U2af1l4 (NM_001008775) Rat Untagged Clone

CAT#: RN206421

U2af1l4 (untagged ORF) - Rat U2 small nuclear RNA auxiliary factor 1-like 4 (U2af1l4), (10 ug)



  "NM_001008775" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206421 representing NM_001008775
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAATATTTAGCTTCGATATTCGGGACTGAGAAGGACAAGGTTAACTGCTCTTTTTACTTTAAGA
TTGGGGCCTGCCGGCACGGGGACCGGTGCTCCCGACTTCACAACAAACCGACTTTCAGCCAGACCATAGT
CCTGCTCAACTTGTACCGGAACCCACAGAACACAGCCCAAACTGCAGATGGATCACACTGTCACGTGAGC
GATGTGGAGGTGCAAGAACACTATGATAACTTCTTTGAGGAGGTATTCACAGAACTGCAGGAGAAGTATG
GAGAAATTGAAGAGATGAATGTGTGTGATAACCTCGGGGACCACCTCGTGGGCAATGTCTATGTTAAGTT
CCGGCGGGAGGAGGATGCAGAGCGGGCTGTGGCGGAACTCAATAACCGCTGGTTCAACGGGCAGGCTGTG
CATGCTGAGCTGTCTCCGGTCACTGACTTCCGAGAGTCATGCTGCCGACAGTATGAGATGGGGGAATGTA
CCCGAGGTGGCTTCTGCAACTTTATGCACCTGCGACCCATATCCAGGAACTTGCGCCGGCAGCTCTATGG
GCGAGGACCCAGGCATAGGTCACCTCCAAGGTCCCATACAGGTCACCGACCCCGAGAAAGGAACCGACGC
CGTTCCCCAGACCACCGGCATGGTCGCTTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001008775
Insert Size 663 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001008775.1, NP_001008775.1
RefSeq Size 709 bp
RefSeq ORF 663 bp
Locus ID 361542
UniProt ID Q7TP17
Gene Summary RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site. Shows a preference for AGC or AGA.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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