C4a (NM_031504) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	C4; C4-1; C4b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206490 representing NM_031504 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCCTCTGGGGCCTGGCCTGGGCATTCAGCTTCTTCGCCTCCTCTCTGCAGAAGCCCAGGTTGC TCCTGTTCTCCCCTTCTGTGGTTAATTTGGGGACCCCCCTGTCAGTAGGGGTGCAGCTCCTGGATGCCCC TGCAGGACAGGAGGTGAAAGGCTCAGTGTACCTCAGAAACCCAACAAGCGGTCCCTGCTCCCCAAAGAAG GACTTTAAGCTCAGCTCGGGAAATGACTTTGTGCTTCTCAGACTTGAGGTCCCACTGAAAGATGTGAGGA GTTGTGGCCTCTTTGGCCTGCGCAGAGCCCCCCACATCCAGCTGGTGGCTCACTCTCCGTGGCTAAAGAA CACAGCATCCAAAGCCACCGAGACTCAGGGGGTCAACCTGCTTTTCTCTTCCCGACGAGGCCACATCTTT GTGCAGACCGATCAGCCTATCTATAACCCTGGGCAGCGGGTTCGTTATCGGGTCTTTGCACTGGATCAAA AGATGCGTCCATCTACTGACACCCTCACGGTCACAGTGGAGAACTCCCATGGCCTCCGTGTACGCAAGAA GGAGGTATTTGCCCCCACATCCATCTTCCAAGATGACTTCATAATTCCAGACATCTCAGAGCCGGGGACC TGGAAGATCTCAGCTAGGTTCTCAGATGGCCTCGAGTCCAACAGAAGCACCCACTTCGAAGTGAAGAAAT ATGTCCTTCCCAACTTTGAGGTGAAGCTTACTCCTTGGAAGCCATATATCCTGACAGTGCCTAGCTACCG TGAAGAAATACAATTAGACGTCCAGGCCAGATACGTCTACGGGAAGCCCGTGCAGGGCGTGGCATATACC CGCTTCGCACTCATGGATGAGCAGGGGAAGAAGTCTTTCCTCCGGGGCCTAGAGACCCAGACCAAGTTGG TGGAAGGCCAGACTCACATCTCCATCTCAAGGGACCAGTTCCAGGCTGCCCTGGGTAAAGTGAATACTGA GATCGGAGACCTGGAGGGGCTGCGGCTCTACGCTGCCGTAGCTGTCATTGAGTCTCCAGGAGGAGAGATG GAGGAGGCGGAGCTCACATCCTGGCCCTTCGTGTCATCTGCCTTCTCCTTGGATCTCAGCCACACCAAGC AGCATCTTGTGCCCGGGGCCCCCTTCCTGCTGCAGGCCCTGGTTCGAGAAATGTCAGGCTCTGAAGCCTC TGATGTTCCTGTCAAAGTCTCTGCCACATTGTTGTCTGGGTCCGACTCGAAAGTCCTTGACTTTCAACAG AACACCAATGGGATTGGCCAAGTCAGCTTTTCCATCCATGTCCCGCCAACCATCACCGAACTTCGACTCT TGGTATCTGCCGGTTCCCTCTACCCAGCTGTAGCCAAGCTCACCGTGCAAGCCCCACCTTCAAGAGGCCC CGGCTTTCTGTCCATTGAGCCATTAGACCTTCGGTCCCCTCGTGTGGGGGACACCTTCGTCCTGAGCCTT CGAACCGTGGGCATCCCTATGCCTACCTTCTCTCATTACTACTACATGATCATCTCCAGAGGCCAGATCA TGGCCATGAGTCGGGAGCCCAGGAGGGCCCTGACCTCCATCTCTGTCTTGGTGGACCATCACCTGGCTCC CTCTTTCTACTTTGTGGCCTACTTCTATCACCAAGGACTCCCAGTGGCCAACTCCCTGCTCATCAACGTC CAGCCCGGGGACTGTGAGGGCAAGCTGGAATTGAAGGTGGATGGTGCCAAGGAGTATCGCAATGGAGACA GCATGAAGCTCCAACTTCAAACTGACTCTGAAGCCCTGGTGGCACTGGGAGCTGTGGACACAGCTCTGTA TGCTGTGGGTGGCCGGTCTCACAAACCCCTCGACATGAGCAAGGTCTTTGAAGTAATGAACAGCTACAAC CTTGGCTGTGGTCCCGGAGGCGGGGACGATGCCCTTCAGGTGTTCCAGACTGCTGGTCTGGCCTTTTCTG ACGGTGATCGACTAACTCAGACCAAAGAGAACCTGAGCTGTCCCAAGGAGAAGAAAAGTCGGCAAAAGCG AAATGTTAACTTCCAGAAGGCTATTAGCGAGAAGTTGGGCCAGTATTCCTCTCCAGACACCAAGCGCTGC TGCCAGGATGGGATGACCAAGCTGCCCATGGCACGCACCTGCGAGCAGAGGGCAGCCCGCGTGCCTCAGC CAGCCTGCCGGGAGCCCTTCTTGTCCTGTTGCAAGTTTGCTGAGGACCTCCGCAGGAACCAGACCAGGAG CCAGGCGGGCCTCGCCCGAGCCCAGGACATGCTGCAGGAGGAGGATCTGATAGATGAAGATGACATTCTC GTGCGCAGCTTCTTCCCAGAGAACTGGCTCTGGAGAGTGGAACCTGTGGACCGCTCCAAACTGTTGACTG TGTGGCTCCCTGACTCTCTGACCACATGGGAGATTCATGGTGTGAGCCTGTCCAAAAGCAAAGGCTTGTG TGTAGCCAAGCCAACTCGCGTGCGAGTGTTCCGAGAATTCCACCTGCACCTGCGCCTGCCCATCTCTGTC CGGCGCTTCGAGCAGCTTGAATTACGGCCCGTTCTCTACAACTATCTGAGTGAGGACGTGACTGTAAGTG TCCATGTGTCCCCAGTGGAGGGGCTGTGCCTGGCTGGTGGTGGATTGCTGGCCCAGCAAGTATCAGTGCC TGCAGGCTCCGCCCGGCCTGTGGCCTTCTCTGTGGTACCCACAGCTGCTGCCAGCATACCCCTGAAAGTG GTGGCTCGGGGGTCTTTTACTATAGGGGATGCTGTGTCTAAGATTCTCCAAATTGAGAAGGAAGGGGCCA TCCACAGAGAAGAGATAGTCTACAACCTCGACCCCCTAAATAACCTTGGTCGGAGTTTGGAAATCCCTGG CAGCTCAGACCCCAACGTCATTCCTGATGGAGATTTCAGCAGCTTCGTCAGGGTTACAGCCTCCGAACCC TTGGAGACTTTGGGCTCCGAAGGCGCCTTGTCCCCAGGCGGAGTGGCCTCCCTCCTGAGGCTCCCTAGAG GCTGTGCAGAACAAACCATGATCTATTTGGCCCCTACCCTGACTGCTTCCCACTACCTGGACAGGACGGA GCAGTGGAGCAAACTGCCCCCTGAGACAAAGGACCATGCTGTGGATCTGATTCAGAAAGGCCACATGCGG ATCCAGCAGTTTCGGAAGAAAGATGGCTCCTTTGGGGCATGGTTACACCGGGACAGCAGCACCTGGCTCA CGGCTTTTGTGCTGAAGATTCTGAGTCTGGCCCAGGAACAGATTGGTGACTCCCCCGAGAAGCTGCAAGA GACCGCTGGGTGGCTGCTGGGCCAGCAGCTGGACGATGGCTCATTCCACGACCCGTGTCCAGTCATCCAC AGAGGCATGCAGGGGGGCTTGGTGGGAACCGATGAGACCGTGGCACTGACCGCCTTTGTGGTCATCGCCC TTCACCACGGGCTGGCCGTCTTCCAGGACGAGAACTCACAGCAGCTGAAGAAGAGGGTGGAAGCCTCCAT CACGAAGGCGAACTCGTTCTTGGGGCAGAAGGCAAGTGCTGGGCTCCTGGGTGCACACGCATCCGCCATC ACAGCCTATGCCCTGACACTGACCAAGGCCTCGGAGGACCTGCGGAATGTTGCCCACAACAGCCTGATGG CCATGGCTGAGGAAACAGGGGAAAACCTCTACTGGGGCTCAGCCATTGGCTCTCAGGACAACGTTGTGTC GTCCACCCCAGCCCCTCGTAATCCCTCAGAACCTGTGCCCCAGGCCCCAGCCCTGTGGATCGAAACCACA GCCTACGGCCTGCTACATCTGCTGCTGCGGGAGGGGAAGGGAGAAATGGCTGACAAGGTGGCAACCTGGC TCACACACCAGGGAAGCTTCCAAGGGGGATTCCGCAGTACCCAGGACACTGTGGTCACCCTGGATGCCCT GTCTGCATACTGGATCGCTTCACACACCACCGAGGAGAAGGCACTCAATGTGACTCTCAGCTCCATGGGC CGCAATGGGTACAAGTCCCACCTGCTACAGCTGAACAACCACCAAGTCAAGGGCCTGGAGGAGGAGCTGA AGTTCTCCCTGGGCAGCACCATCAATGTGAAAGTGGGAGGAAACAGCAAAGGCACCTTAAAGATCCTTCG TACCTACAACGTCCTGGACATGAAGAACACAACATGCCAAGACCTTCGGATAGAAGTGACGGTCACAGGC TATGTGGAATATACAAGGGAGGCCAACGAAGACTATGAATACGACTATGATATGCCAGCCGCAGATGACC CCAGTGTCCACTCGCAGCCTGTGACACCCCTGCAGCTGTTTGAGGGTCGTCGGAGCCGCCGCAGGAGGGA GGCCCCCAAGGCTGCGGATGAGCAGGAGTCCAGAGTGCAGTACACCGTGTGCATCTGGCGGAACGGTAAC CTGGGGCTGTCTGGGATGGCCATTGCTGACATCACCCTCCTGAGTGGATTCCAAGCCCTGAGGGCCGACC TGGAGAAGCTGACCTCCCTCTCTGACCGTTACGTGAGTCACTTTGAGACCGACGGGCCCCACGTCCTACT ATACTTTGACTCGGTCCCGACCACCCGGGAGTGTGTGGGCTTTGGAGCCTTGCAGGAGGTGGCCGTGGGG CTGGTGCAGCCAGCCAGTGCTGTCCTATATGACTACTACAGCCCTGATCACAAGTGCTCTGTGTTCTATG CTGCACCCACCAAGAGCAAGCTCCTCTCCACCTTGTGCTCTGCAGACGTCTGCCAGTGCGCTGAAGGGAA GTGTCCTCGACAGCGAAGGTCACTGGAGCGAGGGGTGGAGGACAAGGATGGTTACCGGATGAAGTTCGCC TGCTATTATCCCCGAGTGGAGTATGGCTTCCAGGTTAAGGTTCTCCGAGAAGACAGCAGAGCTGCCTTCC GCCTCTTTGAGACCAAGATCACCCAAGTCCTGCATTTCACAAAGGATGCCAAGGCCTCCATAGGTCAGAC CCGCAACTTTCTGGTCCGGGCCTCTTGCCGCCTTCGTTTGGAGCCTAGCAAAGAGTACTTAATCATGGGG ATGGACGGAGTCACCAGTGACCTCAAGGGAGACCCCCAGTACTTGCTGGACTCAAATACTTGGATCGAGG AGATGCCATCTGAGCGCCTGTGCAGGAGCACCCGTCAGCGGGCAGCCTGTGGCCAGCTCAATGACTTTCT GCAGGAGTACAGCAGCCAGGGGTGCCAGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031504
Insert Size	5214 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031504.3, NP_113692.2
RefSeq Size	5377 bp
RefSeq ORF	5214 bp
Locus ID	24233
UniProt ID	P08649
Gene Summary	complement protein, part of the classical activation pathway; localizes to the class III region of the MHC [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.