Ace (NM_012544) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CD143; Dcp1; StsRR92
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205616 representing NM_012544 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGCGTCCGGCCAGCGGGGGCGGTGGCCGTTGTCACCGCCGCTCTTGATGCTGTCGCTGCTGC TGCTGCTGCTGCTGCCGCCGTCGCCCGCCCCGGCGCTTGACCCTGGATTGCAGCCGGGCAACTTTTCCGC GGACGAGGCAGGGGCGCAGCTCTTCGCTGACAGCTATAACTCGAGTGCCGAGGTGGTGATGTTCCAGAGC ACCGCAGCCAGCTGGGCGCACGACACCAACATCACGGAGGAGAATGCGCGGCTCCAGGAGGAAGCGGCCC TGATCAACCAGGAGTTTGCAGAGGTCTGGGGCAAGAAGGCCAAGGAGCTGTATGAGTCCATCTGGCAGAA CTTCACTGACCAAAAGCTGCGAAGGATCATCGGATCCGTACAGACCCTAGGACCTGCCAACCTGCCCCTG ACCCAGCGGCTGCAGTACAACTCTCTGCTAAGCAACATGAGCAGAATCTACTCCACCGGCAAGGTCTGCT TCCCCAACAAGACTGCCACCTGCTGGTCCCTGGACCCAGAGCTCACCAACATCCTGGCTTCCTCACGAAA CTATGCCAAGGTGCTGTTTGCCTGGGAAGGCTGGCATGATGCTGTGGGTATCCCACTGAAGCCCCTCTAT CAGGACTTTACTGCCCTCAGTAATGAAGCCTACAGACAAGATGGCTTCTCAGACACAGGAGCCTACTGGC GCTCCTGGTATGAGTCCCCCTCCTTTGAAGAGAGTTTGGAGCATCTCTACCACCAAGTCGAGCCCCTCTA CCTGAACCTCCATGCCTTTGTCCGTCGCGCACTGCACCGCCGCTATGGGGACAAATACATCAATCTCAGA GGTCCTATTCCCGCTCATCTGCTGGGAGACATGTGGGCGCAGAGCTGGGAGAACATTTACGACATGGTAG TGCCTTTCCCGGACAAACCCAACCTCGATGTCACCAGTACAATGGTACAGAAGGGCTGGAATGCCACGCA CATGTTCCGGGTCGCAGAGGAATTCTTTACCTCGCTGGGGCTCTCCCCCATGCCTCCAGAGTTCTGGGCG GAGTCGATGCTGGAGAAACCAGCTGATGGACGGGAGGTGGTGTGCCATGCCTCTGCGTGGGACTTCTACA ACAGGAAGGACTTCAGGATTAAGCAGTGCACGCGGGTCACGATGGACCAGCTGTCCACAGTACACCACGA GATGGGCCACGTGCAGTACTATCTCCAGTACAAGGACCTGCACGTCTCTCTGCGTCGAGGTGCCAACCCT GGCTTCCACGAGGCCATCGGGGATGTACTCGCTCTCTCTGTCTCTACCCCAGCACATCTGCACAAAATTG GCCTGCTAGACCGTGTTGCCAATGACATAGAAAGTGACATCAATTACTTGCTAAAGATGGCCCTAGAGAA AATTGCCTTCTTGCCCTTTGGTTACCTGGTGGACCAGTGGCGCTGGGGGGTCTTCAGTGGACGTACCCCA CCCTCTCGCTACAACTACGACTGGTGGTATCTTCGAACCAAGTATCAGGGGATCTGCCCACCAGTTGCTC GGAATGAAACCCATTTTGACGCTGGGGCCAAGTTTCACATCCCAAGCGTGACACCATACATCAGGTACTT TGTGAGTTTCGTGCTACAGTTCCAGTTCCATCAAGCGCTGTGCAAGGAGGCAGGCCACCAGGGTCCACTA CACCAGTGTGACATCTACCAGTCCACCAAGGCAGGGGCCAAGCTCCAACAGGTGCTGCAGGCTGGCTGCT CCAGGCCCTGGCAGGAGGTGCTGAAGGACCTGGTGGGTTCAGATGCGCTGGATGCCAGTGCGCTAATGGA GTACTTCCAACCAGTAAGCCAGTGGCTGCAGGAGCAGAATCAGCGGAATGGCGAGGTCCTAGGCTGGCCG GAGTATCAGTGGCGTCCACCGTTACCAGACAACTATCCAGAGGGAATTGACCTAGAGACTGATGAAGCCA AGGCTAACAGGTTCGTGGAGGAGTATGACCGGACAGCCAAGGTGTTGTGGAACGAATACGCAGAGGCCAA CTGGCATTATAACACCAACATTACCATAGAGGGCAGCAAGATCCTGCTTCAGAAAAACAAGGAAGTGTCC AACCATACCTTGAAATATGGCACCTGGGCCAAGACATTTGACGTGAGCAACTTCCAGAACTCTACCATCA AGCGGATCATAAAGAAGGTTCAGAACGTGGACCGGGCAGTGCTGCCTCCCAACGAGTTAGAAGAGTACAA CCAGATCCTGCTAGACATGGAGACGACTTACAGTGTAGCCAATGTTTGCTACACAAATGGCACTTGTCTG TCACTGGAGCCTGATCTGACAAATATAATGGCCACGTCCCGGAAATACGAAGAATTGCTTTGGGTGTGGA AGAGCTGGCGAGACAAGGTGGGGAGAGCCATCCTTCCCTTTTTCCCAAAGTACGTGGACTTCTCCAACAA GATCGCCAAGCTCAACGGCTACTCTGATGCAGGGGATTCCTGGAGATCCTCATATGAGTCCGACGACTTG GAGCAAGACCTGGAAAAACTATACCAGGAGCTGCAGCCGCTCTACCTGAACCTGCATGCCTATGTGCGCC GCTCCCTGCACCGCCATTATGGGTCTGAGTACATCAACCTGGATGGTCCCATTCCTGCTCACCTGCTAGG GAACATGTGGGCACAGACTTGGTCCAACATCTATGACTTGGTGGCACCCTTCCCTTCCGCCCCCAGTATA GATGCCACGGAGGCCATGATAAAGCAGGGATGGACACCCAGAAGGATATTTAAGGAAGCTGACAATTTTT TTACCTCCCTGGGGCTGTTACCTGTGCCCCCTGAGTTCTGGAACAAGTCAATGTTAGAGAAGCCAACCGA TGGGAGGGAGGTGGTGTGCCATGCCTCAGCCTGGGACTTCTACAACGGCAAGGACTTCAGGATCAAGCAG TGTACCTCTGTGAACATGGAGGAATTGGTGATAGCCCACCACGAAATGGGCCACATCCAGTATTTCATGC AGTACAAAGACTTGCCTGTGACCTTTCGGGAGGGCGCCAACCCCGGTTTTCATGAGGCTATTGGAGATGT TTTGGCTCTGTCTGTGTCTACACCCAAGCATCTACACAGTCTCAACCTGCTCAGCAGTGAGGGCAGTGGC TACGAGCATGACATCAACTTTCTAATGAAGATGGCCCTTGACAAGATCGCCTTCATCCCCTTCAGCTACC TCATTGACCAGTGGCGCTGGAGGGTCTTTGACGGAAGCATCACCAAGGAGAACTACAACCAGGAGTGGTG GAGTCTCAGACTGAAGTACCAGGGTCTCTGCCCTCCAGTGCCTAGATCCCAAGGTGACTTTGACCCAGGG TCCAAGTTCCACGTTCCTGCGAATGTGCCATACATCAGGTACTTTATCAGCTTCATCATCCAGTTCCAGT TCCACGAGGCACTATGTCGCGCAGCCGGGCACACCGGCCCCCTGTACAAGTGTGATATCTACCAATCCAA GGAAGCAGGGAAGCTGCTGGCAGATGCCATGAAGTTGGGCTACAGTAAGCAGTGGCCAGAAGCCATGAAG ATAATCACAGGCCAACCTAACATGTCAGCCTCTGCCATTATGAATTACTTCAAGCCACTGACTGAATGGC TCGTCACAGAGAACAGGAGACATGGAGAGACACTGGGCTGGCCGGAGTACACCTGGACACCAAACACGGC TCGTGCAGAAGGCTCCCTCCCAGAGTCCAGTCGCGTCAACTTCCTGGGTATGTACCTGGAACCACAGCAG GCCCGTGTGGGCCAGTGGGTGCTGCTCTTCCTAGGCGTCGCCCTGCTGGTGGCCACCGTGGGTCTCGCCC ACCGACTCTACAACATCCATAACCATCACAGCCTCCGCCGGCCCCACCGTGGGCCCCAGTTTGGGTCCGA GGTGGAGCTCAGACACTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012544
Insert Size	3942 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012544.1, NP_036676.1
RefSeq Size	4142 bp
RefSeq ORF	3942 bp
Locus ID	24310
UniProt ID	P47820
Gene Summary	catalyzes the conversion of angiotensin I to angiotensin II; plays a role in regulation of blood pressure [RGD, Feb 2006]

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