Twf1 (NM_001008521) Rat Untagged Clone
CAT#: RN206444
Twf1 (untagged ORF) - Rat twinfilin, actin-binding protein, homolog 1 (Drosophila) (Twf1), (10 ug)
CNY 3,990.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Ptk9 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN206444 representing NM_001008521
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCACCAGACCGGCATCCAAGCAAGTGAAGACGTCAAAGAGATCTTTGCCAGAGCCAGAAATGGGA AATACCGACTTCTAAAAATATCGATTGAAAATGAGCAACTGGTGGTCGGGTCCTGTAGTCCGCCTTCAGA TTCCTGGGAACAGGATTACGATCCCTTTGTCTTGCCCCTGCTGGAGGACAAGCAACCGTGCTATGTGTTA TTCAGGTTAGATTCTCAGAATGCCCAGGGATATGAGTGGATATTCATTGCATGGTCTCCAGATCATTCTC ATGTTCGTCAAAAAATGCTGTATGCGGCAACAAGAGCAACTCTGAAAAAAGAATTTGGAGGTGGCCACAT TAAAGATGAAGTGTTTGGAACAGTAAAGGAGGACGTATCACTACATGGATATAGAAAGTATCTTCTCTCC CAGTCTTCTCCTGCCCCTCTGACTGCAGCAGAGGAAGAATTAAGGCAGATTAAAATCAGTGAGGTACAAA CTGACGTGAGTGTGGACACAAAGCATCAGACACTACAAGGAGTAGCATTTCCTATTTCTCGAGATGCCTT CCAGGCTTTGGAAAAACTGAGTAAGAGACAGCTCAACTATGTGCAGTTGGAAATAGATATAAAAAATGAA ACAATAATTTTGGCCAACACAGAAAATACAGAACTAAAAGATTTGCCAAAGAGGATTCCCAAGGATTCAG CACGCTACCATTTCTTTCTGTATAAGCATTCCCACGAAGGAGACTACTTAGAGTCCATAGTTTTTATTTA TTCAATGCCCGGATACACATGCAGCATAAGAGAGCGGATGCTGTATTCCAGCTGCAAGAGCCCTCTGTTA GATATTGTAGAAAGACAACTGCAGATGGATGTGATCAGAAAGATTGAGATAGACAATGGGGATGAACTGA CCGCAGATTTCCTGTACGATGAAGTCCATCCCAAGCAGCATGCACATAAGCAGAGTTTTGCTAAACCAAA GGGTCCTGCGGGAAAGAGAGGGATTAGAAGATTGATTAGGGGTCCAGCGGAAGCAGAAGCCACTACTGAT TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001008521 |
Insert Size | 1053 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001008521.1, NP_001008521.1 |
RefSeq Size | 2895 bp |
RefSeq ORF | 1053 bp |
Locus ID | 315265 |
UniProt ID | Q5RJR2 |
Gene Summary | Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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MicroRNA-206 inhibits stemness and metastasis of breast cancer by targeting MKL1/IL11 pathway
,null,
Clinical cancer research : an official journal of the American Association for Cancer Research
,PubMed ID 27435395
[Twf1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR206444 | Twf1 (Myc-DDK-tagged ORF) - Rat twinfilin, actin-binding protein, homolog 1 (Drosophila) (Twf1), (10 ug) |
CNY 3,990.00 |
|
RR206444L3 | Lenti ORF clone of Twf1 (Myc-DDK-tagged ORF) - Rat twinfilin, actin-binding protein, homolog 1 (Drosophila) (Twf1), (10 ug) |
CNY 6,080.00 |
|
RR206444L4 | Lenti ORF clone of Twf1 (mGFP-tagged ORF) - Rat twinfilin, actin-binding protein, homolog 1 (Drosophila) (Twf1), (10 ug) |
CNY 6,650.00 |