Ubn2 (NM_001134553) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1310722
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207089 representing NM_001134553 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCGCGCAGAGTCGCGTTCATTAGCCTGTCACCGGTGAGGCGGCGCGAGGCCGACTTCGCGG GCGCAGAGCGCGAGCCCCCCCGACTGGAGCCGCAGCCGTACCGCGAGCCGGCCCGGGCGGAGCCGGCCCC CCGCGCTGACGCTCAGCCCCCGGCGCGCGACAAGCCGCTCCCCCAGCGCGAGGTCAGCCGCGCCGAGCCG CCCATGGCGCTGCAGCGCGAGCCCCCGCGGCCCGAGCCGCCGCCGCCGCCGCTGCCCCTGCAGACTCCCC CTCCGCGGGAGTCGGCTTCCCGGGCGGAGCCGCCCCCGCGACCGCCGAAGGAGACTGTTCGCCTGGAGCT GGTGCTCAAGGACCCCACGGACGAAAGCTGCGTGGAGTTCAGCTACCCGGAGCTGCTGCTGTGCGGAGAA CAACGGAAGAAACTTGTTCATACAGAAGATCCATTTACTGATGAGCATAAGGAGAGACAAGAAGTGGAAA TGTTGGCTAAGAAGTTTGAAATGAAATACGGTGGGAAAGCCCGTAAACACCGGAAGGATCGGCTGCAGGA TTTAATTGATATAGGCTTTGGCTACGATGAGACAGACCCGTTTATTGATAACTCAGAGGCTTATGACGAA TTAGTCCCTGCTTCTCTAACAACAAAATATGGAGGCTTCTATATCAACACTGGCACTCTGCAGTTTCGCC AAGCTTCAGATACTGAAGAAGATGATTTTACAGATAACCAGAAGCACAAGCCACCCAAGGTTCCCAAAAT AAAAGATGATGATATTGAGGCAAAGAAGCGGAAGCGGAAAGAGGAAGGGGAAAAAGAAAAGAAGCCAAGG AAAAAAGTACCCAAACAACTGGGAGTTGTGGCTCTCAATTCACACAAGTCTGAAAAAAAGAAAAAACGAT ATAAAGATTCCCTTTCTTTGGCTGCCATGATACGAAAATTCCAAAAAGAGAAGGATGCCTTGAAGAAGGA GTCTACCCCTAAAGTCCCAGTGATCCCATCAACTTCCTCTCTGCCTAAGCCCCCTTGTGCTGCCACAACC CTGGGGGATGATATCCCGGACTTAAGTCTGAACAGTGCTGATCCTGACCTCCCTATCTTTGTTAGCACAA ATGAGCATGAACTATTTCAGGAAGCTGAAAATGCCCTAGAGATGCTGGATGATTTTGACTTTGACAGATT ACTGGATGCTACTTCTGATGGAAGTCCCCTCTCTGAGTCAGGGGGAGAAAATGGAAACACCACCCAGCCC ACCTTCGCCTCTCAGGTTGTGCCCAAGGTGGTTCCTACACTTCCAGATGGTCTACCTGTGCTTCTGGAGA AACGCATTGAGGACCTTCGTGTAGCTGCCAAACTTTTTGATGAAGAAGGAAGGAAAAAATTCTTTACACA AGATATGAATAATATTCTTCTAGACATCGAGTTACAGCTACAAGAACTAGGTCCTGTCATCCGTAGTGGT GTCTACTCCCATCTTGAAGCATTTGTGCCATGCAATAAGGAAACACTGGTAAAACGTCTAAAGAAGTTAC ATCTTAACGTCCAGGATGATCGTTTAAGAGAACCTCTGCAGAAACTGAAGCTGGCTGTTAGCAATGTCAT GCCTGAACAGCTGTTTAAATACCAGGAGGACTGCCAGGCTCGCAGTCAAGCCAAATGTGCCAAGTTGCAA GCAGATGAAGAACGAGAAAAAAATGGATCCGATGATGATGATGATGAGAAACCAGGGAAGCGAGTCATAG GCCCACGAAAGAAGTTCCACTGGGATGACACCATCAGAACTTTGTTATGTAACCTTGTTGAAATCAAATT GGGATGCTATGAACTAGAGCCAAATAAAAGCCAGTCTGCTGAGGATTATCTTAAATCCTTCATGGAGACA GAAGTGAAGCCACTGTGGCCTAAGGGCTGGATGCAGGCAAGAATGCTTTTTAAGGAAAGCCGGAGTGTAC ATAATCATCTTACTTCTGCTCCGGCAAAGAAAAAGGTGATTCCTGCATCAAAGCCCAAAGTCAAGGAGGT GATGGTAAAGACCCTTCCTGTTCGTTCTTTCCCTACTATGCTGAAGGAATGTAGCCCCAAAAAAGACCCC AAAGCGCCTGCATCCGTGGTGGCTTCAGGTGGTTGTCCTTGCACGAGTTCCAGCACATCGATCGTTGCCT CAGCCAGCTCTAGCTCTACACCAGCCCAAGAAACCATCTGCCTGGATGACTCCCTAGATGAAGACCTTTC TCTCCCCTCAGCCTCTCTGGATCTTGTATCTGAAGCTTTAGCTGTCATCAACAATGGGAACAAGGGCCCC TCAGTCAGTTCAAGGCTAAATGTGCCAACCACAAAACCTCGTCCAGGCCTGAGAGAAGAAAAGCTAGCAA GTATCATGAGTAAGTTACCACTGGCTACTCCCAAAAAACTAGATTCTCCTCAGACTGCTCACTCATCAAG TCTCATTGCTGGTCACACGGGGCCAGTACCAAAGAAACCCCAGGACTTAGCTCACACTGGTATTTCTTCA GGCCTTATTGCTGGTTCTTCAATTCAGAACCCTAAAGTTTCCTTAGAACCTTTGCCAGCCAGGCTGCTCC AGCAAGGACTGCAAAGGTCAAGCCAGATACATGCTTCCTCTTCACAGACTCATGTCTCCTCCTCCCAAGC CCAAGCTGCTGCCTCCTCTCATGCTCTGGGAACATCAGAGGCTCAAGATGCTTCTTCGTTAACACAAGTA ACAAAGGTGCACCAGCACTCAGCTGTCCAGCAGAACTACGTGTCTCCTTTACAAGCTACCATTAGTAAAT CACAGACCAACCCAGTGGTGAAATTAAGTAATAACCCCCAACTTTCCTGTTCGTCCCAGTTACTCAAGAC TTCAGAGAAGCCACTGATGTATCGCCTCCCTTTGTCTACCCCATCACCTGGAAATGGTTCTCAGGGGTCT CACCCCCTGGTTTCTAGGACAGCACCGAGTACCACTACCTCCAGTAACTATTTAGCCAAGGCTATGGTGT CACAAATCTCCACGCAGGGTTTCAAATCTCCCTTCTCAATGGCCGCATCTCCCAAACTTGCCGCATCTCC CAAACCTGCCACATCTCCTAAACCTTTGACCTCACCTAAGCCTTCTGTTTCACCCAAGCCCTCTCTATCA GCTAAGCCTTCAGTATCTACTAAACTGATTTCTAAATCCAACCCAACTCCCAAGCCTGCTGTATGCCCGA GTTCTTCTAGTCCAAACACACTAGTAGCCCAGAGTAGCCACTCCACAAGTAACAACCCTGCCCATAAACA GCCCAGTGGAATGAACATCAGCAGACAGTCTCCCACTCTAAATTTGTTGCCCTCAAATCGCACTTCTGGC CTTCCGACTACAAAAACTCTTCAGGCCCCTTCTAAACTAACAAACTCATCGTCCGCTGGAACTGTTGGGA AGAACAGCTTGAGTGGAATCCCAGTGAATGTACCTGCCAGCAGAGGTAGCAACCTTAACTCAAGTGGAGC TAATAGGACTAGTCTATCTGGGGGAACAGGAAGTGGAACACAGGGTGCTACTAAACCGTTGTCTACTCCA CATAGACCAACCTCTGCCTCAGGGTCTTCAGTGGTAACAGCCAGTGTGCAGTCTACAGCAGGAGCATCAT TATTGGCTAATGCCTCACCTCTGACTCTCATGACATCACCTTTGTCTGTAACAAATCAAACTGTGACTCC CTTTGGGATGCTGGGTGGCCTTGTTCCAGTGACCATGCCCTTCCAGTTTCCCTTGGAGCTTCTTGGCTTT GGAACGGACACAGCTGGAGTGACAGCCACCTCGGGATCTACCTCAGCGGCTCTCCATCATGGCCTAACTC AGAATTTACTAAAGAGTTTACAGCCAGGAACTCAGCACGCAGCAACCCTTCCCCACTCACCTCTGCCTAC ACACTTACAGCAAGCATTTAATGATGGAGGCCAAAGTAAAGGGGACACTAAATTACCACGGAAACCTCAG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134553
Insert Size	3993 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134553.1, NP_001128025.1
RefSeq Size	5101 bp
RefSeq ORF	3993 bp
Locus ID	312248
UniProt ID	D4A666

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