Egfr (NM_031507) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ErbB-1; ERBB1; Errp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206244 representing NM_031507 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACCCTCAGGGACTGCGAGAACCAAGCTACTGCTGCTGCTGGCTGCGCTCTGCGCCGCAGGTGGGG CGCTGGAGGAAAAGAAAGTTTGCCAAGGCACAAGTAACAGGCTCACCCAACTAGGCACCTTTGAAGACCA CTTTCTGAGCCTCCAGAGGATGTTCAACAACTGTGAAGTGGTCCTTGGAAACTTGGAAATCACCTATGTG CAAAGGAATTATGACCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCTGGCTATGTTCTCATTGCCCTGA ACACCGTGGAGAGAATCCCTTTGGAGAACCTGCAGATCATCAGGGGAAATGCTCTCTACGAAAACACCTA CGCCTTAGCCGTCCTGTCCAACTATGGAACCAACAAAACTGGGCTTAGGGAACTGCCCATGCGGAACTTA CAGGAAATTCTGATCGGTGCTGTGCGATTTAGCAACAACCCCATCCTCTGCAATATGGAGACCATCCAGT GGAGGGACATCGTCCAAGATGTCTTTCTGAGCAACATGTCAATGGACGTACAGCGCCACCTGACGGGCTG CCCGAAATGTGATCCGAGCTGTCCCAATGGAAGCTGCTGGGGAAGAGGAGAGGAGAACTGCCAGAAATTG ACCAAAATCATCTGCGCCCAGCAATGTTCCCGGCGTTGTCGTGGCAGGTCCCCTAGCGACTGCTGCCACA ACCAGTGTGCCGCAGGGTGTACAGGGCCCAGAGAGAGTGACTGTCTGGTCTGCCACAGGTTCCGAGATGA AGCCACGTGCAAAGACACCTGCCCACCACTCATGCTGTACAACCCCACCACGTACCAGATGGATGTCAAC CCTGAGGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAATGCCCCAGAAACTACGTGGTGACAGATC ACGGCTCGTGTGTCCGGGCCTGTGGGCCAGACTACTATGAAGTAGAAGAAGATGGAGTCAGCAAGTGTAA AAAATGTGACGGGCCCTGCCGCAAAGTTTGCAATGGCATAGGCATTGGTGAATTTAAAGACACACTCTCC ATAAATGCTACAAACATCAAACACTTCAAGTACTGCACTGCCATCAGTGGGGACCTCCACATCCTGCCAG TGGCCTTTAAGGGGGATTCTTTCACCCGCACTCCTCCTCTAGACCCACGGGAACTAGAAATTCTCAAAAC TGTGAAGGAAATAACAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACTGGACTGACCTCCATGCTTTT GAGAACCTAGAAATAATTCGTGGCAGAACAAAGCAACATGGTCAGTTTTCTCTGGCGGTTGTCGGCCTGA ACATAACATCGCTGGGGTTGCGTTCCCTCAAGGAGATCAGTGATGGGGATGTGATTATTTCTGGGAACCG AAATTTGTGCTACGCAAACACTATAAACTGGAAAAAACTCTTCGGGACGCCCAATCAAAAGACCAAAATC ATGAACAACAGAGCTGAAAAGGACTGCAAGGCCACGAACCACGTCTGTAATCCTTTATGCTCCTCGGAAG GCTGCTGGGGCCCTGAGCCCACGGACTGTGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGA CAAGTGCAACATCCTGGAGGGGGAACCGAGGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCA GAATGTCTGCCCCAGACCATGAACATCACCTGTACAGGCCGGGGGCCAGACAACTGCATCAAGTGTGCCC ACTATGTTGATGGTCCCCACTGTGTCAAGACCTGCCCTTCGGGCATCATGGGGGAGAACAACACCCTGGT CTGGAAGTTTGCAGATGCCAATAACGTCTGCCACCTCTGCCATGCAAACTGTACCTATGGATGTGCTGGG CCAGGCCTTAAAGGATGTCAACAACCAGAAGGGCCAAAGATCCCATCCATCGCCACTGGGATTGTGGGTG GCCTCCTCTTCATAGTAGTGGTGGCCCTTGGGATCGGCCTCTTCATGCGTCGACGTCAGCTTGTCCGAAA ACGTACACTACGCCGCCTGCTTCAAGAGAGAGAGCTCGTGGAACCTCTCACACCCAGCGGAGAAGCTCCG AACCAAGCCCACTTGAGGATATTAAAGGAAACAGAATTCAAAAAGATCAAAGTTCTGGGTTCAGGAGCAT TTGGCACAGTGTATAAGGGTCTCTGGATCCCAGAAGGCGAGAAAGTGAAAATCCCTGTGGCCATCAAGGA GTTAAGAGAAGCCACATCTCCCAAAGCCAACAAGGAAATCCTTGATGAAGCCTACGTGATGGCCAGTGTG GACAACCCTCATGTATGCCGCCTCCTGGGCATCTGTCTGACCTCCACTGTCCAGCTCATTACACAACTCA TGCCCTATGGTTGCCTCCTGGACTATGTCCGAGAACATAAGGACAACATTGGCTCCCAGTACCTACTCAA CTGGTGTGTGCAGATTGCAAAGGGCATGAACTACCTGGAAGACCGGCGTTTGGTACACCGTGACTTGGCA GCCAGGAATGTACTGGTAAAGACACCACAGCATGTCAAGATCACAGATTTTGGACTGGCCAAACTGCTTG GTGCTGAGGAGAAAGAATACCATGCAGAGGGGGGCAAAGTGCCTATCAAGTGGATGGCTTTGGAATCAAT TTTACACCGAATTTATACACACCAAAGCGACGTCTGGAGCTATGGAGTCACCGTGTGGGAACTGATGACC TTTGGGTCCAAGCCTTATGATGGGATCCCTGCAAGTGAGATCTCATCCATCCTAGAGAAAGGAGAGCGCC TTCCACAGCCACCTATCTGCACCATCGACGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGCTGA TAGCCGCCCAAAGTTCCGAGAGTTGATTCTCGAATTCTCCAAAATGGCCAGAGACCCACAGCGCTACCTT GTTATCCAGGGGGATGAAAGGATGCATTTGCCGAGCCCTACAGACTCCAACTTTTACCGAGCCCTGATGG AGGAGGAGGACATGGAAGACGTAGTTGATGCTGATGAATACCTCATCCCACAGCAAGGCTTCTTCAACAG CCCATCCACGTCACGGACTCCACTCTTGAGCTCTCTGAGTGCAAATAGCAACAGTTCCACTGTGGCTTGC ATTAATAGAAATGGGAGCTGCCGTGTCAAAGAAGACGCCTTCTTGCAACGGTATAGCTCCGATCCCACCA GCGTCCTGACAGAGGACAACATAGATGACACATTCCTTCCCGTGCCTGAATATATAAACCAATCTGTTCC CAAGAGGCCGGCTGGCTCTGTGCAGAACCCAGTCTATCACAATCAGCCCCTGCATCCAGCTCCTGGAAGA GACCTGCATTATCAAAATCCCCATAGCAATGCGGTGAGCAACCCTGAGTATCTCAACACTGCCCAGCCGA CCTGCCTCAGTAGTGGGTTTGACAGCTCTGCCCTCTGGATCCAGAAAGGCAGCCACCAAATGAGCCTGGA CAACCCTGACTACCAGCAGGACTTCTTTCCCAAAGAAGCCAAGCCGAATGGCATCTTTAAGGGCCCCACA GCTGAAAATGCAGAGTACCTGCGGGTGGCACCGCCAAGCAGTGAGTTTAGTGGAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206244 representing NM_031507 Red=Cloning site Green=Tags(s) MRPSGTARTKLLLLLAALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYV QRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAVLSNYGTNKTGLRELPMRNL QEILIGAVRFSNNPILCNMETIQWRDIVQDVFLSNMSMDVQRHLTGCPKCDPSCPNGSCWGRGEENCQKL TKIICAQQCSRRCRGRSPSDCCHNQCAAGCTGPRESDCLVCHRFRDEATCKDTCPPLMLYNPTTYQMDVN PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGVSKCKKCDGPCRKVCNGIGIGEFKDTLS INATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPENWTDLHAF ENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKI MNNRAEKDCKATNHVCNPLCSSEGCWGPEPTDCVSCQNVSRGRECVDKCNILEGEPREFVENSECIQCHP ECLPQTMNITCTGRGPDNCIKCAHYVDGPHCVKTCPSGIMGENNTLVWKFADANNVCHLCHANCTYGCAG PGLKGCQQPEGPKIPSIATGIVGGLLFIVVVALGIGLFMRRRQLVRKRTLRRLLQERELVEPLTPSGEAP NQAHLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASV DNPHVCRLLGICLTSTVQLITQLMPYGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLA ARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMT FGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELILEFSKMARDPQRYL VIQGDERMHLPSPTDSNFYRALMEEEDMEDVVDADEYLIPQQGFFNSPSTSRTPLLSSLSANSNSSTVAC INRNGSCRVKEDAFLQRYSSDPTSVLTEDNIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLHPAPGR DLHYQNPHSNAVSNPEYLNTAQPTCLSSGFDSSALWIQKGSHQMSLDNPDYQQDFFPKEAKPNGIFKGPT AENAEYLRVAPPSSEFSGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031507
ORF Size	3627 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031507.1, NP_113695.1
RefSeq Size	4161 bp
RefSeq ORF	3630 bp
Locus ID	24329
MW	134.9 kDa
Gene Summary	promotes cell proliferation and differentiation; mediates GPCR regulated induction of protein synthesis [RGD, Feb 2006]

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