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Srd5a3 (NM_001013990) Rat Tagged ORF Clone
CAT＃: RR206628
- TrueORF®
Srd5a3 (Myc-DDK-tagged ORF) - Rat steroid 5 alpha-reductase 3 (Srd5a3), (10 ug)
"NM_001013990" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1308828; S5AR 3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206628 representing NM_001013990
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGTTGGGCGGGGGCCGAGCTCTCGGTCCTGAACCCGCTGCGTGCGCTGTGGCTGTTGCTGGCCG CCGCCTTCCTGCTCGCACTGCTGCTGCAGCTGGCGCCCGCCAGGCTGCTACCGAGCTGCGCGCTCTTCCA GGACCTCATCCGCTACGGGAAGACCAAGCAGTCCGGCTCGCGGCGCCCCGCCGTCTGCAGGGCCTTCGAC GTCCCCAAGAGGTACTTTTCTCACTTCTACGTCGTCTCAGTGTTATGGAATGGCTCCCTGCTCTGGTTCC TGTCTCAGTCTCTGTTCCTGGGAGCGCCGTTTCCAAGCTGGCTTTGGGCTTTGCTCAGAACTCTTGGGGT CACGCAGTTCCAAGCCCTGGGGATGGAGTCCAAGGCTTCTCGGATACAAGCAGGCGAGCTGGCTCTGTCT ACCTTCTTAGTGTTGGTGTTCCTCTGGGTCCATAGTCTTCGGAGACTCTTCGAGTGCTTCTACGTCAGCG TCTTCTCTAACACGGCCATTCACGTCGTGCAGTACTGTTTCGGGCTGGTCTACTATGTCCTTGTTGGCCT GACCGTACTGAGCCAAGTGCCCATGAATGACAAGAACGTGTACGCTCTGGGGAAGAATCTACTGCTACAA GCTCGGTGGTTCCACATCTTGGGAATGATGATGTTCTTCTGGTCCTCTGCCCATCAGTATAAGTGCCACG TCATTCTCAGCAATCTCAGGAGAAATAAGAAAGGTGTGGTCATCCACTGCCAGCACAGAATCCCCTTTGG AGACTGGTTCGAGCTTTATGGTGAAGAGCGCAGCCCAGGTGACAGGTTCCCTTCCTCGAGACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206628 representing NM_001013990
Red=Cloning site Green=Tags(s) MAGWAGAELSVLNPLRALWLLLAAAFLLALLLQLAPARLLPSCALFQDLIRYGKTKQSGSRRPAVCRAFD VPKRYFSHFYVVSVLWNGSLLWFLSQSLFLGAPFPSWLWALLRTLGVTQFQALGMESKASRIQAGELALS TFLVLVFLWVHSLRRLFECFYVSVFSNTAIHVVQYCFGLVYYVLVGLTVLSQVPMNDKNVYALGKNLLLQ ARWFHILGMMMFFWSSAHQYKCHVILSNLRRNKKGVVIHCQHRIPFGDWFELYGEERSPGDRFPSSRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013990 |
| ORF Size | 834 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013990.1, NP_001014012.1 |
| RefSeq Size | 1370 bp |
| RefSeq ORF | 837 bp |
| Locus ID | 305291 |
| UniProt ID | Q5RJM1 |
| MW | 31.8 kDa |
| Gene Summary | Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206628 | Srd5a3 (untagged ORF) - Rat steroid 5 alpha-reductase 3 (Srd5a3), (10 ug) |
CNY 3,990.00 |
|
| RR206628L3 | Lenti ORF clone of Srd5a3 (Myc-DDK-tagged ORF) - Rat steroid 5 alpha-reductase 3 (Srd5a3), (10 ug) |
CNY 6,080.00 |
|
| RR206628L4 | Lenti ORF clone of Srd5a3 (mGFP-tagged ORF) - Rat steroid 5 alpha-reductase 3 (Srd5a3), (10 ug) |
CNY 6,650.00 |
