAGCCTCTCCCTCCAGCACTCCCCTGATCCGGGCAAGCAGCTTGGAGGACCTAGTGTTGAA GGAAGCTTCCACAGTCGTATCCACCCTGGAGCCCCTCAAACCTCCACCTCAGGAACAGTGGGCTGTGCCT GTGGACGTCACCTCCCCCGTGGGTGATTTTTACCGTCTCATCCCTCAGCCGGCCTTCCAGTGGGCGTTCG AGCCAGATGTGTTTCAGAAGCAGGCCATCCTGCACTTGGAGCAGCACGACTCTGTCTTTGTTGCAGCTCA CACATCCGCTGGGAAGACGGTGGTGGCCGAGTATGCCATTGCCCTGGCCCAGAAACACATGACCCGTACC ATCTACACTTCTCCTATCAAAGCCCTGAGCAACCAGAAGTTTCGGGACTTCCGAAACACATTCGGGGATG TGGGACTGCTCACGGGGGATGTGCAGCTGCACCCAGAGGCCTCCTGCCTCATCATGACCACGGAGATCCT TCGCTCCATGCTGTACAGTGGCTCAGACGTCATCCGAGACCTGGAGTGGGTCATTTTTGATGAAGTCCAC TATATCAATGATGCTGAGCGTGGGGTCGTGTGGGAGGAGGTGCTCATTATGCTCCCTGAGCACGTCTCCA TCATCCTGCTGAGTGCCACCGTCCCCAACGCCCTGGAGTTTGCTGACTGGATCGGGAGGCTGAAGCGGCG GCAGATCTATGTGATCAGCACGGTGGCCCGTCCCGTCCCCTTGGAGCACTATCTCTTCACAGGAAACAGC CCCAAGACACAGGGCGAACTCTTCCTTCTGCTGGACTCCCGAGGGGCCTTCCACACCAAGGGGTACTACG CTGCCGTGGAGGCCAAGAAAGAGAGGATGAGCAAACACGCCCAGACCTTCGGGGCCAAGCAGCCCACACA CCAGGGTGGGCCTGCGCAGGACCGTGGGGTGTACCTGGCCTTGCTGGCTTCCCTCCGGACCCGGGCTCAG TTGCCTGTGGTAGTGTTCACCTTCTCCCGAGGCCGCTGTGATGAACAGGCTTCAGGTCTCACCTCCCTAG ACCTCACAACAAGCTCAGAGAAGAGTGAGATCCACCTCTTCCTGCAGCGCTGTCTTGCCCGGCTCCGAGG CTCTGACCGACAGCTCCCCCAGGTCCTGCACATGTCGGAGCTCCTCCGCCGAGGCCTGGGTGTGCACCAC AGCGGCATCCTGCCCATCCTTAAGGAGATTGTGGAGATGCTTTTCAGCCGGGGCCTAGTCAAGGTCCTGT TTGCCACCGAGACTTTTGCCATGGGGGTAAACATGCCAGCCAGGACAGTGGTGTTTGACTCCATGCGCAA ACATGATGGCTCCACCTTCCGGGACCTGCTGCCTGGAGAGTACGTGCAGATGGCAGGCCGGGCGGGCCGG AGGGGCCTGGACCCCACGGGCACCGTCATCCTTCTCTGTAAGGGCCGAGTGCCTGAGATGGCAGATCTAC ACCGCATGATGATGGGGAAACCATCACAGCTTCAGTCCCAGTTCCGCCTCACATATACCATGATCCTCAA CCTGCTGCGGGTGGACGCCCTCAGAGTGGAAGACATGATGAAGAGGAGCTTCTCAGAGTTCCCCTCCCGC AAGGACAGCAAGGCCCATGAGCAGGCTCTGGCTGACCTCACCAAGAGGCTGGGGGCTTTGGAGGAGCCTG ATGTGACTGGTCAGTTGGCTGACCTGCCTGAGTATTACAGCTGGGCGGAGGAGCTGACAGAGACCCGGAA CATGATCCAGCGACGCATCATGGAGTCTGTGAACGGGCTGAAATCTCTGTCAGTGGGAAGGGTGGTGGTT GTGAAGAATGAAGAGCATCACAATGCGCTGGGTGTGATCCTGCAGGTCTCCTCAAACTCCACTAGCCGAG TATTTACAACCTTGGTCTTGTGTGATAAACCTGCTGTGTCTGAGAACCCAAGGGACAAGGGGCCAGCCAC TCCAGATGTGCCCCACCCAGATGACCTTGTAGGCTTCAAGTTGTTCCTGCCTGAAGGGCCCTGCGAGCAC ACTGTGGCCAAGCTCCAGCCAGGGGATGTGGCTGCCATCTCTACCAAGGTGCTCCGGGTGAATGGGGAGA AGATTTCTGAGGACTTCAGCAAGAGGCAGCAACCGAAATTCAGGAAGGACCCTCCCCTCGCGGCCGTGAC CACTGCTGTTCAGGAGCTGCTGCGTCTGGCCCAGGCCTATCCAGCAGGACCCCCAACCCTCGACCCTATC AACGACCTGCAACTGAAGGATGTGGCAGTGGTAGAAGGTGGGCTCCGGGCCCGGAAGCTGGAGGAGCTGA TCCGAGGCGCTCAGTGTGTACACAGCCCCCGGTTTCCTGCCCAGTATGTGAAGCTACAGGAGCGAATGCA GATTCAGAAGGAAATGGAGAGGCTGCGCTTCCTCCTGTCTGACCAGTCGCTGTTGCTGCTCCCCGAGTAC CACCAGCGTGTAGAGGTGCTTCGGACCCTGGGCTATGTGGATGAGGCTGGCACAGTGAAGCTGGCGGGCC GGGTGGCCTGTGCCATGAGCAGCCATGAGCTGCTCCTCACTGAACTCATGTTTGACAATGCACTGAGTGC TCTGCGACCAGAGGAAATAGCAGCTCTGCTCTCTGGTCTGGTGTGCCAGAGCCCCGGAGACCCTGGGGAT CAGCTGCCAAGCACCCTCAAACAGGGGGTAGAACGCGTCAAAGCTGTGGCCAAGCGGATTGGTGAGGTTC AGGTGGCCTGCGGCCTGAACCAGACGGTGGAGGAATTTGTGGGGGAGCTGAATTTTGGGCTGGTGGAAGT TGTTTATGAGTGGGCTCGGGGTATGCCCTTCTCCGAGTTGGCAGGGCTCTCGGGGACCCCCGAAGGCTTG GTTGTCCGCTGTATCCAGCGCCTGGCTGAGATGTGCCGCTCACTTCGAGGGGCGGCCCGTTTGGTAGGAG AGCCTGTGTTAGGGGCCAAGATGGAGACGGCAGCCACCTTGCTCCGGAGGGACATCGTCTTCGCAGCCAG CCTCTATACCCAGTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_213559
Insert Size	3726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_213559.2, NP_998724.1
RefSeq Size	3912 bp
RefSeq ORF	3726 bp
Locus ID	294260
Gene Summary	putative RNA helicase; member of a family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD); rat mouse and human homologs are all located within the MHC class III region [RGD, Feb 2006]

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