Kit (NM_022264) Rat Untagged Clone

CAT#: RN205111

Kit (untagged ORF) - Rat v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (Kit), (10 ug)



  "NM_022264" in other vectors (3)

CNY 12,540.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN205111 representing NM_022264
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCTGCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGGGA
CTTCTCAGCCATCTGCGAGTCCAGGGGAGCCGTCTCCACCATCCATCCAGCCGGCCCAGTCAGAGTTAAT
AGTTGAAGCCGGGGACACCATCAGGCTGACGTGCACTGACCCCGCCTTTGTCAAATGGACTTTCGAGATC
CTCGATGTAAGGATTGAGAATAAGCAGAGCGAATGGATTCGAGAAAAAGCCGAGGCCACTCACACGGGCA
AATACACGTGCGTCAGCGGCAGCGGCCTCAGGAGCTCTATTTACGTGTTCGTTAGAGATCCTGCCGTACT
TTTCCTGGTTGGCCTTCCCTTGTTTGGCAAAGAAGACAACGACGCACTGGTCCGCTGCCCCCTGACAGAC
CCACAGGTGTCCAATTACTCCCTCATTGAGTGTGATGGGAAATCTCTCCCCACGGACCTGAAGTTCGTCC
CCAACCCCAAGGCTGGCATCACCATCAAAAACGTGAAGCGCGCCTACCACCGGCTGTGCATCCGGTGTGC
TGCCCAGCGTGAGGGCAAATGGATGCGGTCTGACAAATTCACCCTCAAAGTGAGAGCAGCCATCAAAGCT
ATCCCAGTGGTGTCTGTGCCCGAAACAAGTCATCTCCTTAAGGAAGGGGACACATTTACGGTGATATGCA
CCATAAAAGACGTGTCTACATCCGTGGACTCCATGTGGATAAAGTTGAACCCTCAGCCTCAGAGCAAAGC
CCAGGTAAAGCGCAATAGCTGGCATCAGGGCGACTTCAATTACGAACGCCAGGAGACGCTGACTATCAGC
TCAGCAAGAGTTAACGATTCCGGAGTGTTCATGTGTTATGCCAATAATACTTTTGGATCAGCAAATGTCA
CAACAACCTTGAAAGTAGTAGAAAAGGGATTCATCAACATCTTCCCTGTGAAGAACACTACGGTATTTGT
AACTGATGGGGAAAATGTAGACTTGGTTGTTGAGTTCGAGGCCTACCCTAAACCTGAACACCAGCAGTGG
ATCTACATGAACAGGACGCCTACTAACAGAGGGGAGGATTATGTCAAATCCGACAACCAAAGCAACATCA
GATATGTGAACGAACTTCGCCTGACCAGATTGAAAGGCACAGAAGGAGGCACTTACACCTTTCTGGTGTC
CAACTCTGATGTCAGTGCTTCCGTGACATTTGATGTTTATGTGAACACAAAACCAGAAATCCTGACATAT
GACAGGCTCATGAATGGCAGGCTCCAGTGTGTGGCGGCGGGATTCCCGGAGCCCACAATAGATTGGTATT
TTTGTACAGGGGCAGAGCAAAGGTGTACCGTTCCTGTCCCGCCAGTAGACGTACAGATCCAGAATGCGTC
TGTGTCACCATTTGGAAAACTGGTGGTTCAGAGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGCACG
GTGGAGTGTAAGGCCTCCAACGCTGTGGGCAAGAGCTCTGCCTTCTTTAACTTTGCATTTAAAGGTAACA
GCAAAGAGCAAATCCAGCCCCACACCCTGTTCACGCCGCTGCTCATTGGCTTCGTGGTCACAGCCGGCTT
GATGGGGATCATTGTGATGGTTCTTGCCTACAAATATTTGCAGAAACCCATGTATGAAGTACAATGGAAG
GTTGTCGAGGAGATAAATGGGAACAATTATGTTTACATAGACCCAACGCAGCTTCCTTATGACCACAAAT
GGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACCTTGGGAGCTGGTGCCTTTGGGAAGGTAGTTGA
GGCCACTGCCTATGGCTTAATTAAGTCGGATGCCGCCATGACGGTTGCCGTGAAGATGCTCAAACCAAGT
GCCCATTTAACGGAAAGGGAGGCCCTAATGTCAGAACTGAAGGTCCTGAGCTACCTGGGTAATCACATGA
ATATCGTCAACCTCCTTGGAGCGTGTACCGTGGGAGGGCCCACCCTGGTCATTACAGAATACTGTTGCTA
TGGTGATCTTTTGAATTTCTTGAGAAGAAAGCGTGACTCGTTTATTTTCTCAAAGCAAGAAGAACAGGCA
GACGCCGCACTTTATAAGAACCTTCTGCATTCAAAGGAGTCTTCCTGTGACAGCTCAAACGAGTACATGG
ACATGAAGCCTGGCGTTTCCTACGTCGTACCAACCAAGACAGACAAAAGGAGATCCGCAAGAATAGACTC
GTATATAGAAAGAGACGTGACTCCCGCCATCATGGAAGATGACGAGCTGGCTCTGGACCTGGAAGATTTG
CTGAGCTTTTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCGCCTCCAAGAACTGTATTCACAGAGATT
TGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAAGATTTGCGATTTCGGCCTAGCCAGAGA
CATCAGGAATGATTCGAATTACGTGGTAAAAGGAAATGCACGGCTGCCCGTGAAGTGGATGGCACCGGAG
AGCATTTTCAACTGCGTGTACACATTTGAAAGTGACGTCTGGTCCTATGGGATTTTCCTCTGGGAGCTAT
TCTCTCTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGATTCCAAGTTTTACAAGATGATCAAGGAAGG
TTTCCGAATGCTCAGCCCTGAGCACGCGCCTGCCGCAATGTATGAAGTTATGAAGACTTGCTGGGATGCT
GATCCCCTGAAAAGGCCAACATTCAAGCAGGTTGTTCAGCTCATTGAGAAGCAGATCTCAGACAGCAGCA
AACATATTTACTCCAACTTAGCAAACTGTAACCCCAACCCAGAGAACCCCGTGGTGGTGGACCATTCTGT
GAGGGTCAATTCCGTCGGCAGCAGCACCTCTTCCACACAGCCTCTCCTCGTGCATGAGGACGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_022264
Insert Size 2937 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022264.1, NP_071600.1
RefSeq Size 3816 bp
RefSeq ORF 2937 bp
Locus ID 64030
Gene Summary transmembrane receptor; deletion mutation is associated with anemia and deficiency in mast cells and melanocytes in the Ws mutant allele of rats [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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