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Elovl2 (NM_001109118) Rat Untagged Clone
CAT＃: RN206070
Elovl2 (untagged ORF) - Rat elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 2 (Elovl2), (10 ug)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN206070 representing NM_001109118
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGACCACGGGATTCTCGAGTTCGAGGGTGGTTCCTGCTGGACTCTTACCTCCCCACCTTCACCC TCACCATCGTGTATCTGCTCTCAATATGGCTGGGTAACAAGTACATGAAGAACAGGCCTGCTCTTTCTCT CAGGGGAATCCTCACCTTGTATAACCTCGGAATCACACTTCTTTCTGCATATATGCTGGTGGAGCTCGTT CTCTCCAGCTGGGAAGGAGGCTACAACTTGCAGTGTCAGAATCTGGACAGCGCAGGAGAAGGCGATATCC GGGTAGCCAAGGTCTTGTGGTGGTACTACTTCTCCAAACTGGTGGAGTTCCTGGACACGATCTTCTTTGT TCTTCGGAAAAAGACCAGTCAGATCACCTTCCTTCATGTCTATCACCATGCGTCCATGTTTAACATCTGG TGGTGTGTTTTGAACTGGATACCTTGTGGTCAAAGCTTCTTCGGACCAACCCTGAACAGCTTTATCCACA TCCTCATGTACTCCTACTACGGGCTGTCTGTGTTCCCGTCCATGCACAGGTACCTTTGGTGGAAGAAATA CCTCACGCAGGCCCAGCTGGTACAGTTTGTACTGACCATCACGCACACGCTGAGTGCCGTGGTGAAGCCC TGTGGCTTCCCCTTTGGCTGTCTCATCTTCCAGTCTTCCTACATGATGACACTGGTTATCCTGTTCTTAA ACTTCTATATTCAGACATACCGAAAAAAGCCAATGAAGAAGGAGATGCCAGAAGGAGCCGCAGGGAAAGA AGTGAAGAATGGTTTCCCCAAAGCCCACTCCATCGCAGCTAATGGCGTGACGGACAAGAAGGTGCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001109118 |
| Insert Size | 840 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001109118.1, NP_001102588.1 |
| RefSeq Size | 3731 bp |
| RefSeq ORF | 840 bp |
| Locus ID | 498728 |
| UniProt ID | D4A612 |
| Gene Summary | Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of polyunsaturated very long chain fatty acid (C20- and C22-PUFA), acting specifically toward polyunsaturated acyl-CoA with the higher activity toward C20:4(n-6) acyl-CoA. May participate in the production of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR206070 | Elovl2 (Myc-DDK-tagged ORF) - Rat elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 2 (Elovl2), (10 ug) |
CNY 3,990.00 |
|
| RR206070L3 | Lenti ORF clone of Elovl2 (Myc-DDK-tagged ORF) - Rat elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 2 (Elovl2), (10 ug) |
CNY 6,080.00 |
|
| RR206070L4 | Lenti ORF clone of Elovl2 (mGFP-tagged ORF) - Rat elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 2 (Elovl2), (10 ug) |
CNY 6,650.00 |
