Egfr (NM_031507) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ErbB-1; ERBB1; Errp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206244 representing NM_031507 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACCCTCAGGGACTGCGAGAACCAAGCTACTGCTGCTGCTGGCTGCGCTCTGCGCCGCAGGTGGGG CGCTGGAGGAAAAGAAAGTTTGCCAAGGCACAAGTAACAGGCTCACCCAACTAGGCACCTTTGAAGACCA CTTTCTGAGCCTCCAGAGGATGTTCAACAACTGTGAAGTGGTCCTTGGAAACTTGGAAATCACCTATGTG CAAAGGAATTATGACCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCTGGCTATGTTCTCATTGCCCTGA ACACCGTGGAGAGAATCCCTTTGGAGAACCTGCAGATCATCAGGGGAAATGCTCTCTACGAAAACACCTA CGCCTTAGCCGTCCTGTCCAACTATGGAACCAACAAAACTGGGCTTAGGGAACTGCCCATGCGGAACTTA CAGGAAATTCTGATCGGTGCTGTGCGATTTAGCAACAACCCCATCCTCTGCAATATGGAGACCATCCAGT GGAGGGACATCGTCCAAGATGTCTTTCTGAGCAACATGTCAATGGACGTACAGCGCCACCTGACGGGCTG CCCGAAATGTGATCCGAGCTGTCCCAATGGAAGCTGCTGGGGAAGAGGAGAGGAGAACTGCCAGAAATTG ACCAAAATCATCTGCGCCCAGCAATGTTCCCGGCGTTGTCGTGGCAGGTCCCCTAGCGACTGCTGCCACA ACCAGTGTGCCGCAGGGTGTACAGGGCCCAGAGAGAGTGACTGTCTGGTCTGCCACAGGTTCCGAGATGA AGCCACGTGCAAAGACACCTGCCCACCACTCATGCTGTACAACCCCACCACGTACCAGATGGATGTCAAC CCTGAGGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAATGCCCCAGAAACTACGTGGTGACAGATC ACGGCTCGTGTGTCCGGGCCTGTGGGCCAGACTACTATGAAGTAGAAGAAGATGGAGTCAGCAAGTGTAA AAAATGTGACGGGCCCTGCCGCAAAGTTTGCAATGGCATAGGCATTGGTGAATTTAAAGACACACTCTCC ATAAATGCTACAAACATCAAACACTTCAAGTACTGCACTGCCATCAGTGGGGACCTCCACATCCTGCCAG TGGCCTTTAAGGGGGATTCTTTCACCCGCACTCCTCCTCTAGACCCACGGGAACTAGAAATTCTCAAAAC TGTGAAGGAAATAACAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACTGGACTGACCTCCATGCTTTT GAGAACCTAGAAATAATTCGTGGCAGAACAAAGCAACATGGTCAGTTTTCTCTGGCGGTTGTCGGCCTGA ACATAACATCGCTGGGGTTGCGTTCCCTCAAGGAGATCAGTGATGGGGATGTGATTATTTCTGGGAACCG AAATTTGTGCTACGCAAACACTATAAACTGGAAAAAACTCTTCGGGACGCCCAATCAAAAGACCAAAATC ATGAACAACAGAGCTGAAAAGGACTGCAAGGCCACGAACCACGTCTGTAATCCTTTATGCTCCTCGGAAG GCTGCTGGGGCCCTGAGCCCACGGACTGTGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGA CAAGTGCAACATCCTGGAGGGGGAACCGAGGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCA GAATGTCTGCCCCAGACCATGAACATCACCTGTACAGGCCGGGGGCCAGACAACTGCATCAAGTGTGCCC ACTATGTTGATGGTCCCCACTGTGTCAAGACCTGCCCTTCGGGCATCATGGGGGAGAACAACACCCTGGT CTGGAAGTTTGCAGATGCCAATAACGTCTGCCACCTCTGCCATGCAAACTGTACCTATGGATGTGCTGGG CCAGGCCTTAAAGGATGTCAACAACCAGAAGGGCCAAAGATCCCATCCATCGCCACTGGGATTGTGGGTG GCCTCCTCTTCATAGTAGTGGTGGCCCTTGGGATCGGCCTCTTCATGCGTCGACGTCAGCTTGTCCGAAA ACGTACACTACGCCGCCTGCTTCAAGAGAGAGAGCTCGTGGAACCTCTCACACCCAGCGGAGAAGCTCCG AACCAAGCCCACTTGAGGATATTAAAGGAAACAGAATTCAAAAAGATCAAAGTTCTGGGTTCAGGAGCAT TTGGCACAGTGTATAAGGGTCTCTGGATCCCAGAAGGCGAGAAAGTGAAAATCCCTGTGGCCATCAAGGA GTTAAGAGAAGCCACATCTCCCAAAGCCAACAAGGAAATCCTTGATGAAGCCTACGTGATGGCCAGTGTG GACAACCCTCATGTATGCCGCCTCCTGGGCATCTGTCTGACCTCCACTGTCCAGCTCATTACACAACTCA TGCCCTATGGTTGCCTCCTGGACTATGTCCGAGAACATAAGGACAACATTGGCTCCCAGTACCTACTCAA CTGGTGTGTGCAGATTGCAAAGGGCATGAACTACCTGGAAGACCGGCGTTTGGTACACCGTGACTTGGCA GCCAGGAATGTACTGGTAAAGACACCACAGCATGTCAAGATCACAGATTTTGGACTGGCCAAACTGCTTG GTGCTGAGGAGAAAGAATACCATGCAGAGGGGGGCAAAGTGCCTATCAAGTGGATGGCTTTGGAATCAAT TTTACACCGAATTTATACACACCAAAGCGACGTCTGGAGCTATGGAGTCACCGTGTGGGAACTGATGACC TTTGGGTCCAAGCCTTATGATGGGATCCCTGCAAGTGAGATCTCATCCATCCTAGAGAAAGGAGAGCGCC TTCCACAGCCACCTATCTGCACCATCGACGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGCTGA TAGCCGCCCAAAGTTCCGAGAGTTGATTCTCGAATTCTCCAAAATGGCCAGAGACCCACAGCGCTACCTT GTTATCCAGGGGGATGAAAGGATGCATTTGCCGAGCCCTACAGACTCCAACTTTTACCGAGCCCTGATGG AGGAGGAGGACATGGAAGACGTAGTTGATGCTGATGAATACCTCATCCCACAGCAAGGCTTCTTCAACAG CCCATCCACGTCACGGACTCCACTCTTGAGCTCTCTGAGTGCAAATAGCAACAGTTCCACTGTGGCTTGC ATTAATAGAAATGGGAGCTGCCGTGTCAAAGAAGACGCCTTCTTGCAACGGTATAGCTCCGATCCCACCA GCGTCCTGACAGAGGACAACATAGATGACACATTCCTTCCCGTGCCTGAATATATAAACCAATCTGTTCC CAAGAGGCCGGCTGGCTCTGTGCAGAACCCAGTCTATCACAATCAGCCCCTGCATCCAGCTCCTGGAAGA GACCTGCATTATCAAAATCCCCATAGCAATGCGGTGAGCAACCCTGAGTATCTCAACACTGCCCAGCCGA CCTGCCTCAGTAGTGGGTTTGACAGCTCTGCCCTCTGGATCCAGAAAGGCAGCCACCAAATGAGCCTGGA CAACCCTGACTACCAGCAGGACTTCTTTCCCAAAGAAGCCAAGCCGAATGGCATCTTTAAGGGCCCCACA GCTGAAAATGCAGAGTACCTGCGGGTGGCACCGCCAAGCAGTGAGTTTAGTGGAGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031507
Insert Size	3630 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031507.1, NP_113695.1
RefSeq Size	4161 bp
RefSeq ORF	3630 bp
Locus ID	24329
Gene Summary	promotes cell proliferation and differentiation; mediates GPCR regulated induction of protein synthesis [RGD, Feb 2006]

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