Arhgef12 (NM_001013246) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Larg
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205717 representing NM_001013246 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCACACAGTCTACTATCACGGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTT TGAACCGAGAGTCACCAACAGATAAGAAACAGAAAGTCGAACGCAGCACATCACATGATTTTGACCCCAC AGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATCTATGGTCTTGTTCAG CGCTGTGTGATCATCCAGAAAGATGACAATGGATTCGGGCTGACAGTCAGCGGGGACAATCCAGTCTTCG TACAATCTGTCAAAGAAGATGGAGCAGCCATGCGCGCTGGAGTACAAACAGGTGATCGAATCATCAAGGT GAATGGGACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAACTAATCAGATCTGGTTCCTATGTA GCTCTCACTGTCCAGGGACGCCCACCTGGGTCGCCCCAGATTCCACTTGCTGAGTCTGAAGTAGAACCAT CAGTCATTGGACATATGTCTCCCATTATGACATCCCCTCATTCCCCTGGAGCAGCCGGGAACATGGAGAG AATCACTAGTCCTGTGCTCATGGGGGAGGAAAACAATGTGGTCCATAACCAGAAAGTAGAGATTCTGAGG AAAATGTTACAGAAAGAACAGGAGCGTCTGCAGTTACTGCAGGAAGATTACAACCGAACGGCTACCCAGA GATTGCTCAAGGAGATCCAGGAGGCCAAGAAGCACATCCCCCAGCTGCAGGAGCAGCTGTCCAAAGCCAC AGGCTCTGCTCAGGACGGAGCTGTAATTGCACCCTCCAGGCCATTAGGTGATGCCCTAACACTCAGCGAG GCAGAAACGGACCCTGGAGACGGCCTGTGCAGGACTGACTGGAGCAGTGGAGATGCTTCTCGGCCCAGTA GTGACAGTGCAGATAGCCCCAAGAGTAGCCTGAAGGAAAGGGTTTGTCTCGAGGACACCCCAGAGAGGAC TGAAGGAGTTCAGGATGCTGACACACAGTCGCTGGTCGGCAGTCCCTCCACCCGTGGCGCCCCTCATATT ATCGGAGCAGAGGATGATGATTTTGGTACTGAACACGAGCAGATCAATGGACAGTGCAGCTGTTTCCAGA GCATTGAGTTGCTGAAGTCTCGCCCTGCCCACTTGGCTGTTTTCTTACACCATGTAGTCTCACAGTTTGA CCCTGCAACATTGCTCTGTTATCTCTATTCAGACCTGTATAAGCAGACGAACTCCAAGGAGACTCGGCGG GTCTTCCTGGAGTTTCATCAGTTCTTCTTGGACCGATCTGCACACCTGAGAGTTTCCGTCCCCGAGGAAA TCTCTGTGGATCTAGAAAAGAGAAGACCCGAGCTCATTCCTGAGGATCTGCATCGCCATTACATTCAAAC AATGCAGGAGAGGGTTCACCCTGAAGTTCAGAGGCACTTGGAGGATTTTCGACAGAAACGCAGCATGGGA CTGACCTTGGCTGAGAGTGAGCTGACCAAACTTGATGCAGAGAGAGACAAGGACCGGGGAACTCTGGAGA AGGAGCGAGCATGTGCGGAACAGATCGTTACCAAAATTGAAGAAGTGTTGATGACTGCTCAGGCTGTAGA AGAAGAAAGGAGTTCCACAATGCAGTATGTAATTCTCATGTATATGAAGTATTTGGGAGTAAAAGTGAAA GAACCTCGAAACTTGGAGCACAAGCGGGGTCGGATCGGCTTCCTTCCAAAGATCAAGCAAAGCATGAAGA AAGATAGAGAGGGCGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATTTTGGGACCCCCAAGGAGACC AAGCCGCCACGACAACAGTGCAATTGGCAGGGCCATGGAGATACAGAAGCTTCGCCACCCGAAGCACTTG TCCGCACCCTCATCTATGAGTCCTGAGCTTCAGGACCCTGCTAAGTTGCGCCAGAGTGGAGTAGCCAATG AAGGAACAGACACTGGATATCTGCCTGCCAGTTCCATGTCCTCTGCCACCTCAGGGGCTGCTCTTTCCCA AGAGGGAGGGAGAGAGAATGACACAGGAGCAAAACAAGTTGGAGACGCGGTGGCACCTGGAGACTGCTTG GATAGCATACCTCGAGTCCCCAATACTGTTTTTGACTTCCCCCCGCCTCTGCTAGATCAAGTGCAGGAGG AGGAATGTGAAGTGGAGAGGGTGGCAGAACATGGAACCCCAAAACCCTTTCGAAAGTTTGACAGCATCGC CTTTGGAGAAAGTCAGAGTGAGGACGAGCAGTTTGAAAATGACCTGGAAACAGATCCACCCAACTGGCAG CAGCTCGTTAGCAGAGAAGTGCTACTGGGACTGAAACCCTCCGAGATCAAGAGACAGGAAGTGATTAATG AATTGTTCTACACTGAGAGAGCGCATGTCCGAACACTGAAGGTTCTTGACCAAGTATTCTATCAGCGAGT GTCCAGGGAAGGGATTCTATCACCTTCCGAGCTGCGCAAGATTTTTTCAAACTTGGAGGATATCCTTCAA CTTCATGTTGGGTTGAATGAGCAGATGAAAGCTGTCCGAAAAAGGAACGAGACCTCAGTTATTGATCATA TTGGGGAAGATCTGCTCCTTTGGTTCAGTGGACCAGGGGAGGAGAAGCTGAAGCACGCTGCTGCCACATT CTGCAGTAACCAGCCTTTCGCCCTGGAAATGATCAAGTCTCGCCAGAAAAAGGATTCTCGATTCCAGACT TTTGTGCAAGATGCTGAGAGTAACCCCCTGTGCCGTCGTCTTCAGCTGAAGGATATCATTCCCACCCAAA TGCAGAGGCTCACCAAGTACCCCCTTCTTTTGGATAATATCGCCAAATACACAGAATGGCCTCCAGAAAG GGAAAAGGTGAAGAAGGCTGCAGATCAATGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAGGGAG GCAGAAAACAAGCAGCGCTTAGAAGACTATCAGCGTCGCCTTGACACCTCCAGTCTCAAGTTGTCTGAGT ACCCGAATGTTGATGAGCTCAGGAATTTGGATTTAACAAAAAGAAAGATGATTCATGAAGGGCCGTTGGT CTGGAAGGTGAATAGAGATAAAAGCATCGATCTGTATACACTGCTGCTGGAAGACATTCTTGTGCTGTTA CAGAAGCAGGACGACAGGCTGGTACTGAGGTGCCACAGCAAGATCCTGGCCTCCACAGCTGACAGCAAGC ACACCTTCAGTCCTGTCATTAAGCTCAGCACAGTGCTGGTCCGACAGGTGGCCACAGATAACAAAGCTTT ATTCGTCATCTCCATGTCAGATAATGGAGCCCAAATTTATGAATTGGTAGCACAGACGGTTTCTGAAAAG ACGGTCTGGCAAGACCTCATCTGTCGGATGGCCGCATCAGTGAAGGAGCAGTCCACAAAGCCGATTCCCC TCCCACAGCCGCCACCTTGCGAAGGAGACAATGATGAAGAAGAACCAGCCAAGTTAAAAGTGGAGCACCA TGATCTTTCTGTCGCTGGTCTGCAGAGTCCAGACAGAGTTTTGGGATTAGAATCTCCCTTAATTTCGTCA AAACCTCAGTCCCATTCAATGAATACCCCTGGCAAATCAGAGGCAGAGGCAGAACATCTCTTTGTGACCG CAACACAGTTTGCAAAGGAGCAGCATGCAAATGGGACCTTCAAGGAAGGTGATGGCGGGTATCCAGTCAC AATCTCAGATCCACACTTGCCCGTGTCAGAGGAGCGGTGGGCATTGGACGCACTGAGAAATTTGGGCTTG TTGAAGCAGTTGCTGGTTCAGCAGTTGGGGCTCACGGAGAAGACTACTCAGGAAGACTGGCGGTCCTTCT CAAGATACAGACCGGCTTCTGAAGAGGCACAGGCGGACAGTGGCATTCCGGACTTGGAAAGTGTTAAGGC TTGTCATGCCGGGGAAGGACAGATGTCTTTTAAAACTGGAACTGGTGATGTTGCAACTTGTGACAGTCCA CGGACTTCAACTGAGTCCTGTGCTGTACAGGATTCGGTGATACTGGCTTCCCAAGACAGCCAGGCAAGCA ACATCATAGAAATGGACCACATGCTCCTGACCCCAGAGGTGCCTACTACTGCAGAGCCAGAGGGGGGTCT GGATGACAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGCGATGATAATCCATCTGAAGGTGAT GGCGCAGTCAGAAAGGAAGAGGAGGATGTTAATGTGCGCATCTCAGGAAACTGTTTGATCCTTGATGGCT ATGATGCAGTGCAGGAGAGCTCCACAGATGAGGAGATTGCCTCCTCATTCACCCTGCAGCCTGTGACAGG CCTCCCTTCTGTGGACTCCAGCCACCAGCAGCAACACTCCCCTCAGAATGCCATTTCCGAGGGGGCGATT TCACCATCCACCCCAGAATTCCTGGTCCAGCGACGCTGGAGAGCTGTGGATGCTTGCTCTGAGGTCCAGA GTCCCTCTGCTTGGACAGATTCACAAAGCCAAATCCTGGAGTACATTCATAAAATAGAGGCTGACCTGGA ACACTTAAAGAAGGTGGAGGAAAGTTACACCCTTCTTTGCCAAAGGCTGGCTGGCTCAGCCCTCCCCGAC AAGGTCTCAGATAAAAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001013246
Insert Size	4641 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001013246.1, NP_001013264.1
RefSeq Size	4668 bp
RefSeq ORF	4641 bp
Locus ID	367072

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.