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F12 (NM_001014006) Rat Untagged Clone
CAT＃: RN205503
F12 (untagged ORF) - Rat coagulation factor XII (Hageman factor) (F12), (10 ug)
CNY 6,744.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | HAF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205503 representing NM_001014006
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCTCTGTTGTTCCTGGGGTCTCTGCTGATGAGTCTGGACTTGACACTTTCGGCGCCACCGTGGA AGTCCAAGGAGTTCAAGGACGGAGCTGGCGATCCCTCTGTGGTTCTCACTGTGGACGGGAAGCTCTGCCA CTTTCCCTTTCAGTACCACCGTCGCCTGTACCACAAATGCATCCACAAAGGACAGCCAGGCTCCAGGCCC TGGTGTGCTACCACCCCCAACTTTGACGAGGACCAGCAATGGGGATACTGCTTGGAGCCCAAGAAAGTGA AAGACCATTGCAGCAAACACAGCCCCTGCCACAAAGGAGGGACGTGTGTCAACACCCCCAACGGCCCGCA CTGTCTCTGCCCTGAACACCTCACCGGGAAACATTGCCAGAGAGAGAAATGCTTTGAGTCTCAGCTCCTC AAGTTCTTCCATGAGAATGAGATATGGTTTAGAACTGGGCCAGGAGGTGTGGCCAGGTGCCAGTGCAAAG GTCCTCAGGCTGTTTGCAAGCTGCTGACCAGTCAGGTTTGCAGGGTCAATCCGTGCCTTAATGGAGGCAC CTGCCTCCTCGTGGAGGACCACCGACTGTGCCACTGCCCTGCAGGCTATGCCGGACCTTTTTGCGACTTA GACCTTAAGGCGACTTGCTACGAAGACAGGGGTCTCAGCTACCGGGGCCAGGCTAAAACTACTCTGTCGG GTGCACCATGTCAGCGGTGGGCCTCGGAGGCCACCTACCGGAACATGACTGAGACGCAAGCTCTAAGCTG GGGCCTGGGCCACCACGCATTCTGCCGGAACCCAGATAATGACACACGTCCATGGTGCTACGTCTGGAGT GGCGACAGGCTGAGCTGGGACTACTGCGACCTGGAACAGTGCCAGATGCCAACGCTCACATCTCCGGTTT CCCCTGAGAGTCACGACATGCTGAAGCCCCGGCCTCCCATATTGCAGATGCCTCAGTTCCCGTCTCTGTC CGATGCACTAGACAACTCGACCCGTAATCAGAATGTTGTGTCCAGGACCAGTACGGTGGTCTGCGGACAG AGGTTTCGCAAGCGACTGTCCTCGCTCAGGCGCGTGGTGGGCGGACTAGTGGCTCTGCCTGGATCGCATC CCTACATCGCTGCACTGTACTGGGGCGACAGCTTCTGCGCAGGCAGTCTCATCGACCCCTGCTGGGTGCT GACCGCTGCTCACTGCTTGCAGAAACGGCCAGCGCCCGAGGAACTGACAGTGGTACTTGGTCAAGATCGC CATAACCAGAGCTGCGAGAGGTGCCAGACTCTGGCTGTGCACTCCTACCGCCTTCACGAGGGCTTCTCTT CCAAAACCTACCAGCATGATTTGGCTCTGCTGCGCCTGCGGGGGAGGAAAAACAGCTGCGCGATCTTGTC GCCTCATGTCCAGCCGGTGTGTCTGCCCAGCAGCGCGGCCCCACCCTCTGAGACAGTGCTCTGCGAGGTG GCCGGCTGGGGTCATCAGTTCGAGGGGGCTGAAGAATACGCCACCTTTCTGCAGGAGGCACAGGTACCCT TCATCTCCCTGGATCGCTGCTCCAGCTCTAACGTGCACGGAGACGCCATCCTGCCTGGGATGCTTTGTGC TGGCTTCTTGGAGGGAGGCGCCGATGCCTGTCAGGGTGACTCCGGGGGTCCTCTGGTATGTGATGAAGGA GTTACAGAGCGTCAGCTCACCCTGCGAGGAGTCATCAGCTGGGGCTCCGGCTGTGGTGACCGGAACAAGC CCGGGGTCTACACTGACGTGGCCAATTACCTGGATTGGATCCAGGAGCATACTGCTTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001014006 |
| Insert Size | 1812 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001014006.1, NP_001014028.1 |
| RefSeq Size | 2128 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 306761 |
| UniProt ID | D3ZTE0 |
| Gene Summary | Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205503 | F12 (Myc-DDK-tagged ORF) - Rat coagulation factor XII (Hageman factor) (F12), (10 ug) |
CNY 7,790.00 |
|
| RR205503L3 | Lenti ORF clone of F12 (Myc-DDK-tagged ORF) - Rat coagulation factor XII (Hageman factor) (F12), (10 ug) |
CNY 9,600.00 |
|
| RR205503L4 | Lenti ORF clone of F12 (mGFP-tagged ORF) - Rat coagulation factor XII (Hageman factor) (F12), (10 ug) |
CNY 10,450.00 |
