Casp8ap2 (NM_001107921) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205346 representing NM_001107921 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAGATGATGACAATGGTGATGGAACGGGTTTGTTTGATGTCTGTCCTGCCTCTCCTCTTAAAA ATAATGATGAAGGCTCATTGGACATATATGCTGGGTTGGACAGTACTGTTTCTGACAGTACTGCTAGGTC CTGCGTGTCGTTCAGAAACTGTCTGGATTTGTATGAAGAGATCCTGACTGAAGAAGGAACTGCAAAGGAG GCAACATACAGTGACTTGCAGATAGAATATGGGAAATGTCAGCAGCAAATGAAAGATCTGATGAAAAGAT TTAAGGAAATACAGACACAGAACTTGAACTTAAAAAATGAAAACCAGTCTCTTAAGAAGAATATCTCAGC ACTTATCAAAACTGCCAGAGTGGAGATAAACCGTAAGGATGAAGAGATAAATCATCTTCACCAAAGATTG TCTGAGTTTCCACATTTTCGAAATAACCATAAAACTGCAAGAACAAAAGATTCACGATCCAAATCTCCCC ATTTGGACAATTGTTCAAAGACTGATCATGGAGTTAAAAGTGATGTTCAGAAAGATATACATCCTAACAC TTCACAACCAAACTTGGAAAAGGAAGGGAAATCACATTCTGAAGCACAGAATTCTTTGCACTTGTCTACG GGTATTGAGAAACATTGTACAAACAATGTCTGGTCACGTTCTCCTTACCAGGTTAGTGAAGGTAACTCAA ATGAAGATAATAGAAGAGGAAGGAATGGCGTCAGACATAGCCAAGGTAGCAGAGGAACTGACAGAACACA GAAAGACTTGAATAGCAGCTGTAATGATGGCGAGCCAAGGGACAAAGAGGCTGCTTCCAGACTACACGGA AACCCTGAGAAACATGGAAGCAGTGAAGCAAGGGCTGAGAACAAAATTTCAGAGATTAAAAGTAGCACTG GTTTGGGAAATAAAAGCGAGCGCAGTGCCTCTTCTTGGGAAAAAGAAACTTCCAGAGAAAGGCCACATAC TCGAGTGGAACCTCAAAATGACAAAAATCTAGAGAGACAAAATGAAAGATTACAAAATACACACAGAAAA GAGCTTCAGTCTCAGGACAAAGCAGAAAGAAAAGGTGATGTGAAGTTTAAACCAGCAGGAGAGGAGCAGG GGCATCGGGGAAGAGTGGACCGTGCATTACCTCCTCATTCCAAGAATGACATGAAATATTATGGCTTCAA TAAGTATCATCCAGAAGAGAGAAGGGGAAGGGAAGATGGTAAACGAGACAGAGGCATGAGCAGTCATGGC TTTCAAGATAGAAGATGTTCATCTTCTCTTTCAAGCAACAGAAATAGCAAATACCCACACTCCAAGGAAG TCAGTGTTGCACACCAGTGGGAAAATACACCTTTCAAAGCAGAAAGACATAGAACTGAGGACAGGAGGAA AAGAGAACGAGAAAACAAAGAAGAAAGTAGACATGTGAAAAGTGACAAAAAATCACCTCCAGAACACTTG CAAAGGACTCATAAAGACACTAAGAAAAGTACTGCTAATGGAAAGAGACAGACGGAGCAGAAACACGGTA AAGGTCCAGTCTCTACCAGCGAGCTTTCTAAAGGGACAGACAATAGAGAGTGTACAATGAAAGTGGAGAA TGGGCCAAATGAAGCAAAGGGCAAGGACTTAAAGTTAAGCTTCATGGAGAAGCTGAACTTAACTCTTTCT CCTGCTAAAAAGCAGCCTGTTTGTCAAGATAACGAGCATCAAATAATTGGTGTTCCCAAGCCCAGTGGCG TGTGTGACTCACAGTCCTTGGAGAAGACTGAAACAGTGGCATGTCTTCCTTCTGTCAGTGAACAAGATAG AGAGGAACCCAAATCAGAGTTACCAGAGCCAAAGGAGGCTCTTTCAGCAACATCTCAACCCAGGACCAGC ATTCCAGAAAATAAAGTGAAGGAAGAAAATAGATTGTTATTTAAATCTGTTGAGAATACTGTGCCTTGTG AACTGCTTGCTTGTGGCACGGAAATTTCCTTCCCAGCACCTGTAGAAATTGAGCAAGCAAGATGTTTGGT CCAATCAATGGAAGTGGAAGAGACATGTGCTGGTGCAAGGGCAGCTGCTTCTGTGGTGATGCGTGTATTA CCAGAACATGCTTCTGAAGACTTCAGCCAAGAATTGGGCACCAAAAGACATGATGATATAAATTCGTGTG ATATTTCTGAAGGTGGGAAAAAAAAGGTGGTTCTTTCACCAAAAGCAGCAGCAGCCAGTGAGAGCCATCT GGAGCCTGTGGTTGAAGAACCTAGCATTTCACCAGTAAACTGTTCGGGAGACAATAATCCTAAACTTGAA CCTTCTCTTGAAGAGAGACCTAAGGTTGAGAATAAATCTTCTCCTTTGGAGCCTTGTTTACCTAAAGAGA TGTTTGTACCTTCACCACAGAAGACTGAGTTGATTGACCACAAAATAGATACTGGAGAATCAAACTCGGT ATATCAAGATGATGATAACTCAGTTTTGAGTATTGACTTTAATAATTTGAGACCTATTCCAGAACCCATC AGCCCTCTGAATAGTCCAGTGAGACCGGTATCCAAAGTTCTTAGCATGGAGAGCTCATGTACAATTCCAC TGTATGATAGCAGCCATAAAGATGAGTTTCCATCCAATTCAACTCTTTCTACCTTCAAGAGTCAGTCTGA TCTAAATAAGGAAAATGAAAAACCAGTTCCCAAATTGGACAAATGTTCAGAAGCAGAATCCTGTAAGAAT TTGTCTGTAGATGAATTAGAAGAAGGGGAAATTAGAAGTGATGATGAAGAATCTGTAGCACAAAAGCGTT TGGAAAAGAGTGCAAGACCCAGAACTTCTACTGAAGTGCCGCCAGGTAAAATCAGCCCAGGAAGCAGGAG GAGCACTGTGCATGTGCATAAGGATAACGGGAGGACAGTTGTAAAACTCCCTCGGGACAGAATCACATGG AGCCAAAGATCAAATGAATCTAGACCCTCAAACACCGAAAGGAAAAGTAAAACAATGAGCATCTCCAGCT TGGAAAAAATACTTCCGCTTATTCTTGCACCCTCCTCCGTATGGGAAGTTATGCATATGTTACGGTTGCT AGGAAAACATGTAAGGAAAAATTACATGAAATTCAAGATAAAATTTTCATTGACACAATTTCATAGAATT ATTGAATCGGCAATTTTGAGTTTCACATCACTAATTAGATACCTTGATTTGTCTAAGATCTGTAAATCAG TAAGTACTTTACAGAAGAGTCTTTGTGACGTTATAGAATCTAACCTTAAACAAGTGAAGAAGAATGGCAT AGTTGACCGTTTATTTGAACAACAACAAACAGATATGAAAAAAAAATTGTGGAAGTTTGTGGATGAACAA CTTGATTATTTGTTTGAAAAACTTAAAAAAATCCTACTAAAGTTTTGTGATTCTGTAAAGCATGAAAATG ACAGTAGTGAAGGAAAACTTGGAAAAAAATATAAAGAGAGAGCCCAACATTCAAATTGTCAGAAGAAGAA AATGGACAACAAAGAAATACGGAGAGAAAAAGTGCTAAAATCAGAAAATACTGTGAATTTTAAATCTTCA CTGGGGTGTGAAAAATTTGAAGAAAAACATCAAGACCAAAATAAAACCAATACTACTACAGTAAAGCATG ATGTGAAAAGAACTTTTAGCACTTGCACTGATAATAATACAAAGAACTCTGAATGTAAAGAGCAGTTTCT GGGAATGAACTGCCCGAGTACCCCCAGGCCAGGAGAAACTGAAGGCAATGTGGAGGAGGGGACACCGGCG GCACAGCACGCCTGCGTTAAGTCAGAGCGGGGCTTTGAGATCCTCACCGAGCAGCAGGCGTCCAGCCTTA CTTTTAACTTAGTGAGTGACGCACAGATGGGTGAGATATTTAAAAGTTTGCTACAAGGTTCTGATCTGTT GGACACAGGTGGCACTGAAAAAGCGGAGTGGGAATTAAAGACTCCAGAGAAACAGCTGCTAGAGAGCCTC AAGTGTGAGTCTGCACCAGCTTGTACAACAGAAGAGCTAGTTTCAGAGGCGGCTTCTCTCTGTCCAAAGG TGATCAGTGATGATAATTGGTCTTTATTATCATCTGAAAAGGGCCCATCTCTATCTTCAGGGCTTTCACT GCCAGTTCATCCTGATGTGTTAGATGAAAATTGTATGTTTGAAGTATCTTCTAACCTTGCTTTAAGTAAA GATAATGTATACAGCTCAGAAAAGAGTAAGCCCTGCATTTCTTCCATACTCTTAGAAGATCTTGCGGTCT CTTTAACAGTACCGTCACCTCTGAAGTCAGATGGCCATTTGAGTTTCTTAAAGCCTGAAGTTTTGTCAAC TTCAACTCCTGAAGAAGTTATTAGTGCACATTTTAGTGAGGATGCTTTGCTTGAGGAAGAGGATGCATCT GAACAGGACATTCACTTAGCTCTAGAGTCTGATAACTCAAGCAGTAAGTCAAGCTGTTCCTCATGGACAA GCCGGTCTGTTGCTTCAGGCTTTCAGTACCACCCTAATCTTCCCATGCATGCTGTCATTATGGAAAAGTC CAATGATCATTTCATTGTGAAAATACGGCGTGCAACACCATCTACCTCCCCTGGCCTTAAACATGGTATG GTAGCTGAGGAGTCGTTGACACCTTTGCCTAGAACTGGAAAAGAAGCTGATGGGGCAGCAGAGAAAGAAC CTACCCTGTTTCAGAGTACAGTTTTAAACCCTGTCCAAGACTTGGAAAATTCCGACAAAAGTATTGATAA GAGCAAACCAACTCATGAAGATCAGAACTCTATAGTACAAACACAGGTTCCAGATATATACGAATTTCTT AAAGATGCTTCAAATAAGGTGGTTCATTGTGATCAAGTGGTTGATGATTGCTTCAAGTTGCATCAAGTAT GGGAACCAAAAGTTTCTGAGAGCCTTCAAGAATTGCCTTCAATGGAAAAAAATGCACACTCTGTTGATGA TCATCTTCCTAATACACACATAGATCTAACAAAAGATCCAGCTACTGAGACTAAAAGCTTGGAGCTAATG GATGTAACAGTTTTAGATATCGATCACTTGGAACATTCTAAACCCAACTTAGATCAAGATGCACAGATAA CGTGTACTTCGTTACAGCATGATGCTGTAGATGCTTTTATTGATTTGACACATGATGTTTCAAATGAGAG TAAAAACGAAAGTAATGAACCTGTGTTAACTGTTGAAGGTGTGGGATGCCAGGTAATATGTATAGATGAC GATAACCACAAAGAAGGAAAAGTGGGGAAGGTAAACAGTTCCTTAGAATGTACTGTTGAGGAAACCTGTA TTGACTTGACCTCAGAGTCTGCTGGCTCCTTTGAAGTAAAGAGACACACTTTAAAGTCAGAGCCTCCATC AAAGTCGGATTGTTTAGAGTTGCCTGAGACTCTGGGGAATGGTCACAAGAAGAGAAAGAACAGTCCTGCT GTGAGTCACTCTTCTCAGAAAAAGCAAAGAAAGGACATGGACTTAAGTAGTGAAAAGACCAAGAGACTTA GTCCTAATTCTGATAGAACTGGTGATGCTCACAGAAAGCATGGCAGCAAGAAGAGGGAACCTGCAGTAAG TGACACATCCTTGTCATCAGAGGTCAACCCAGAGGTGAAGGGCTCAACAGCACCACTTGCTACTTTTCCA GGAAGCCTTTCTGCAAAAAATGTTATCAAAAAGAAAGGAGAAATTATAGTTTCATGGACAAGAAATGACG ACAGGGAAATTTTACTGGAATGTCAGAAAAGAATGCCGTCCCTGAAAACATTTACCCACTTGGCTGTCAA ACTGAATAAAAATCCAAATCAGGTTTCAGAGAGGTTCCAGCAACTGAAAAAGCTTTTTGAGAAGTCAAAA TGCAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107921
Insert Size	5889 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107921.1, NP_001101391.1
RefSeq Size	6781 bp
RefSeq ORF	5889 bp
Locus ID	313128

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