Top1 (NM_022615) Rat Untagged Clone

CAT#: RN206407

Top1 (untagged ORF) - Rat topoisomerase (DNA) I (Top1), (10 ug)



  "NM_022615" in other vectors (3)

CNY 9,880.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Ab2-086
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206407 representing NM_022615
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGGGACCATCTCCACAACGACTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCATAAAC
ACAAAGATAAACACAAAGATCGAGAACACCGGCATAAGGAGCACAAGAAGGATAAGGATAAGGACCGGGA
AAAGTCTAAACATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAAGAGAAAACCAAA
CACAAAGATGGAAGCTCAGACAAACATAAAGACAAACATAAAGACAGAGACAAGGAAAAACGAAAGGAGG
AAAAGATTAGAGCTGCTGGGGATGCAAAAATAAAGAAGGAGAAGGAAAATGGCTTCTCTAGTCCACCACG
AATTAAAGACGAGCCTGAAGATGATGGCTATTTTGCTCCTCCAAAAGAGGATATAAAGCCACTGAAGAGG
CCTCGGGATGAAGATGATGCTGATTATAAGCCTAAGAAAATTAAGACAGAAGATATCAAGAAGGAGAAGA
AACGAAAACTAGAAGAAGAAGAGGATGGCAAACTTAAAAAGCCCAAGAATAAAGATAAAGATAAAAAGGT
TGCTGAGCCAGATAATAAGAAGAAGAAGGCGAAAAAGGAAGAGGAGCAGAAGTGGAAATGGTGGGAAGAA
GAACGCTATCCAGAAGGCATCAAGTGGAAATTCCTAGAACATAAAGGGCCTGTTTTCGCTCCACCGTATG
AGCCTCTGCCAGAGGGTGTCAAGTTTTACTATGATGGTAAAGTTATGAAGCTGAGTCCCAAAGCAGAAGA
AGTAGCTACATTCTTTGCAAAAATGCTTGACCATGAATATACTACTAAGGAAATATTCAGGAAAAATTTC
TTTAAAGACTGGAGAAAGGAAATGACTAATGATGAAAAAAATACGATTACCAACCTAAGCAAATGCGACT
TTACACAGATGAGCCAGTACTTCAAAGCTCAATCAGAAGCTCGGAAACAGATGAGCAAGGAAGAAAAATT
GAAAATCAAAGAAGAAAATGAAAAATTATTGAAAGAATATGGCTTTTGTGTAATGGATAATCATAGAGAG
CGGATTGCCAACTTCAAGATAGAGCCTCCAGGGCTCTTCCGAGGCCGTGGCAACCACCCCAAGATGGGTA
TGCTGAAGAGAAGGATCATGCCTGAGGACATCATCATCAACTGTAGCAAAGATGCCAAGGTTCCTTCTCC
TCCTCCTGGACATAAATGGAAGGAAGTTCGACATGATAACAAGGTTACTTGGCTGGTCTCCTGGACAGAG
AATATCCAAGGTTCTATCAAATATATCATGCTGAATCCCAGTTCACGAATCAAGGGTGAGAAAGACTGGC
AGAAGTATGAGACTGCTCGGCGACTGAAGAAGTGTGTGGACAAGATCCGAAATCAGTATCGGGAAGACTG
GAAGTCCAAAGAAATGAAAGTTCGGCAGAGAGCTGTAGCACTGTACTTCATTGATAAGCTTGCTCTGAGA
GCAGGCAATGAGAAGGAAGAAGGAGAGACAGCAGACACTGTGGGTTGCTGTTCACTTAGAGTGGAACACA
TCAATCTACACCCAGAATTGGATGGTCAGGAGTACGTGGTGGAGTTTGACTTTCCTGGGAAGGACTCAAT
CAGATACTACAACAAAGTCCCAGTTGAGAAACGAGTTTTTAAAAACTTACAACTATTTATGGAGAACAAG
CAGCCTGAGGATGATCTTTTTGATCGACTTAATACTGGTATTCTAAATAAGCATCTTCAAGATCTCATGG
AGGGCTTGACTGCCAAGGTGTTCCGTACCTACAATGCCTCCATCACGCTGCAGCAGCAGCTTAAAGAGCT
CACAGCCCCTGATGAGAATGTACCAGCAAAGATTCTATCTTATAACCGTGCCAATCGCGCTGTTGCAATT
CTTTGTAACCACCAGAGGGCGCCACCAAAGACCTTTGAGAAGTCAATGATGAACTTGCAGTCTAAGATTG
ATGCCAAGAAAGATCAGCTAGCAGATGCTCGAAAGGACCTGAAAAGCGCTAAGGCTGATGCCAAAGTCAT
GAAGGATGCAAAGACCAAGAAGGTAGTAGAGTCAAAAAAGAAGGCTGTACAGAGATTAGAAGAGCAGCTG
ATGAAGCTGGAGGTTCAAGCCACAGACCGAGAGGAGAACAAACAAATTGCCTTGGGGACCTCCAAACTCA
ATTACCTGGACCCTAGGATCACAGTGGCTTGGTGCAAAAAATGGGGGGTCCCAATCGAGAAGATTTACAA
CAAAACCCAGAGAGAGAAGTTTGCTTGGGCCATTGATATGACTGATGAGGACTACGAGTTTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_022615
Insert Size 2304 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022615.1, NP_072137.1
RefSeq Size 2304 bp
RefSeq ORF 2304 bp
Locus ID 64550
UniProt ID Q9WUL0
Gene Summary human homolog catalyzes the transient breakage and rejoining of DNA; plays a role in nucleotide excision repair [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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