Rock1 (NM_031098) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	P160Rock; ROCK-I
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205715 representing NM_031098 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACTGGGGACAGTTTTGAGACTCGGTTTGAAAAAATCGACAACCTGCTGCGGGATCCCAAATCGG AAGTGAATTCAGATTGTTTGCTGGATGGATTGGATGCTTTGGTTTATGATTTGGATTTTCCTGCCTTAAG AAAAAACAAAAATATTGACAACTTTCTAAGCAGATATAAAGACACAATAAATAAAATTAGAGATTTACGG ATGAAAGCTGAAGATTATGAAGTAGTAAAGGTAATCGGCAGAGGTGCATTTGGAGAAGTTCAATTGGTAA GGCATAAATCCACCAGGAAGGTTTATGCTATGAAGCTTCTCAGCAAATTTGAAATGATTAAGAGATCTGA TTCTGCTTTTTTCTGGGAAGAAAGGGACATCATGGCATTTGCCAATAGTCCTTGGGTTGTTCAGCTTTTT TATGCATTCCAAGATGACCGATATCTCTATATGGTGATGGAGTACATGCCTGGTGGAGACCTTGTAAACC TGATGAGCAACTACGATGTGCCTGAAAAGTGGGCACGATTCTATACTGCAGAAGTAGTTCTCGCATTGGA TGCRATCCATTCCATGGGTTTTATTCATAGAGATGTGAAGCCTGACAACATGCTGCTGGATAAGTCTGGA CATTTGAAGTTAGCCGACTTTGGTACTTGTATGAAGATGAATAAGGAAGGTATGGTACGATGTGATACAG CAGTTGGAACACCTGATTATATTTCACCTGAAGTATTAAAATCACAAGGCGGTGATGGCTATTATGGACG AGAGTGTGACTGGTGGTCAGTTGGTGTATTTTTATATGAAATGCTTGTAGGTGATACACCTTTTTATGCA GATTCTTTGGTTGGGACGTACAGTAAAATTATGAACCATAAAAATTCACTTACTTTCCCTGATGATAATG ACATATCAAAAGAGGCGAAAAATCTCATTTGTGCCTTCCTTACGGACAGAGAAGTGAGATTGGGCAGAAA TGGTGTAGAGGAAATCAAACGTCATCTCTTCTTCAAAAATGATCAGTGGGCTTGGGAAACGCTCCGAGAC ACTGTAGCACCAGTTGTGCCTGATTTAAGTAGTGACATTGATACTAGTAATTTTGATGACTTAGAAGAAG ATAAAGGAGATGAAGAAACATTTCCTATTCCAAAAGCTTTTGTTGGCAATCAGCTACCTTTTGTAGGATT TACATATTATAGCAATCGTAGATACTTACCTTCAGCAAATCCCAGTGAGAACAGATCTAGCTCCAATGTG GATAAAAACGTGCAGGAAAGTTTACAGAAAACAATCTACAAGCTTGAAGAACAACTGCATAATGAAATGC AATTAAAAGATGAAATGGAGCAAAAGTGCAGAACTTCAAACATAAAACTAGACAAGATAATGAAAGAATT GGATGAAGAGGGGAATCAAAGAAGAAATCTAGAATCTGCGGTGTCTCAAATTGAGAAAGAAAAGATGTTG CTACAACATAGAATTAATGAGTACCAAAGAAAAGTGGAACAGGAAAATGAGAAAAGAAGAAATGTAGAAA ATGAAGTTTCTACACTAAAGGACCAGTTGGAAGACTTACGGAAAGCAAGTCAGAGCTCACAGCTTGCTAA TGAGAAGCTGACACAGTTACAGAAGCAGTTAGAAGAAGCGAATGACTTACTTAGGACAGAATCAGACACA GCTGTAAGATTAAGGAAGAGTCACACAGAGATGAGCAAGTCAGTTAGTCAGCTGGAGTCTCTGAACAGAG AGTTACAGGAGAGAAACAGAATGTTAGAGAATTCCAAGTCACAAGCAGACAAGGATTACTACCAGCTGCA AGCTGTATTGGAAGCTGAGCGAAGAGACAGAGGCCATGATTCTGAGATGATTGGAGACCTTCAAGCACGA ATTACGTCTTTACAAGAAGAGGTGAAGCATCTCAAACATAATCTTGAAAGAGTAGAAGGAGAAAGAAAAG AGGCTCAAGACATGCTCAATCATTCAGAAAAGGAAAAAAATAATTTGGAGATTGATTTGAACTACAAGCT CAAGTCTATTCAGCAGAGGTTAGAACAAGAAGTAAATGAACACAAAGTAACCAAAGCGCGGCTAACTGAC AAGCATCAGTCTATTGAAGAGGCAAAGTCTGTTGCCATGTGTGAAATGGAAAAAAAGCTGAAGGAAGAGA GAGAAGCTCGAGAGAAGGCTGAAAATAGAGTTGTTGAGACTGAGAAGCAATGTTCTATGCTAGATGTTGA CCTGAAACAGTCTCAGCAGAAGCTAGAACATTTGACCGAAAATAAAGAAAGGCTGGAGGATGCAGTTAAG AGTCTCACCCTGCAGCTGGAACAGGAATCCAATAAGCGGATCTTGCTACAGAGTGAACTGAAGACTCAAG CGTTTGAAGCAGACAATTTAAAAGGGTTAGAAAAACAGATGAAACAGGAAATAAATACTTTATTGGAAGC TAAAAGATTGTTAGAATTTGAGTTAGCTCAGCTTACAAAGCAATACAGAGGAAATGAAGGGCAAATGCGG GAGTTACAAGATCAGCTTGAAGCTGAGCAATATTTCTCTACACTTTATAAAACCCAGGTAAAGGAACTTA AAGAAGAAATTGAAGAAAAAAACAGAGAAAATTTAAGGAAAATACAGGAGCTGCAAAGTGAAAAAGAAAC TCTTTCGACTCAGTTGGATTTAGCAGAAACAAAAGCTGAGTCTGAGCAGTTGGCTCGAGGAATTCTGGAA GAACAGTATTTTGAGTTGACTCAAGAAAGCAAGAAAGCTGCTTCAAGAAATAGACAAGAGATTACAGATA AAGATCATACCGTTAGTCGGCTTGAAGAAGCAAACAATGCGCTAACCAAAGATATTGAACTACTAAGAAA AGAAAATGAAGAGCTAAATGAAAGGATGAGAACAGCAGAAGAAGAATATAAATTGAAGAAGGAGGAAGAA ATAAGTAATCTTAAGGCTGCCTTTGAAAAGAATATCAGTACCGAACGGACCCTTAAAACACAGGCTGTTA ACAAGTTGGCAGAAATAATGAATCGCAAAGATTTTAAAATTGATAGGAAGAAAGCTAATACTCAAGATTT GAGAAAAAAAGAAAAGGAAAATAGAAAGCTCCAACTAGAACTTAACCAAGAAAGAGAAAAATTTAACCAA ATGGTGGTGAAACACCAGAAGGAGCTGAATGACATGCAAGCGCAATTGGTAGAAGAATGTACACATAGAA ATGAACTTCAAATGCAGTTGGCTAGCAAAGAGAGTGATATTGAGCAGTTGCGTGCAAAACTTTTGGACCT TTCGGATTCAACTAGTGTTGCTAGTTTTCCCAGTGCTGATGAGACTGACGGAAATCTTCCAGTTGGTTCT GCCTGCATTCCTTATTTATTTATCTTCTACTCTTCTAGTTCAAGAATTGAAGGTTGGCTTTCTGTACCAA ATAGAGGAAATATCAAAAGATATGGCTGGAAGAAACAGTATGTTGTGGTGAGCAGCAAAAAAATGTTGTT CTATAATGATGAGCAAGATAAGGAACAATCCAGTCCATCTATGGTGTTGGACATAGATAAATTGTTCCAT GTCCGACCTGTAACCCAAGGAGATGTGTACAGAGCTGAAACTGAAGAAATTCCTAAAATATTCCAGATAC TGTATGCAAATGAAGGTGAATGTAGAAAAGATATAGAAGTGGAACCAGTTCAACAAGGTGAAAAAACTAA TTTCCAGAATCACAAAGGCCATGAATTCATTCCTACCCTCTACCACTTTCCAGCCAACTGCGAGGCCTGT GCCAAACCTTTGTGGCATGTTTTTAAGCCACCGCCTGCCCTAGAGTGTCGAAGATGCCATGTTAAGTCCC ACAGGGACCATCTAGACAAGAAAGAGGACTTGATTCCCCCATGCAAAGTAAGTTATGATGTAACATCAGC AAGAGATATGTTGCTGTTAGCATGTCCTCAGGATGAACAAAAGAAATGGGTAACTCATCTGGTAAAGAAG ATTCCTAAGAAGGCACCTTCTGGCTTTGTCCGTGCCTCTCCTCGAACGCTGTCGACAAGATCTACTGCAA ATCAGTCTTTCCGGAAAGTAGTCAAAAATACATCTGGAAAAACTAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031098
Insert Size	4110 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031098.1, NP_112360.1
RefSeq Size	4501 bp
RefSeq ORF	4110 bp
Locus ID	81762
UniProt ID	Q63644
Gene Summary	protein serine/threonine kinase; involved in intracellular signaling [RGD, Feb 2006]

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