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Auh (NM_001108407) Rat Untagged Clone
CAT＃: RN205989
Auh (untagged ORF) - Rat AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), nuclear gene encoding mitochondrial protein, (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205989 representing NM_001108407
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATTGTGGTGCTTGGAATTAACAGAGCTTATGGGAAAAATTCACTCAGTAAAAATCTTCTCAAAA TGTTATCAAAAGCGGTGGACGCATTAAAATCGGATAAGAAAGTTCGGACCATTATCATCAGAAGCGAAGT CCCTGGGATATTCTGTGCTGGCGCTGACCTTAAGGAAAGAGCCAAGATGCATTCCAGTGAAGTCGGTCCC TTTGTCTCCAAGATCCGAGCAGTGATCAATGACATCGCTAACCTTCCTGTGCCCACCATCGCTGCCATAG ATGGCCTGGCCCTAGGAGGGGGCCTGGAGCTGGCTTTAGCGTGTGACATTCGAGTAGCAGCTTCTTCTGC AAAAATGGGCCTGGTGGAAACGAAGTTGGCGATTATCCCTGGTGGAGGAGGGACACAGAGATTACCACGT GCCATCGGGATGGCCCTGGCAAAGGAGCTCATCTTCTCTGCACGCGTTCTTGACGGTCAGGAGGCCAAAG CCGTGGGCTTGATCAGCCACGTGTTAGAACAGAACCAGGAAGGGGATGCTGCCTACAGGAAGGCGCTGGA CCTGGCAAGAGAGTTTCTACCTCAGGGGCCTGTTGCCATGAGAGTGGCCAAACTAGCAATTAACCAAGGC ATGGAGGTTGACTTAGTGACAGGGTTAGCCATAGAAGAAGCCTGCTATGCTCAGACCATCTCAACGAAGG ACAGACTGGAAGGTCTTCTTGCTTTCAAGGAGAAAAGACCCCCTCGCTACAAGGGAGAGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001108407 |
| Insert Size | 762 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001108407.1, NP_001101877.1 |
| RefSeq Size | 1246 bp |
| RefSeq ORF | 762 bp |
| Locus ID | 361215 |
| Gene Summary | Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (By similarity). Also has itaconyl-CoA hydratase activity by converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed:13783048). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:13783048). Has very low enoyl-CoA hydratase activity (By similarity). Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205989 | Auh (Myc-DDK-tagged ORF) - Rat AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 3,990.00 |
|
| RR205989L3 | Lenti ORF clone of Auh (Myc-DDK-tagged ORF) - Rat AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,080.00 |
|
| RR205989L4 | Lenti ORF clone of Auh (mGFP-tagged ORF) - Rat AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,650.00 |
