Egr1 (NM_012551) Rat Untagged Clone
CAT#: RN205200
Egr1 (untagged ORF) - Rat early growth response 1 (Egr1), (10 ug)
CNY 6,560.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Krox-24; Ngf1; Ngfi; NGFI-A; zif-268 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN205200 representing NM_012551
Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGACAACTACCCCAAACTGGAGGAGATGATGCTGCTGAGCAACGGGGCTCCCCAGTTCCTCGGTGCTG CCGGAACCCCAGAGGGCAGCGGCGGCAATAACAGCAGCAGCAGCAGCAGCAGCAGCAGCGGGGGCGGTGG TGGGGGCGGCAGCAACAGCGGCAGCAGCGCTTTCAATCCTCAAGGGGAGCCGAGCGAACAACCCTACGAG CACCTGACCACAGAGTCCTTTTCTGACATCGCTCTGAATAACGAGAAGGCGCTGGTGGAGACAAGTTATC CCAGCCAAACTACCCGGTTGCCTCCCATCACCTATACTGGCCGCTTCTCCCTGGAGCCTGCACCCAACAG TGGCAACACTTTGTGGCCTGAACCCCTTTTCAGCCTAGTCAGTGGCCTTGTGAGCATGACCAACCCTCCA ACCTCTTCATCCTCAGCGCCTTCTCCAGCTGCTTCATCGTCTTCCTCTGCCTCCCAGAGCCCACCCCTGA GCTGTGCCGTGCCGTCCAACGACAGCAGTCCCATTTACTCAGCTGCACCCACCTTTCCTACTCCCAACAC TGACATTTTTCCTGAGCCCCAAAGCCAGGCCTTTCCTGGCTCTGCAGGCACAGCCTTGCAGTACCCGCCT CCTGCCTACCCTGCCACCAAGGGTGGTTTCCAGGTTCCCATGATCCCTGACTATCTGTTTCCACAACAAC AGGGAGACCTGAGCCTGGGCACCCCAGACCAGAAGCCCTTCCAGGGTCTGGAGAACCGTACCCAGCAGCC TTCGCTCACTCCACTATCCACTATCAAAGCCTTCGCCACTCAGTCGGGCTCCCAGGACTTAAAGGCTCTT AATAACACCTACCAGTCCCAACTCATCAAACCCAGCCGCATGCGCAAGTACCCCAACCGGCCCAGCAAGA CACCCCCCCATGAACGCCCGTATGCTTGCCCTGTTGAGTCCTGCGATCGCCGCTTTTCTCGCTCGGATGA GCTTACACGCCACATCCGCATCCATACAGGCCAGAAGCCCTTCCAGTGTCGAATCTGCATGCGTAATTTC AGTCGTAGTGACCACCTTACCACCCACATCCGCACCCACACAGGCGAGAAGCCTTTTGCCTGTGACATTT GTGGGAGAAAGTTTGCCAGGAGTGATGAACGCAAGAGGCATACCAAAATCCACTTAAGACAGAAGGACAA GAAAGCAGACAAAAGTGTCGTGGCCTCCTCAGCTGCCTCTTCCCTCTCTTCCTACCCATCCCCAGTGGCT ACCTCCTACCCATCCCCCGCCACCACCTCATTTCCATCCCCAGTGCCCACCTCTTACTCCTCTCCGGGCT CCTCTACCTACCCGTCTCCTGCACACAGTGGCTTCCCATCGCCCTCGGTGGCCACCACCTATGCCTCCGT CCCACCTGCTTTCCCTGCCCAGGTCAGCACCTTCCAGTCTGCAGGGGTCAGCAACTCCTTCAGCACCTCA ACGGGTCTTTCAGACATGACAGCAACCTTTTCTCCTAGGACAATTGAAATTTGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | AscI-MluI |
ACCN | NM_012551 |
Insert Size | 1527 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012551.2, NP_036683.1 |
RefSeq Size | 1527 bp |
RefSeq ORF | 1527 bp |
Locus ID | 24330 |
UniProt ID | P08154 |
Gene Summary | activates transcription of the LH receptor gene; may be involved in synaptic plasticity during REM sleep [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR205200 | Egr1 (Myc-DDK-tagged ORF) - Rat early growth response 1 (Egr1), (10 ug) |
CNY 5,672.00 |
|
RR205200L3 | Lenti ORF clone of Egr1 (Myc-DDK-tagged ORF) - Rat early growth response 1 (Egr1), (10 ug) |
CNY 8,460.00 |
|
RR205200L4 | Lenti ORF clone of Egr1 (mGFP-tagged ORF) - Rat early growth response 1 (Egr1), (10 ug) |
CNY 9,220.00 |