Plb1 (NM_138898) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Phlpb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206357 representing NM_138898 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCATGGCCAGGCGTTTCCCTGGTGGGACTGCTGCTGCTGCTACTGCTGGGACAAGGGCCCTCCC AAATCCACGGCTCTTCTGGAGAAAACACATCGCAGCCCCAGCAAGTGTTTCGGACCCTGAAGAATTTTTC ATTCCCTTGCAAGCCAAAGAAGTTAGAACTGAGTGTGCTTTCTAAGTCAGTTCACTCTCTGAGACCCTCA GACATTAAACTCGTGGCAGCCATCGGCAACCTAGAAACTCCTCCAGCCCCTGGCTCAGGCGTGGTCAACA TGGAGAAACCTCAAAGCCTCGAGAGCGAACTACAGAATGTGTGCATAGGAATCATGACAGCCCTTTCAGA TATCATCAGACATTTCAACCCTTCTGTTCTGATGCCCACGTGTTCTCCTGGGAAGGGTACTGCAGGCCAC ACTACTATTGCCGAAGACTTGTGGATTCAGGCTAAAGAGCTGGTGAGGCATCTGAAAGACAACCCGGAAC TTGATTTTGAGAAGGACTGGAAACTCATCACTGTGCTCTTCAGTAACACAAGCCAGTGCCACCTGTGTTC CTCTGATCAGCAGAAAAGGCACTTGATGAAGCACATGGAGATGCTGTCAGGGGTGCTGGATTACCTGCAT CGTGAGGTCCCCAGAGCGTTTGTGAATTTGGTGGATCTCTCTGAGGTTTTGACCATGGCTCAGCAGCATC AAGAGACTGGTTTCAGCCCTGCACCAGAGATTTGCAAATGCTCAGAGGAAATAACGAAGTTATCCAAGGC TGTCATGCAGTGGTCCTATCAGGAAGCCTGGGAAGATCTCCTGGCCTCCAGCAAGTTCAATAAGCATGAG ACCTTCGCTGTGGTTTTCCAGTCTTTCTTCTCTGAGGTAGAACTACCCTTGGAGAGGCCCTCGCCCCAGG ATTCCACCACGCTCGCCCTCAGGATCTGGAATAGCATGATGGAACCAGTGGGTCGAAAGGATGGGACACT CAATGAAGCAGAGAGAAAAACAATGAAATGTCCCTCTCAGGAGAGCCCCTATCTGTTCACCTACAGAAAT AGCAACTACCAGGCCAGACAGCTGAAACCCATAGGAAAGTTTCAGATGAAAGAAGGAACAAAATTCACCT GTCCTGACAAAGACCCCTCGGACTCCATCCCCACAACAGTTCACAGGCTGAGGCCGGCTGACATCAAGGT CATCGGAGCCATGGGTGACTCGCTCACGGCAGGCAACGGGGCAGGGTCCAGCCCTGGGAATGTCTTGGAT GTCTTAACTCAATACCGAGGCCTGTCGTGGAGTGTGGGCGGAGATGAGACCATCGAGACCGTGACCACCC TAGCCAACATCCTCCGGGAATTCAACCCCTCCTTGAAGGGCTTCTCTGTTGGCACTGGGAAAGAAAACAC TCCCCGAGCATCCTTCAACCAGGCCGTAGCAGGAGCCAAATCTGATGGCTTAGCTGCCCAGGCCAAAAAG CTGGTGAGCCTGATGAAGGATGACAAGACAATAAACTTTCAGGAAGACTGGAAGATAATCACTGTGTTTA TAGGAGGCAACGACCTCTGTGGCTCCTGCAATAACCTGGCTCGCTTTTCTCCCCAAACCTTCACAGACAA CATCAAGACGGCCCTGGACATCCTCCATGCAGAGGTTCCCCGGGCCTTTGTGAACATGGTCTCGGTGATT GAGATCACCCCCTTGAGAGAACTGTTCAATGAACCTAAAGTCAGCTGCCCACGGATGATCCTCAGGAGCC TGTGTCCTTGTGTCTTGAACCTTGGTGAGAACTCAGCAGAACTTGCCCAACTTGTGGAAAGGAACAGGCA GTATCAGGAAGAAACTGGAAAACTGATTGAGAGTGGCCGATACGACACCAGGGATGACTTCACCGTGGTC CTCCAGCCCATGTTTGAAAATGTCGTCATGCCACGGACCCTGGAGGGCTTGCCCGACAGCTCTTTCTTTG CCCCTGACTGTTTCCACTTCAATGTCAAGACTCACGCTCGCTCAGCCATCGCCCTCTGGAAGAACATGCT AGAACCTGTGGGCCGCAAGACAAGACATCAGAATTTTGAAATCAAGGTCCCTATCATGTGTCCAAACCAG ACCTCACCGTTTCTGAGCACCACCAAGAACAGCAACCTGGGACATGGAACTTCGATGTCTTGTGAGGAGA AAGCCCCCTCTGCCTCACCACCAACCTCAGTGCATACCCTGAGACCTGCAGACATCCAAGTTGTGGCAGC TCTGGGAGACTCTGTGACTGCTGGCAATGGAATCAGCTCCCAAGAAGGTGATCTCGCTGATGTTACCACA CAGTATCGAGGACTGTCCTACAGTGCTGGTGGGGACAAGTTCCTGGAGAATGTGACCACCTTGCCCAACA TCCTCCGGGAATTTAATGGAAATCTCACAGGCTACTCAGTCGGAACCGGTGACGTCAACTCTGCAAGCGC GTTCCTTAACCAGGCTGTCCCTGGGGCAAAGGCTGAGAACCTTGCAAGTCAAGTCCAGACTCTGATTCAG AAGATGAAGAATGACACCAGAGTGAACTTTCACCAAGACTGGAAGGTCATCACTGTGATGATTGGGGCCA GCGACTTGTGTGACTTCTGCAAGGATTCGAATCGTTACTCTGCAGCCAATTTTTCTGACCATCTCCGCAA TGCCTTGGACATCCTGCATAAGGAGGTACCCAGAGCCCTGGTCAACCTTGTGGACTTCATGAACCCCAGT ATCATTCGGCAAGTGTTCCTGAAGAACCCAGACAAGTGCCCTGTGAATCAGACCAGTGTCCTGTGCAACT GTGTTCTGACCCCAGGGGAGGATTCCCATGAGCTGGCAAGGTTGGAGGCCTTCACCAAATCCTACCAGAG TAGCATGCTTCAACTGGTTGAGTCAGGCCGCTACGACACCCGGGAGGATTTCTCTGTGGTACTGCAGCCC TTTCTCTTCAACATCAGGCTCCCCATCCTAGAGAATGGGAATCCAGATACATCCTTCTTTGCCCCAGACT GCATCCTCCTAAGCCAGAAGTTCCACACTCAGCTCGCGAGAGCCCTTTGGGCCAATATGCTTGAACCCCT GGGAAAGAAAATGGATACTTTGGACCCGAAAGAACTCATAGCTTTGGCCTGCCCCACCAAGGACAAGCCC TTCCTGAGAACCTTCCGGAACAGTAACTACACGTACCCTATCAAGCCAGCCATTGAGAATTGGGGCAGTG ACTTCCTGTGCACAGAGCAGAGTCCTTCCAGCAAGGTACCCACCTCAGTTCATGAGCTCCGACCATCAGA CATCAAGGTGGTGGCAGCAATGGGTGACTTTCTGACGACAGCCACCGGAGCTCGACCAAGTGAGTCCAGC AGTCTAGACACCCCCTGGAGGGGGCTGTCTTGGAGCATTGGAGGAGATGGAACGTTGGAGACCCATACCA CACTGCCCAACATCCTGAAGAAGTTCAACCCTTCCATCCTTGGATTCTCCACCGGTACCCTGGAGAACAC GGCAGGATTAAATGTGGCAGAAGAAGGCGCCAGAGCTCAGGACATGCCGGCCCAGGCTCAGGCCCTGGTG AAGAAGATGAAAAGCACCCCTACAATCAACATACAGGAAGACTGGAAGCTGATTACACTCCTCATCGGGA ACAACGACCTGTGTCTTTACTGTGAGGATCCGGAGAACTACTCAACCAGGGAGTATGTCAAGTACATCCA GCATGCCTTGGACATCTTCTATGAGGAGCTTCCCAGGGTTTTCATCAACGTGGTGGAAGTCATGGAGCTG TCCGGTCTGCTCCACGACCAGGGCGGGAAATGTGCCATGCCGTTGGCTGTCCAGAAAAACTGCAGTTGCC TTAAACGCTCTCAAAACCTCATGGCAATGCAGGAGCTGAAGAAAGTCAACGGGAACCTCCAGAGTGCCCT CTCGGAGCTCTCCTACTGGCATCGGTACATGCAGCGTGAGGACTTTGCAGTCACTGTCCAGCCTTTCTTC CGGAATACCTTTGTCCCACTGGATGAGCGTGGGGGCCTCGACCTCACTTTCTTCTCTGAAGACTGTTTCC ACTTCTCAGTCCGTGGGCATGCTGAGATGGCCATTGCCCTCTGGAACAACATGCTGGAACCAGTGGGCAA GAAGACAACCTCCAATAACTTCACATACAACCGGACCAAACTCAAGTGTCCCTCGCCTGAAAACCCTTTC CTCTACACTGTCCGGAACAGTCAGATTCTTCTAGACAAGGCTAAAGAAAACTCCAATACACTCTACTGGG CAGTGCCAGTGGCTGCAGTAGGTGGCCTGGTAGTTGGCATCCTTGGAATGATGTTGTGGAGAACTGTGAG ACTCGTCCAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_138898
Insert Size	4353 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138898.1, NP_620253.1
RefSeq Size	4590 bp
RefSeq ORF	4353 bp
Locus ID	192259
UniProt ID	O54728
Gene Summary	exhibits broad lipolytic activities (PLA2, lysophospholipase, and lipase activities); may mediate terminal digestion, or membrane digestion, of dietary lipids and biliary phospholipids [RGD, Feb 2006]

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