Sh3glb1 (NM_001011929) Rat Untagged Clone

CAT#: RN204100

Sh3glb1 (untagged ORF) - Rat SH3-domain GRB2-like endophilin B1 (Sh3glb1), (10 ug)



  "NM_001011929" in other vectors (3)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN204100 representing NM_001011929
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACATCATGGATTTCAACGTGAAGAAGCTGGCGGCCGACGCGGGCACTTTCCTCAGCCGGGCCGTGC
AGTTCACAGAGGAAAAGCTTGGCCAAGCAGAGAAGACAGAACTGGATGCTCACCTGGAAAATCTCCTCAG
CAAGGCGGAGTGCACCAAAGTGTGGACAGAGAAGATAATGAAGCAGACTGAGGTGCTGTTGCAGCCAAAT
CCAAATGCCAGGATAGAAGAATTTGTTTATGAGAAACTGGACAGAAAAGCGCCAAGTCGTATAAACAACC
CAGAACTTTTGGGACAGTATATGATTGATGCAGGCACTGAGTTTGGCCCAGGGACAGCTTATGGTAATGC
CCTTATTAAATGTGGAGAAACACAGAAGCGAATTGGAACAGCTGACAGAGAGCTGATTCAAACATCAGCC
TTAAATTTCCTCACTCCTTTAAGAAACTTTATAGAAGGGGATTACAAGACAATCGCAAAAGAAAGGAAGC
TATTACAGAATAAGAGACTGGATTTGGATGCTGCAAAAACAAGACTAAAAAAGGCAAAAGCTGCAGAAAC
TAAAAGTTCATCTGAACAGGAATTAAGAATAACTCAAAGTGAATTTGATCGTCAGGCAGAGATTACCCGT
CTTCTGCTGGAAGGAATCAGCAGTACACACGCCCATCATCTCCGCTGTCTGAATGACTTTGTAGAAGCCC
AGATGACTTACTATGCACAATGTTACCAGTACATGTTAGACCTACAAAAACAACTGGGAAGTTTTCCATC
CAATTATGTTTCTAACAACAATCAGACTTCTGGGACACCTGTGCCGTATACTTTGTCAAATACCATTGGT
CCTTCTGCTGTGGCTTCAACAGGTAGCCTGGTAATCACCTGTCCTCCTAACCTCAGTGACCTTAAAGACT
CCAGCAGCACCAGGAAGGCCAGGGTGCTGTACGATTATGACGCTGCCAACAGTACGGAGCTGTCACTCCT
GGCAGACGAGGTAATCACTGTGTTCAGTGTCGTTGGAATGGACTCCGACTGGCTAATGGGGGAGAGAGGA
AATCAGAAGGGCAAGGTGCCAATTACCTACTTAGAGCTTCTCAATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001011929
Insert Size 1098 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001011929.1, NP_001011929.1
RefSeq Size 1795 bp
RefSeq ORF 1098 bp
Locus ID 292156
UniProt ID Q6AYE2
Gene Summary May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation. Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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