Auh (NM_001108407) Rat Untagged Clone

CAT#: RN205989

Auh (untagged ORF) - Rat AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), nuclear gene encoding mitochondrial protein, (10 ug)



  "NM_001108407" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN205989 representing NM_001108407
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCATTGTGGTGCTTGGAATTAACAGAGCTTATGGGAAAAATTCACTCAGTAAAAATCTTCTCAAAA
TGTTATCAAAAGCGGTGGACGCATTAAAATCGGATAAGAAAGTTCGGACCATTATCATCAGAAGCGAAGT
CCCTGGGATATTCTGTGCTGGCGCTGACCTTAAGGAAAGAGCCAAGATGCATTCCAGTGAAGTCGGTCCC
TTTGTCTCCAAGATCCGAGCAGTGATCAATGACATCGCTAACCTTCCTGTGCCCACCATCGCTGCCATAG
ATGGCCTGGCCCTAGGAGGGGGCCTGGAGCTGGCTTTAGCGTGTGACATTCGAGTAGCAGCTTCTTCTGC
AAAAATGGGCCTGGTGGAAACGAAGTTGGCGATTATCCCTGGTGGAGGAGGGACACAGAGATTACCACGT
GCCATCGGGATGGCCCTGGCAAAGGAGCTCATCTTCTCTGCACGCGTTCTTGACGGTCAGGAGGCCAAAG
CCGTGGGCTTGATCAGCCACGTGTTAGAACAGAACCAGGAAGGGGATGCTGCCTACAGGAAGGCGCTGGA
CCTGGCAAGAGAGTTTCTACCTCAGGGGCCTGTTGCCATGAGAGTGGCCAAACTAGCAATTAACCAAGGC
ATGGAGGTTGACTTAGTGACAGGGTTAGCCATAGAAGAAGCCTGCTATGCTCAGACCATCTCAACGAAGG
ACAGACTGGAAGGTCTTCTTGCTTTCAAGGAGAAAAGACCCCCTCGCTACAAGGGAGAGTAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001108407
Insert Size 762 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001108407.1, NP_001101877.1
RefSeq Size 1246 bp
RefSeq ORF 762 bp
Locus ID 361215
Gene Summary Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (By similarity). Also has itaconyl-CoA hydratase activity by converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed:13783048). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:13783048). Has very low enoyl-CoA hydratase activity (By similarity). Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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