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Pde5a (NM_133584) Rat Untagged Clone
CAT＃: RN206532
Pde5a (untagged ORF) - Rat phosphodiesterase 5A, cGMP-specific (Pde5a), (10 ug)
CNY 10,640.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | PDE5A2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN206532 representing NM_133584
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCCCTTTGGAGACAAAACGAGAGACATGGTCAATGCATGGTTTTCAGAGAGAGTTCACAACATTC CTGTGTGCAAGGAAGGCATCCGGGCCCACACCGAGTCCTGCTCTTGCTCTCTGCCTCAAAGTCCTCATGC AGATAATACCACCCCTGGAGCACCAGCCAGAAAAATCTCTGCCTCTGAATTTGACCGACCTCTTAGACCC ATTGTTGTCAAGGATTCTGAGGGAACCGTGAGCTTTCTCTCTGACTCAGGAAAAAAGGAACAGATGCCAC TAACCTCTCCAAGATTTGATAGTGATGAAGGGGACCAGTGCTCAAGACTCTTGGAACTAGTAAAAGATAT TTCCAGTCATTTGGATGTCACAGCCTTGTGTCACAAAATTTTCTTGCATATCCATGGACTTATCTCTGCT GATCGCTATTCCCTGTTCCTTGTCTGTGAGGACAGCTCTAAAGACAAATTTCTCGTCAGTCGCCTCTTTG ATGTTGCTGAAGGTTCAACACTGGAGGAAGCTTCAAATAACTGTATCCGTTTAGAATGGAACAAAGGCAT TGTGGGACATGTGGCAGCTTTTGGAGAGCCCTTGAACATCAAAGATGCCTATGAGGATCCCCGGTTCAAT GCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCTTTGTATGCCAATTAAGAACCACAGGG AAGAGGTCGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTCACTGAAAAAGA TGAAAAGGACTTTGCTGCCTATCTGGCATTTTGTGGCATTGTTCTTCACAACGCCCAGCTCTATGAAACG TCACTGCTGGAGAACAAAAGAAATCAGGTATTACTTGACCTTGCTAGCTTAATTTTCGAGGAGCAGCAGT CATTGGAAGTAATTCTGAAGAAAATAGCCGCCACTATTATCTCCTTCATGCAGGTGCAGAAGTGCACCAT CTTCATCGTGGATGAAGACTGCCCTGACTCGTTCTCTCGTGTGTTTCAAATGGAGTGGGAGGAAGTAGGG AAATCCTCCGAGCCTTTGACAAGGGAGCACGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCA AAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGACAACAGATTTCCCTGGACAAATGAAAA CATGGGCCATATAAACACGCACTGCATCAGAAGTTTGCTTTGCACGCCTATAAAAAATGGGAAGAAGAAC AAAGTCATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAAGACTGGCAAGATTAAGGCTTTCAACC AAAACGATGAACAATTCCTGGAGGCTTTTGTCATCTTCTGTGGCTTGGGGATCCAGAACACACAGATGTA CGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACGTTAGAGGTCCTGTCTTATCACGCATCAGCG GCGGAGGAAGAAACAAGGGAGCTCCAGGCTCTAGCGGCTGCTGTGGTACCGTCTGCCCAAACCCTTAAAA TTACCGACTTCAGCTTCAGTGACTTCGAGCTGTCTGATCTGGAAACAGCGCTGTGTACAATTCGGATGTT CACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACACGAGGTTCTTTGCCGATGGATTTTGAGTGTC AAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCACGCCTTTAACACGGCACAGTGCATGT TTGCTGCTCTGAAAGCAGGCAAGATTCAGAACAAGTTGACGGATCTGGAGACGCTTGCATTGCTGATTGC CGCTTTAAGCCATGATTTGGACCATCGTGGTGTGAACAACTCATACATACAGCGGAGCGAGCACCCCCTG GCCCAGCTGTACTGCCACTCCACCATGGAGCACCATCACTTCGACCAGTGCTTGATGGTTCTAAACAGCC CAGGCAACCAGATCCTCAGTGGCCTCTCCATTGAAGAATATAAGACCACATTGAAAATAATCAAGCAAGC AATTTTAGCCACTGACCTAGCACTGTACATTAAGAGACGAGGAGAATTTTTTGAACTTATAAGAAAAAAT GAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCTGGCAATGCTGATGACTGCCTGTGATCTCT CCGCCATTACTAAGCCCTGGCCTATTCAACAACGGATAGCAGAACTCGTGGCAGCCGAATTCTTTGATCA AGGAGACAGAGAGAGAAAAGAACTTAACATGGAGCCCGCTGATCTAATGAACAGGGAGAAGAAAAATAAA ATCCCAAGTATGCAAGTTGGGTTCATAGACGCCATCTGCTTGCAGCTGTATGAGGCCCTGACCCACGTCT CAGAAGACTGTTTGCCCCTGCTGGATGGCTGCAGGAAGAACAGGCAGAAATGGCAAGCCCTCGCAGACCA GCAGGAGAAGACGCTCCTTAATGGGGAGAGCGGCCAGGCCAAGCGGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133584 |
| Insert Size | 2502 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133584.1, NP_598268.1 |
| RefSeq Size | 3397 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 171115 |
| UniProt ID | O54735 |
| Gene Summary | catalyzes the hydrolysis of cGMP to 5'-GMP; may regulate vascular relaxation; may be involved in establishment of cerebellar neural networks [RGD, Feb 2006] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR206532 | Pde5a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 5A, cGMP-specific (Pde5a), (10 ug) |
CNY 10,640.00 |
|
| RR206532L3 | Lenti ORF clone of Pde5a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 5A, cGMP-specific (Pde5a), (10 ug) |
CNY 12,540.00 |
|
| RR206532L4 | Lenti ORF clone of Pde5a (mGFP-tagged ORF) - Rat phosphodiesterase 5A, cGMP-specific (Pde5a), (10 ug) |
CNY 13,680.00 |
