Depdc5 (NM_001107229) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206263 representing NM_001107229 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGACTAAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACCCAGAAAGTGGAATTTGCTGGCA TCAGGGCACAGGCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGAC CAGGGTGGTGTTCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGAT TTTGATATTTATGGGGATCTGTACTTTGAGAAAGCTGTGAATGGTTTCCTTGCCGACCTGTTTACTAAGT GGAAGGAGAAGAACTGTAGCCATGAAGTGACTGTGGTTCTGTTTTCTAGAACTTTTTATGATGCAAAATC TATTGATGAATTTCCTGAAGTAAACCGAGCTTCAATTCAACAGGATCATAAAGGGAGATTCTACGAGGAC TTTTACAAAGTGGTGGTGCAGAACGAGAGAAGGGAAGAGTGGACGTCACTCCTTGTGACCATTAAGAAAC TCTTCATCCAGTATCCAGTGTTGGTTCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTAC CTCAGCACAAGGAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAAC CGAAACTTTGACCGAACTGGGCAGATGTCCGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACC GCCTCCTCATGATCTTGACCAAGCAGCGGATGATCGATAATGGAATTGGTGTGGACTTAGTGTGCATGGG AGAGCAGCCATTACACGCTGTGCCATTATTCAAGCTGCACAACCGGAGTGTGCCACGGGATTCTCGGCTG GGCGATGACTATAATATTCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTGTTTTGTA ACAGTTTCACTCCAAGGATAAAACTGGCAGGAAAGAAGTCTGCCACTGAGAAAACCAAAAATGGCCGTGA TACATCTCTTGGGGCTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGAT GCCCAAGTATTCCGGCTGCCTGGTCCATCCCGGGCCCAGCGACTCGCCACCTGCAGGTCTGTGAGAGAGC AGGAAAATCACAATCGCAAAAGTGCCAGCTCCTGTGACGTCTCCTCCAGCCCTTCCTTGCCAAGCCGTGC ACTGCCCACTGAGGAAGTGAGGAGCCAGGCTTCAGACGACAGCTCCCTGGGCAAGAGCACCAACATCCTG ATGATCCCTAACCCCCATCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATG TCCTGGAGAACATGATAGAACCACCACAGCGGGACTCTAGTGCACCTGGAAGGTTCCACGTGGGCAGTGC AGAGTCCATGCTACATGTTCGACCTGGAGGCTACACCCCTCAGAGAGCACTGATTAACCCCTTCGCCCCC TCAAGAATGCCCATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCATACTTTTCCTGTAGGACCATCTG GAGAGGCCATCCAGATCCATCATCAAACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGCGGGA CCCCACCCACTCCTCTGCAGAACTGTTGGAGCTGGCATATCATGAAGCCGCAGGAAGGCACAGCACTTCC CGACAGCCTGGTGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCA ACAGCGGCACAGGTATGAATCCTAGGAACCAGAATAAGGACTCTCTAGAGGATGGTGTTTCTACCTCTCC AGACCCAATGCCAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTACTACTCCTGCATGCCTT CCCCTCACCACTGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACAGAGGGCTGCTATGATC TCCTCCCAGAAGCAGACATGGACAGGAGGGACGAGGAGGGCGTTCAAATGACAGCCCAGCAAGTGTTTGA AGAGTTCATCTGCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAAGACCCAGAAGCCCAGC ACTACAGTCCCACCCCCGCTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTGTCCCGAAACCGCCCTG AAGAGGAGGGCCAGTATTGGCTGAGTATGGGCAGAACCTTCCATAAGGTGACACTTAAGGACAAGATGAT CACAGTGACTCGTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTACACCTACAGCCTCTGC CCTTCCCACTCTGACTCAGAGTTTGTCTCCTGTTGGGTGGAATTCTGCCATGAACGGCTAGAGGAATACA AGTGGAATTACTTAGATCAGTACATTTGTTCTGCCGGCTCCGAAGATTTCAGCTTAATCGAGTCTCTGAA GTTCTGGAGAACCCGATTTCTACTACTGCCAGCCTGTGTCACTGCCACCAAGCGCATCACAGAAGGGGAA GTGCACTGTGACATCTACGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAGCTTCTAGATGGCTTTA TTCGCTTTGTAGAGGGCTTGAACCGCATCCGCAGGCGCCACCGGTCAGACCGCATGATTCGGAAAGGGAC TGCCATGAAAGGCTTGCAGATGACCGGGCCCATCTCCGCACACTCTCTCGAGTCAACGGGCCCCCCAGTC GGGAAGAAAGGAACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAGGCTAGTCAGAAGAGCCTAGGAGAAC AGCAGACAAGTGTGCATGGGAAAAGCTCTACTCAGCCAGCTGAGAACAGCAGTGTTGCCATGACTCCCAC CTATGTGGACAGCCCACGCAAGGCATCTGTGGACCAAACAGCTCCTCTGGTGTTGGACAGCACCAGTTTA GGGGTCAGCACGTGCCAGTCCGTGGATAGAGGCAATAACCAGACCTTTGGGAACTCCCAGAACATAGAAC AGGCCTTTCCCTCTACAGGCTCTGGAGACGGCAGCTCTCAGCAGCACATAGCCAGCTCTCTGACATCGTC CTCTACCCTGGTGGAGATCCTGGAAGCCATGAAGCATCCCTCGACAGGAGTCCAGCTGCTGTCTGAGCAG AAGGGCCTTTCGCCGTGCTGCTTCATCAGTGCGGAGGTCGTACACTGGCTGATGAACAATGTGGAGGGGG TCCAGACGCAGGCGATGGGCATCGACATCATGCAGAAAATGCTGGAAGAGCAGCTCATAGCGCATGCATC TGGAGAAGCATGGCGAACCTTCGTCTACGGCTTCTATTTCTACAAGATAGTCACGGACAGAGAGCCCGAA CGAGTGGCTATGCAGCAGCCCTCTGCCCCATGGCACACAGCAGGAGTGGATGACTTTGCCAGCTTCCAAC GCAAGTGGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTTGTGCACTCCGAGATTCCTGCCTTCCTCCTGCC CTGGCTACCTAGCCGGCCAGCCTCTTACGCAAGCAGGCACAGCTCCTTTAGCCGAAGTTTTGGAGGACGG AGCCAGGCAGCTGCACTGTTAGCTGCCACTGTCCCAGAGCAGAGGACTGTGACCCTGGATGTCGATGTGA ACAACCGAACGGACCGGCTGGAGTGGTGCAGCTGTTACTACCACGGAAACTTCTCTCTCAACGCAGCCTT TGAAATCAAGCTACACTGGATGGCAGTGACTGCCACAGTGCTGTTTGAGATGGTCCAAGGTTGGCATCGA AAAGCTACATCCTGCGGCTTCTTGTTAGTCCCGGTTTTGGAGGGTCCTTTTGCACTGCCCAGTTACCTGT ACGGTGACCCCTTGAGGGCCCAGCTCTTCATCCCGCTCAACCTCGGCTGCTTGCTCAAGGAGGGCAGCGA GCACCTGTTCGATAGCTTTGAACCAGAGACATATTGGGATCGAATGCACCTTTTCCAGGAAGCCATTGCA CACAGGTTTGGATTTGTGCAAGATAAATATTCTGCCTCCGCGTTTAACTTCCCTGCTGAGAATAAACCAC AGTATATCCACGTCACAGGAACAGTGTTTCTTCAGCTGCCGTACTCCAAACGCAAGTTTTCTGGGCAGCA GCGGCGGCGACGGAACTCTACCAGCTCCACCAACCAAAACATGTTCTGTGAGGAGCGGGTTGGCTACAAC TGGGCCTATAACACCATGCTGACCAAGACATGGCGCTCTAGTGCCACGGGGGATGAGAAGTTTGCCGATC GGCTGCTGAAGGACTTCACGGATTTCTGTATCAACCGTGACAACCGCCTGGTCACATTCTGGACAAACTG CCTGGAGAAGATGCACGCCAGCGCTCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107229
Insert Size	4371 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107229.1, NP_001100699.1
RefSeq Size	8076 bp
RefSeq ORF	4371 bp
Locus ID	305464

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