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Axin1 (NM_024405) Rat Untagged Clone
CAT＃: RN205458
Axin1 (untagged ORF) - Rat axin 1 (Axin1), (10 ug)
CNY 10,640.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Axin; axin-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205458 representing NM_024405
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGTCCCAAAATGAATGTCCAGGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAG ATGCCCCCCGGCCCCCAGTGCCTGGAGAAGAGGGAGAACTGGTATCCACTGATTCGAGGCCTGTCAACCA CAGTTTCTGCTCTGGGAAAGGTACCAGCATTAAAAGTGAGACCTCAACAGCCACCCCAAGACGTTCAGAT CTGGATCTGGGATATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAGGTGGGCTGAGTCAC TGCATTCCTTACTGGATGACCAAGATGGGATCAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGC TGACCTGCTGGACTTCTGGTTTGCCTGCAGTGGCTTCAGAAAGCTTGAGCCTTGTGACTCAAATGAGGAA AAGAGGCTGAAGCTGGCAAGAGCCATCTACCGAAAGTACATCCTGGATAGCAATGGCATTGTGTCCAGAC AAACCAAGCCAGCCACTAAGAGCTTCATAAAGGACTGCGTCATGAAGCAGCAGATCGATCCTGCCATGTT TGACCAGGCACAGACAGAAATCCAGTCCACCATGGAGGAGAACACCTACCCTTCCTTCCTTAAGTCTGAC ATTTATTTGGAGTACACAAGGACAGGCTCAGAAAGTCCAAAGGTCTGCAGTGACCAGAGCTCAGGGTCCG GGACAGGGAAGGGCATGTCTGGATACTTGCCCACTTTGAATGAAGATGAAGAATGGAAATGTGACCAAGA TGCAGATGAGGATGATGGCCGAGACTCTGTCCCCCCCAGCAGGCTCACCCAGAAGCTGCTATTGGAGACT GCTGCCCCGAGGGCCCCCTCAAGTAGACGGTACAACGAAGGCAGAGAGCTCAGGTATGGATCTTGGAGGG AGCCTGTCAACCCCTACTATGTCAACTCTGGCTATGCCCTGGCCCCAGCCACCAGTGCCAATGACAGTGA GCAGCAGAGCCTGTCCAGTGATGCTGACACTCTATCCCTCACGGACAGTAGCGTGGATGGAATCCCCCCA TACAGGATCCGTAAGCAGCACCGAAGGGAGATGCAGGAGAGTGTCCAAGTCAATGGGCGGGTACCTCTAC CTCACATTCCTCGCACTTACCGAATGCCAAAGGAGATCCGGGTAGAGCCACAGAAATTTGCTGAAGAGCT TATTCACCGTCTAGAGGCTGTCCAGCGTACTCGAGAGGCTGAAGAAAAGTTGGAGGAACGGCTGAAGCGT GTACGCATGGAGGAAGAAGGGGAAGATGGGGAAATGCCTTCTGGCCCCATGGCAAGTCACAAGCTGCCTT CTGTCCCAGCTTGGCACCATTTCCCACCTCGCTATGTGGATATGGGCTGCTCCGGACTGCGGGATGCCCA TGAGGAGAATCCTGAGAGCATCCTGGATGAGCATGTGCAAAGGGTCATGAGGACACCTGGCTGCCAGTCA CCTGGCCCAGGCCACCGCTCTCCTGACAGCGGGCATGTGGCCAAGACTGCAGTGCTAGGGGGTACAGCAT CTGGGCATGGGAAGCATGCTCCTAAGTTAGGGTTGAAGCTGGATTCAGCTGGCCTGCACCACCATAGGCA CGTCCACCACCACGTTCACCATAATTCAGCTAGACCCAAGGAGCAAATGGAGGCTGAAGCTGCCCGCAGG GTCCAGAGCAGCTTCTCATGGGGCCCAGAAACACATGGTCATGCCAAGCCCCGAAGCTATTCAGAGAGCA CAGGCACCAACCCCAGTGCTGGGGACTTGGCCTTTGGTGGTAAAGCTAGTGCACCCTCCAAAAGAAACAC CAAGAAGGCTGAATCTGGGAAGAATGCCAGTGCTGAGGTACCCAGTACCACAGAGGATGCAGAGAAGAAC CAGAAGATCATGCAGTGGATCATTGAGGGGGAGAAGGAGATCAGTAGACACCGGAAGGCAGGCCATGGGT CTTCTGGGATGAGGAAGCAGCAGGCCCATGAAAGCTCCAGGCCCTTGTCCATTGAACGTCCTGGGGCCGT GCACCCCTGGGTCAGTGCTCAGCTTCGGAACTCTGTCCAGCCTTCTCATCTCTTCATCCAAGATCCCACA ATGCCACCCAATCCAGCCCCTAATCCTCTGACCCAGCTGGAAGAGGCCCGCAGGCGTTTGGAGGAAGAAG AAAAGAGAGCAAACAAACTGCCCTCCAAGCAGAGGACAAAATCACAAAGAAAGGCAGGTGGCGGGAGTGC ACCACCATGTGACAGTATCGTTGTGGCCTACTATTTCTGTGGTGAACCAATACCCTACCGGACCCTGGTG AGGGGCCGTGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTAACCAAGAAGGGGAGCTACAGATACTACT TTAAGAAAGTGAGTGACGAGTTTGACTGTGGTGTGGTATTTGAGGAAGTACGGGAAGATGAGGCCATCCT GCCTGTCTTTGAAGAAAAGATCATCGGCAAGGTGGAAAAGGTGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024405 |
| Insert Size | 2499 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024405.1, NP_077381.1 |
| RefSeq Size | 3460 bp |
| RefSeq ORF | 2499 bp |
| Locus ID | 79257 |
| UniProt ID | O70239 |
| Gene Summary | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (By similarity). Also facilitates the phosphorylation of APC by GSK3B (By similarity). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (By similarity). Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205458 | Axin1 (Myc-DDK-tagged ORF) - Rat axin 1 (Axin1), (10 ug) |
CNY 10,640.00 |
|
| RR205458L3 | Lenti ORF clone of Axin1 (Myc-DDK-tagged ORF) - Rat axin 1 (Axin1), (10 ug) |
CNY 12,450.00 |
|
| RR205458L4 | Lenti ORF clone of Axin1 (mGFP-tagged ORF) - Rat axin 1 (Axin1), (10 ug) |
CNY 13,680.00 |
