Ncapd3 (NM_001034000) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205953 representing NM_001034000 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGAAGGATCTAGGAAGAGACCTGCAGCCTTGGTGTCCGCTGGATCTCAGCCTTGAATGGGTGA ACACAGTGTGGGACCTGGACTTCACGGAGACCGAACCTTTGGATCCCAGCGTGGTAGCAGAGATCCTAGA GACTGGAAGAGATGCGTTCACAAAGCTCTATGGGAGCCTCTTCCCCTTTGCAACCGACGAAAGTGGCTCT TTAGAGAGCGTCTGGTCCTTCTTCACTGAGAACAGCATTTCCAGCAACACTCTGGTGGCGTTGTTCTGTC ACTTTGTCCAGGAAGCCCATAAAAAAAGTGCCAGTGTGCAATATCGAGAATATGGCCTTCGTGCTGCGGG GCTCTATTTCCTGCTGCTAGAAATACCAGGCAGCGTAGTCAATCAAGTATTCCACCCAGTGATGTTCGAC AAGTGTATTCAGATCCTGAAGAAGAGCTGGCCTCAAGAATCCAACTTGACTCAGAAGAGAAAAAAGGAAC ACTCCAAGAGTTCTAAGGCTAATTACAGGAGGAGCAGAAAAAGAGGAAAACCACCTAGGAAAGAGGATTA TCAGGTGGATGAACTTTCAAGAGAGGAAGAGGAGGAGGAGGAAGAGGAGATTTATTTTTCTGTCCGGGAC CTTTCTCAAATACGAGATGCCATATTTAATCTTCTAAAGAATTTTTTAAGGCTTCTGCCCAAGTTTTCTC TTAAAGAAAAGCCGCAGTCTATTCAAACATGTATAGAGGTGTTTGTTGCATTAACTAGTTTTGAACCAAT TCCTCATAAATTTCTTATTTCACAAGCCAGGAACCTCAGTGAAGTAAAACACATTTCAGAGCTGGCCTAC TATGGATTGTACTTGCTGTGCTCCCCAGTTCATGGAGAAGGAAATAAGGTTATCGGTTCTATTTTTCATC AAATGCTCAATGTGATACTGATGTTAGAGGTAGGAGAAGGGTCCCGCTGTGCTCCTCTTGCCATAACCTC ACACGTCATCAATTGTAGAAACCAAGCAGTCCAGTTTGTCAGCTCACTTGTGGATGAACTACAAGAAAGT GTGTTCCCCATCCTTGGTACTTTACTGCAGCACATCTGTGCCAAGGTGGTGGATAAAGCAGAATATCGGA CCTACGCAGCACAGTCCCTGGTCCAGCTGCTTAGCAAACTTCCCTCCAAGGAATATGCTACATTCATTGC CTGGCTTTACAAATATTCACGAAGTTCCAAGATCCCACACCGGGTCTTCACTCTTGATGTTGCTTTAGCT CTGTTAGAGCTGCCCGAAAGACTGCTGGATGACACTGTGCTACTGGAGCATCAGAAGTTTCTAAAGCATA AATTCTTTGTGCAAGAAATTATATTTGATCGTTGCTTAGATAAGGCACCTACTGTCCGCAGCAAGGCGCT GTCCAGCTTTGCACAGTGTTTGGAGTTGTCTACTAGCAACACATCTGAGAACACACTGGAAATCTTCATT AACAGTACTGTGACAGGAATACACAGTCACTTTGGCACCTCACTGAGACCCTCGACAGTGTTAACCAGCC AAAGTTGCCGCTCAGTACAAAGCAGCACCCATGACAGTGATGAACAGACTCCGCCTGGAGAGAGACGCTT CATGACAATGCTGAGAAAAAGAACCAAAGATGAGAAGATCAACGTTAGGAAGTCTGCACTCCAGGTGTTA ATGAGTATTTTGAAACACTGTGACATCTTGAGCATGGAGGAGGACCTGTTGATCCTGCAGGACCACTGTC GAGACCCTGCCATATCTGTGCGGAAGCAAGCCCTACAGTCTCTTACAGAACTTGTCATGGCCAAGCCTAC TTGTGTCCCAGTGCAGAAGGCCTGGCTGATGGGTGTCATCCCTGTGGTGATGGACTATGAGAGCACAGTG CAAGAGAAGGCCTTGGAGTGCCTGGACCAGCTCCTGCTGCAGAACATTAAGCACCATAGGAAGTTTCACA GCGAGGACAGGAGTCAGGTGCTCGCCTGGGCCCTACTTGCTCTCCTCACCGTAGAAAGCCAAGATCTGAG CCGCTATTTAAATAAGGCTTTTCATATCTGGTCCAAGAAAGATAAATTCTCATCCACTTTTATAAACAGT GTGATATCCCACACTGACACAGAACGTTCTGCGCCAGCCTGGATGCTGCTCTCCAAGATCACTTGCTCGT CACCCAAGCTGGACTATACCAAAATAATGGAGTCGTGGGAGAAACTCAGCAGAGAACAATCTCCCAACTC AAACACCTTAGGCTACATGCTGTGTGTCATTGGGCATATTGCAAAGCATCTTCCAAAGAGCACACAGGAC AAGATAACTGGTGTGATCAAGGCCAAGCTGAATGGATTTCAGTGGTCTCTAGAGCTGATCAGTTTGTCCG TTGACACATTACAAGGGCTTTGCAGAGCATCTGCAAAGACACTGCGGGAAGAGCAGGAACTGCTGAAGCG GGTGTGTGGGGATGTCCTCTCCACCTGTGAGCAACACCTCTCTAACATCCTTCTGAAGGAAGACGGGACA GGGAACATGGATGAAGACCTGGTGGTGAAATGCATTTTTACCCTAGGAGATATAGCCCAGTTATGTCCAG CCCTAGTAGAGAAGCGAGTCTTCCTTCTAATCCAGTCTATTCTGGCTTCTTCTGCTCATGCGGATCACTT GCCATCATCTCAAGGGACCACAGACCTCGCCAGTCAGCTGCCTTTCCAGGCCAGGAGCTCTGCCCTGCCG TCTGTGATTAGAGCACATGCCATCATCACTCTGGGTAAACTCTGCTTACAGCATGAAGACCTTGCAAAGA AAAGCATCCCAGCCCTTGTGCGAGAACTTGAAGTCTGTGAGGATGTGGCTGTCCGCAACAATGTCATCAT TGTGATGTGTGATCTTTGTATCCGGTACACTGTCATGGTGGACAATTATATTCCCAATATTTCCATTTGC CTGAAGGATTCAGACCCGTTCATCCGAAAGCAGACACTGATCTTGCTTACCAACCTCTTACAGGAGGAAT ATGTGAAGTGGAAGGGCTCTCTGTTCTTCCGATTTGTCAGCACTCTGGTAGACTCACACCCAGACATTGC CAGCCTTGGGGAGTTCTGCCTGGCTCACTTGTTGCTGAAGAGGAACCCTACCATGTTTTTCCAGCACTTC ATTGAGTGCATATTCCACTTCAACAGCTATGAGAAGCATGGGCAGTATAACAAGTTTTCCCAGTCGGAGA GGGGGAAGCCACTGTTTTTGCTAAAGGGAAAGACAAACAAGGAGAAGCGAATGAGAATCTACAAGTTTCT TCTTGAGCACTTCACAGATGAGCAGCGATTCAATGTCACTTCCAAGATCTGTCTTAACATTTTGGCGTGC TTCACGGATGGCGTCCTGCCCGTGGACATGGAAGCCAGCGAGCTGCTCTCCGATACCTTTGACATCCTCA GCTCTAAGGAGATCAAACTATTGGCAATGAGAGCGCAGGCATCCAAGGAGCTCCTGGAGGAAGATGACGT GGCTCTGGCGAATGTGGTCATGCAGGAGGCCCAGATGAAGATTATCTCGCAGGTTCAAAAGAGGAATTTC ATAGAGAATATTATTCCAATCATCATCTCCCTGAAGACTGTGCTGGAGAAAAATAAGATCCCTGCTTTAA GGGAACTCATGAACTACCTCAGGGAAGTAATGCAGGATTACCGCGATGAGATCAAAGACTTCTTTGCAGT GGACAGACAGCTGGCGTCTGAGCTTGAGTATGACATGAAGAAGTACACTGAACAACTGGCCCAGGAGCAA GCTCTGACGGAGCATGCTAATGCCACCAACGTGCCTGCAGACGGGGACAGGGTCCCCTCCCAGCAGGTTG CTCCAGACTTACAAGCTGTACCTGGCGCTGCTGCCGCCGCTGGTGCTGGCCAGGCCGATGCTAACATGCC TCTGCCTCTGGCCCAGAGCCAGCCTTCAGCACCTGGGTCCAGCTTCACACCAACTTTGCCTCCTATATGC GAAAAGGGACCTCTGAAGATAATGTCCAGTACCAGGCCCATGTCTCTGAGTACCATCGCAATCCTGAATT CAGTCAAGAAAGCTGTTGCGTCTAAGAGCAGGACACGCAGTTTAGGAGCCCTGTCTTTCAACATGGAGAA CGGAAGCCCAGAAAATCCCTCCAGCCGTGCGTCTTCCTTGAGCTTGGAGCAGGAGTCTGAGAGAGCCGTC AAGCGTGTGACTAAAAGGGCGATCAGCACCCCAGAGAAGTCCATCAGCAATGTCACATTTGGAGCAGGGG TCAGTTACATAGGGACACCAGCAACTTCGTTTTTTACCAAAGAGAAACCTGAAGAAGCCCAGGAAGAAGG AAATGACATTTTATGTTTGTCATTGCTTGATAAAAGGCCTCCGCAGTCTCCACAGTGGAATGTGAAGTCT CCAGCCAGGAGCCAAGACAACCCCCGGTCCGGCAGAAGGTCCCTTCGCAAGGCCCCGCTGAAGACAGCCA ACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001034000
Insert Size	4485 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001034000.1, NP_001029172.1
RefSeq Size	5185 bp
RefSeq ORF	4485 bp
Locus ID	315508

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