Rock1 (NM_031098) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	P160Rock; ROCK-I
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205715 representing NM_031098 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACTGGGGACAGTTTTGAGACTCGGTTTGAAAAAATCGACAACCTGCTGCGGGATCCCAAATCGG AAGTGAATTCAGATTGTTTGCTGGATGGATTGGATGCTTTGGTTTATGATTTGGATTTTCCTGCCTTAAG AAAAAACAAAAATATTGACAACTTTCTAAGCAGATATAAAGACACAATAAATAAAATTAGAGATTTACGG ATGAAAGCTGAAGATTATGAAGTAGTAAAGGTAATCGGCAGAGGTGCATTTGGAGAAGTTCAATTGGTAA GGCATAAATCCACCAGGAAGGTTTATGCTATGAAGCTTCTCAGCAAATTTGAAATGATTAAGAGATCTGA TTCTGCTTTTTTCTGGGAAGAAAGGGACATCATGGCATTTGCCAATAGTCCTTGGGTTGTTCAGCTTTTT TATGCATTCCAAGATGACCGATATCTCTATATGGTGATGGAGTACATGCCTGGTGGAGACCTTGTAAACC TGATGAGCAACTACGATGTGCCTGAAAAGTGGGCACGATTCTATACTGCAGAAGTAGTTCTCGCATTGGA TGCRATCCATTCCATGGGTTTTATTCATAGAGATGTGAAGCCTGACAACATGCTGCTGGATAAGTCTGGA CATTTGAAGTTAGCCGACTTTGGTACTTGTATGAAGATGAATAAGGAAGGTATGGTACGATGTGATACAG CAGTTGGAACACCTGATTATATTTCACCTGAAGTATTAAAATCACAAGGCGGTGATGGCTATTATGGACG AGAGTGTGACTGGTGGTCAGTTGGTGTATTTTTATATGAAATGCTTGTAGGTGATACACCTTTTTATGCA GATTCTTTGGTTGGGACGTACAGTAAAATTATGAACCATAAAAATTCACTTACTTTCCCTGATGATAATG ACATATCAAAAGAGGCGAAAAATCTCATTTGTGCCTTCCTTACGGACAGAGAAGTGAGATTGGGCAGAAA TGGTGTAGAGGAAATCAAACGTCATCTCTTCTTCAAAAATGATCAGTGGGCTTGGGAAACGCTCCGAGAC ACTGTAGCACCAGTTGTGCCTGATTTAAGTAGTGACATTGATACTAGTAATTTTGATGACTTAGAAGAAG ATAAAGGAGATGAAGAAACATTTCCTATTCCAAAAGCTTTTGTTGGCAATCAGCTACCTTTTGTAGGATT TACATATTATAGCAATCGTAGATACTTACCTTCAGCAAATCCCAGTGAGAACAGATCTAGCTCCAATGTG GATAAAAACGTGCAGGAAAGTTTACAGAAAACAATCTACAAGCTTGAAGAACAACTGCATAATGAAATGC AATTAAAAGATGAAATGGAGCAAAAGTGCAGAACTTCAAACATAAAACTAGACAAGATAATGAAAGAATT GGATGAAGAGGGGAATCAAAGAAGAAATCTAGAATCTGCGGTGTCTCAAATTGAGAAAGAAAAGATGTTG CTACAACATAGAATTAATGAGTACCAAAGAAAAGTGGAACAGGAAAATGAGAAAAGAAGAAATGTAGAAA ATGAAGTTTCTACACTAAAGGACCAGTTGGAAGACTTACGGAAAGCAAGTCAGAGCTCACAGCTTGCTAA TGAGAAGCTGACACAGTTACAGAAGCAGTTAGAAGAAGCGAATGACTTACTTAGGACAGAATCAGACACA GCTGTAAGATTAAGGAAGAGTCACACAGAGATGAGCAAGTCAGTTAGTCAGCTGGAGTCTCTGAACAGAG AGTTACAGGAGAGAAACAGAATGTTAGAGAATTCCAAGTCACAAGCAGACAAGGATTACTACCAGCTGCA AGCTGTATTGGAAGCTGAGCGAAGAGACAGAGGCCATGATTCTGAGATGATTGGAGACCTTCAAGCACGA ATTACGTCTTTACAAGAAGAGGTGAAGCATCTCAAACATAATCTTGAAAGAGTAGAAGGAGAAAGAAAAG AGGCTCAAGACATGCTCAATCATTCAGAAAAGGAAAAAAATAATTTGGAGATTGATTTGAACTACAAGCT CAAGTCTATTCAGCAGAGGTTAGAACAAGAAGTAAATGAACACAAAGTAACCAAAGCGCGGCTAACTGAC AAGCATCAGTCTATTGAAGAGGCAAAGTCTGTTGCCATGTGTGAAATGGAAAAAAAGCTGAAGGAAGAGA GAGAAGCTCGAGAGAAGGCTGAAAATAGAGTTGTTGAGACTGAGAAGCAATGTTCTATGCTAGATGTTGA CCTGAAACAGTCTCAGCAGAAGCTAGAACATTTGACCGAAAATAAAGAAAGGCTGGAGGATGCAGTTAAG AGTCTCACCCTGCAGCTGGAACAGGAATCCAATAAGCGGATCTTGCTACAGAGTGAACTGAAGACTCAAG CGTTTGAAGCAGACAATTTAAAAGGGTTAGAAAAACAGATGAAACAGGAAATAAATACTTTATTGGAAGC TAAAAGATTGTTAGAATTTGAGTTAGCTCAGCTTACAAAGCAATACAGAGGAAATGAAGGGCAAATGCGG GAGTTACAAGATCAGCTTGAAGCTGAGCAATATTTCTCTACACTTTATAAAACCCAGGTAAAGGAACTTA AAGAAGAAATTGAAGAAAAAAACAGAGAAAATTTAAGGAAAATACAGGAGCTGCAAAGTGAAAAAGAAAC TCTTTCGACTCAGTTGGATTTAGCAGAAACAAAAGCTGAGTCTGAGCAGTTGGCTCGAGGAATTCTGGAA GAACAGTATTTTGAGTTGACTCAAGAAAGCAAGAAAGCTGCTTCAAGAAATAGACAAGAGATTACAGATA AAGATCATACCGTTAGTCGGCTTGAAGAAGCAAACAATGCGCTAACCAAAGATATTGAACTACTAAGAAA AGAAAATGAAGAGCTAAATGAAAGGATGAGAACAGCAGAAGAAGAATATAAATTGAAGAAGGAGGAAGAA ATAAGTAATCTTAAGGCTGCCTTTGAAAAGAATATCAGTACCGAACGGACCCTTAAAACACAGGCTGTTA ACAAGTTGGCAGAAATAATGAATCGCAAAGATTTTAAAATTGATAGGAAGAAAGCTAATACTCAAGATTT GAGAAAAAAAGAAAAGGAAAATAGAAAGCTCCAACTAGAACTTAACCAAGAAAGAGAAAAATTTAACCAA ATGGTGGTGAAACACCAGAAGGAGCTGAATGACATGCAAGCGCAATTGGTAGAAGAATGTACACATAGAA ATGAACTTCAAATGCAGTTGGCTAGCAAAGAGAGTGATATTGAGCAGTTGCGTGCAAAACTTTTGGACCT TTCGGATTCAACTAGTGTTGCTAGTTTTCCCAGTGCTGATGAGACTGACGGAAATCTTCCAGTTGGTTCT GCCTGCATTCCTTATTTATTTATCTTCTACTCTTCTAGTTCAAGAATTGAAGGTTGGCTTTCTGTACCAA ATAGAGGAAATATCAAAAGATATGGCTGGAAGAAACAGTATGTTGTGGTGAGCAGCAAAAAAATGTTGTT CTATAATGATGAGCAAGATAAGGAACAATCCAGTCCATCTATGGTGTTGGACATAGATAAATTGTTCCAT GTCCGACCTGTAACCCAAGGAGATGTGTACAGAGCTGAAACTGAAGAAATTCCTAAAATATTCCAGATAC TGTATGCAAATGAAGGTGAATGTAGAAAAGATATAGAAGTGGAACCAGTTCAACAAGGTGAAAAAACTAA TTTCCAGAATCACAAAGGCCATGAATTCATTCCTACCCTCTACCACTTTCCAGCCAACTGCGAGGCCTGT GCCAAACCTTTGTGGCATGTTTTTAAGCCACCGCCTGCCCTAGAGTGTCGAAGATGCCATGTTAAGTCCC ACAGGGACCATCTAGACAAGAAAGAGGACTTGATTCCCCCATGCAAAGTAAGTTATGATGTAACATCAGC AAGAGATATGTTGCTGTTAGCATGTCCTCAGGATGAACAAAAGAAATGGGTAACTCATCTGGTAAAGAAG ATTCCTAAGAAGGCACCTTCTGGCTTTGTCCGTGCCTCTCCTCGAACGCTGTCGACAAGATCTACTGCAA ATCAGTCTTTCCGGAAAGTAGTCAAAAATACATCTGGAAAAACTAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205715 representing NM_031098 Red=Cloning site Green=Tags(s) MSTGDSFETRFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLR MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLF YAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDXIHSMGFIHRDVKPDNMLLDKSG HLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYA DSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRD TVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANPSENRSSSNV DKNVQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESAVSQIEKEKML LQHRINEYQRKVEQENEKRRNVENEVSTLKDQLEDLRKASQSSQLANEKLTQLQKQLEEANDLLRTESDT AVRLRKSHTEMSKSVSQLESLNRELQERNRMLENSKSQADKDYYQLQAVLEAERRDRGHDSEMIGDLQAR ITSLQEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSIQQRLEQEVNEHKVTKARLTD KHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVETEKQCSMLDVDLKQSQQKLEHLTENKERLEDAVK SLTLQLEQESNKRILLQSELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMR ELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLRKIQELQSEKETLSTQLDLAETKAESEQLARGILE EQYFELTQESKKAASRNRQEITDKDHTVSRLEEANNALTKDIELLRKENEELNERMRTAEEEYKLKKEEE ISNLKAAFEKNISTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQ MVVKHQKELNDMQAQLVEECTHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPVGS ACIPYLFIFYSSSSRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKMLFYNDEQDKEQSSPSMVLDIDKLFH VRPVTQGDVYRAETEEIPKIFQILYANEGECRKDIEVEPVQQGEKTNFQNHKGHEFIPTLYHFPANCEAC AKPLWHVFKPPPALECRRCHVKSHRDHLDKKEDLIPPCKVSYDVTSARDMLLLACPQDEQKKWVTHLVKK IPKKAPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031098
ORF Size	4107 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031098.1, NP_112360.1
RefSeq Size	4501 bp
RefSeq ORF	4110 bp
Locus ID	81762
UniProt ID	Q63644
MW	159.7 kDa
Gene Summary	protein serine/threonine kinase; involved in intracellular signaling [RGD, Feb 2006]

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