Srd5a3 (NM_001013990) Rat Untagged Clone

CAT#: RN206628

Srd5a3 (untagged ORF) - Rat steroid 5 alpha-reductase 3 (Srd5a3), (10 ug)



  "NM_001013990" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1308828; S5AR 3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206628 representing NM_001013990
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGTTGGGCGGGGGCCGAGCTCTCGGTCCTGAACCCGCTGCGTGCGCTGTGGCTGTTGCTGGCCG
CCGCCTTCCTGCTCGCACTGCTGCTGCAGCTGGCGCCCGCCAGGCTGCTACCGAGCTGCGCGCTCTTCCA
GGACCTCATCCGCTACGGGAAGACCAAGCAGTCCGGCTCGCGGCGCCCCGCCGTCTGCAGGGCCTTCGAC
GTCCCCAAGAGGTACTTTTCTCACTTCTACGTCGTCTCAGTGTTATGGAATGGCTCCCTGCTCTGGTTCC
TGTCTCAGTCTCTGTTCCTGGGAGCGCCGTTTCCAAGCTGGCTTTGGGCTTTGCTCAGAACTCTTGGGGT
CACGCAGTTCCAAGCCCTGGGGATGGAGTCCAAGGCTTCTCGGATACAAGCAGGCGAGCTGGCTCTGTCT
ACCTTCTTAGTGTTGGTGTTCCTCTGGGTCCATAGTCTTCGGAGACTCTTCGAGTGCTTCTACGTCAGCG
TCTTCTCTAACACGGCCATTCACGTCGTGCAGTACTGTTTCGGGCTGGTCTACTATGTCCTTGTTGGCCT
GACCGTACTGAGCCAAGTGCCCATGAATGACAAGAACGTGTACGCTCTGGGGAAGAATCTACTGCTACAA
GCTCGGTGGTTCCACATCTTGGGAATGATGATGTTCTTCTGGTCCTCTGCCCATCAGTATAAGTGCCACG
TCATTCTCAGCAATCTCAGGAGAAATAAGAAAGGTGTGGTCATCCACTGCCAGCACAGAATCCCCTTTGG
AGACTGGTTCGAGCTTTATGGTGAAGAGCGCAGCCCAGGTGACAGGTTCCCTTCCTCGAGACGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001013990
Insert Size 837 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001013990.1, NP_001014012.1
RefSeq Size 1370 bp
RefSeq ORF 837 bp
Locus ID 305291
UniProt ID Q5RJM1
Gene Summary Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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