Arhgef12 (NM_001013246) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Larg
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205717 representing NM_001013246 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCACACAGTCTACTATCACGGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTT TGAACCGAGAGTCACCAACAGATAAGAAACAGAAAGTCGAACGCAGCACATCACATGATTTTGACCCCAC AGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATCTATGGTCTTGTTCAG CGCTGTGTGATCATCCAGAAAGATGACAATGGATTCGGGCTGACAGTCAGCGGGGACAATCCAGTCTTCG TACAATCTGTCAAAGAAGATGGAGCAGCCATGCGCGCTGGAGTACAAACAGGTGATCGAATCATCAAGGT GAATGGGACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAACTAATCAGATCTGGTTCCTATGTA GCTCTCACTGTCCAGGGACGCCCACCTGGGTCGCCCCAGATTCCACTTGCTGAGTCTGAAGTAGAACCAT CAGTCATTGGACATATGTCTCCCATTATGACATCCCCTCATTCCCCTGGAGCAGCCGGGAACATGGAGAG AATCACTAGTCCTGTGCTCATGGGGGAGGAAAACAATGTGGTCCATAACCAGAAAGTAGAGATTCTGAGG AAAATGTTACAGAAAGAACAGGAGCGTCTGCAGTTACTGCAGGAAGATTACAACCGAACGGCTACCCAGA GATTGCTCAAGGAGATCCAGGAGGCCAAGAAGCACATCCCCCAGCTGCAGGAGCAGCTGTCCAAAGCCAC AGGCTCTGCTCAGGACGGAGCTGTAATTGCACCCTCCAGGCCATTAGGTGATGCCCTAACACTCAGCGAG GCAGAAACGGACCCTGGAGACGGCCTGTGCAGGACTGACTGGAGCAGTGGAGATGCTTCTCGGCCCAGTA GTGACAGTGCAGATAGCCCCAAGAGTAGCCTGAAGGAAAGGGTTTGTCTCGAGGACACCCCAGAGAGGAC TGAAGGAGTTCAGGATGCTGACACACAGTCGCTGGTCGGCAGTCCCTCCACCCGTGGCGCCCCTCATATT ATCGGAGCAGAGGATGATGATTTTGGTACTGAACACGAGCAGATCAATGGACAGTGCAGCTGTTTCCAGA GCATTGAGTTGCTGAAGTCTCGCCCTGCCCACTTGGCTGTTTTCTTACACCATGTAGTCTCACAGTTTGA CCCTGCAACATTGCTCTGTTATCTCTATTCAGACCTGTATAAGCAGACGAACTCCAAGGAGACTCGGCGG GTCTTCCTGGAGTTTCATCAGTTCTTCTTGGACCGATCTGCACACCTGAGAGTTTCCGTCCCCGAGGAAA TCTCTGTGGATCTAGAAAAGAGAAGACCCGAGCTCATTCCTGAGGATCTGCATCGCCATTACATTCAAAC AATGCAGGAGAGGGTTCACCCTGAAGTTCAGAGGCACTTGGAGGATTTTCGACAGAAACGCAGCATGGGA CTGACCTTGGCTGAGAGTGAGCTGACCAAACTTGATGCAGAGAGAGACAAGGACCGGGGAACTCTGGAGA AGGAGCGAGCATGTGCGGAACAGATCGTTACCAAAATTGAAGAAGTGTTGATGACTGCTCAGGCTGTAGA AGAAGAAAGGAGTTCCACAATGCAGTATGTAATTCTCATGTATATGAAGTATTTGGGAGTAAAAGTGAAA GAACCTCGAAACTTGGAGCACAAGCGGGGTCGGATCGGCTTCCTTCCAAAGATCAAGCAAAGCATGAAGA AAGATAGAGAGGGCGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATTTTGGGACCCCCAAGGAGACC AAGCCGCCACGACAACAGTGCAATTGGCAGGGCCATGGAGATACAGAAGCTTCGCCACCCGAAGCACTTG TCCGCACCCTCATCTATGAGTCCTGAGCTTCAGGACCCTGCTAAGTTGCGCCAGAGTGGAGTAGCCAATG AAGGAACAGACACTGGATATCTGCCTGCCAGTTCCATGTCCTCTGCCACCTCAGGGGCTGCTCTTTCCCA AGAGGGAGGGAGAGAGAATGACACAGGAGCAAAACAAGTTGGAGACGCGGTGGCACCTGGAGACTGCTTG GATAGCATACCTCGAGTCCCCAATACTGTTTTTGACTTCCCCCCGCCTCTGCTAGATCAAGTGCAGGAGG AGGAATGTGAAGTGGAGAGGGTGGCAGAACATGGAACCCCAAAACCCTTTCGAAAGTTTGACAGCATCGC CTTTGGAGAAAGTCAGAGTGAGGACGAGCAGTTTGAAAATGACCTGGAAACAGATCCACCCAACTGGCAG CAGCTCGTTAGCAGAGAAGTGCTACTGGGACTGAAACCCTCCGAGATCAAGAGACAGGAAGTGATTAATG AATTGTTCTACACTGAGAGAGCGCATGTCCGAACACTGAAGGTTCTTGACCAAGTATTCTATCAGCGAGT GTCCAGGGAAGGGATTCTATCACCTTCCGAGCTGCGCAAGATTTTTTCAAACTTGGAGGATATCCTTCAA CTTCATGTTGGGTTGAATGAGCAGATGAAAGCTGTCCGAAAAAGGAACGAGACCTCAGTTATTGATCATA TTGGGGAAGATCTGCTCCTTTGGTTCAGTGGACCAGGGGAGGAGAAGCTGAAGCACGCTGCTGCCACATT CTGCAGTAACCAGCCTTTCGCCCTGGAAATGATCAAGTCTCGCCAGAAAAAGGATTCTCGATTCCAGACT TTTGTGCAAGATGCTGAGAGTAACCCCCTGTGCCGTCGTCTTCAGCTGAAGGATATCATTCCCACCCAAA TGCAGAGGCTCACCAAGTACCCCCTTCTTTTGGATAATATCGCCAAATACACAGAATGGCCTCCAGAAAG GGAAAAGGTGAAGAAGGCTGCAGATCAATGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAGGGAG GCAGAAAACAAGCAGCGCTTAGAAGACTATCAGCGTCGCCTTGACACCTCCAGTCTCAAGTTGTCTGAGT ACCCGAATGTTGATGAGCTCAGGAATTTGGATTTAACAAAAAGAAAGATGATTCATGAAGGGCCGTTGGT CTGGAAGGTGAATAGAGATAAAAGCATCGATCTGTATACACTGCTGCTGGAAGACATTCTTGTGCTGTTA CAGAAGCAGGACGACAGGCTGGTACTGAGGTGCCACAGCAAGATCCTGGCCTCCACAGCTGACAGCAAGC ACACCTTCAGTCCTGTCATTAAGCTCAGCACAGTGCTGGTCCGACAGGTGGCCACAGATAACAAAGCTTT ATTCGTCATCTCCATGTCAGATAATGGAGCCCAAATTTATGAATTGGTAGCACAGACGGTTTCTGAAAAG ACGGTCTGGCAAGACCTCATCTGTCGGATGGCCGCATCAGTGAAGGAGCAGTCCACAAAGCCGATTCCCC TCCCACAGCCGCCACCTTGCGAAGGAGACAATGATGAAGAAGAACCAGCCAAGTTAAAAGTGGAGCACCA TGATCTTTCTGTCGCTGGTCTGCAGAGTCCAGACAGAGTTTTGGGATTAGAATCTCCCTTAATTTCGTCA AAACCTCAGTCCCATTCAATGAATACCCCTGGCAAATCAGAGGCAGAGGCAGAACATCTCTTTGTGACCG CAACACAGTTTGCAAAGGAGCAGCATGCAAATGGGACCTTCAAGGAAGGTGATGGCGGGTATCCAGTCAC AATCTCAGATCCACACTTGCCCGTGTCAGAGGAGCGGTGGGCATTGGACGCACTGAGAAATTTGGGCTTG TTGAAGCAGTTGCTGGTTCAGCAGTTGGGGCTCACGGAGAAGACTACTCAGGAAGACTGGCGGTCCTTCT CAAGATACAGACCGGCTTCTGAAGAGGCACAGGCGGACAGTGGCATTCCGGACTTGGAAAGTGTTAAGGC TTGTCATGCCGGGGAAGGACAGATGTCTTTTAAAACTGGAACTGGTGATGTTGCAACTTGTGACAGTCCA CGGACTTCAACTGAGTCCTGTGCTGTACAGGATTCGGTGATACTGGCTTCCCAAGACAGCCAGGCAAGCA ACATCATAGAAATGGACCACATGCTCCTGACCCCAGAGGTGCCTACTACTGCAGAGCCAGAGGGGGGTCT GGATGACAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGCGATGATAATCCATCTGAAGGTGAT GGCGCAGTCAGAAAGGAAGAGGAGGATGTTAATGTGCGCATCTCAGGAAACTGTTTGATCCTTGATGGCT ATGATGCAGTGCAGGAGAGCTCCACAGATGAGGAGATTGCCTCCTCATTCACCCTGCAGCCTGTGACAGG CCTCCCTTCTGTGGACTCCAGCCACCAGCAGCAACACTCCCCTCAGAATGCCATTTCCGAGGGGGCGATT TCACCATCCACCCCAGAATTCCTGGTCCAGCGACGCTGGAGAGCTGTGGATGCTTGCTCTGAGGTCCAGA GTCCCTCTGCTTGGACAGATTCACAAAGCCAAATCCTGGAGTACATTCATAAAATAGAGGCTGACCTGGA ACACTTAAAGAAGGTGGAGGAAAGTTACACCCTTCTTTGCCAAAGGCTGGCTGGCTCAGCCCTCCCCGAC AAGGTCTCAGATAAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205717 representing NM_001013246 Red=Cloning site Green=Tags(s) MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDFDPTDSSSKKTKSSSEESRSEIYGLVQ RCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIRSGSYV ALTVQGRPPGSPQIPLAESEVEPSVIGHMSPIMTSPHSPGAAGNMERITSPVLMGEENNVVHNQKVEILR KMLQKEQERLQLLQEDYNRTATQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVIAPSRPLGDALTLSE AETDPGDGLCRTDWSSGDASRPSSDSADSPKSSLKERVCLEDTPERTEGVQDADTQSLVGSPSTRGAPHI IGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRR VFLEFHQFFLDRSAHLRVSVPEEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMG LTLAESELTKLDAERDKDRGTLEKERACAEQIVTKIEEVLMTAQAVEEERSSTMQYVILMYMKYLGVKVK EPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMEIQKLRHPKHL SAPSSMSPELQDPAKLRQSGVANEGTDTGYLPASSMSSATSGAALSQEGGRENDTGAKQVGDAVAPGDCL DSIPRVPNTVFDFPPPLLDQVQEEECEVERVAEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQ QLVSREVLLGLKPSEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQ LHVGLNEQMKAVRKRNETSVIDHIGEDLLLWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQT FVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPPEREKVKKAADQCRQILNYVNQAVRE AENKQRLEDYQRRLDTSSLKLSEYPNVDELRNLDLTKRKMIHEGPLVWKVNRDKSIDLYTLLLEDILVLL QKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEK TVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPAKLKVEHHDLSVAGLQSPDRVLGLESPLISS KPQSHSMNTPGKSEAEAEHLFVTATQFAKEQHANGTFKEGDGGYPVTISDPHLPVSEERWALDALRNLGL LKQLLVQQLGLTEKTTQEDWRSFSRYRPASEEAQADSGIPDLESVKACHAGEGQMSFKTGTGDVATCDSP RTSTESCAVQDSVILASQDSQASNIIEMDHMLLTPEVPTTAEPEGGLDDSGEHFFDAREAHSDDNPSEGD GAVRKEEEDVNVRISGNCLILDGYDAVQESSTDEEIASSFTLQPVTGLPSVDSSHQQQHSPQNAISEGAI SPSTPEFLVQRRWRAVDACSEVQSPSAWTDSQSQILEYIHKIEADLEHLKKVEESYTLLCQRLAGSALPD KVSDKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001013246
ORF Size	4638 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001013246.1, NP_001013264.1
RefSeq Size	4668 bp
RefSeq ORF	4641 bp
Locus ID	367072
MW	172.7 kDa

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