Htt (NM_024357) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hd; Hdh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203918 representing NM_024357 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACCCTGGAAAAACTGATGAAGGCTTTCGAGTCGCTCAAGTCGTTCCAGCAGCAACAGCAGCAGC AGCAGCCGCCGCCGCAGGCGCCGCCACCACCGCCGCCGCCGCCGCCTCAACCCCCTCAGCCGCCGCCTCA GGGGCAGCCGCCGCCACCACCGCCGCTGCCAGGTCCGGCCGAGGAGCCGCTGCACCGACCAAAGAAGGAA CTCTCAGCCACCAAGAAGGACCGTGTGAATCACTGTCTAACAATATGTGAAAACATTGTGGCACAGTCTC TCAGAAATTCTCCAGAATTTCAGAAACTCTTGGGCATTGCTATGGAACTGTTTCTGCTGTGCAGCGACGA TGCGGAGTCAGACGTCAGAATGGTGGCTGATGAGTGCCTCAACAAAGTCATCAAAGCTTTGATGGACTCT AATCTTCCAAGGCTACAGTTAGAACTCTATAAGGAAATTAAAAAGAATGGTGCTCCTCGAAGTTTGCGTG CAGCTCTGTGGAGGTTTGCTGAGCTGGCTCACCTGGTTCGACCTCAGAAGTGCAGGCCTTATCTGGTGAA TCTTCTTCCATGTTTGACCCGAACAAGCAAACGACCGGAGGAGTCAGTTCAGGAGACTTTGGCTGCAGCT GTTCCTAAAATTATGGCCTCTTTTGGCAATTTCGCGAATGACAATGAAATTAAGGTTCTATTGAAAGCTT TCATAGCAAATCTGAAGTCAAGCTCTCCCACTGTGCGGCGGACAGCAGCTGGGTCAGCAGTGAGTATCTG CCAGCACTCTAGGAGGACACAGTACTTCTACAACTGGCTCCTGAATGTGCTCCTAGGTTTGCTGGTTCCC ATGGAGGAAGACCACCCCACTCTCCTGATCCTTGGTGTGTTGCTCACACTGAGGTGTCTAGTGCCCTTGC TCCAGCAGCAGGTCAAGGACACAAGTCTAAAGGGCAGCTTTGGGGTAACACGGAAAGAAATGGAAGTCTC TCCTTCTGCAGAGCAGCTTGTCCAGGTTTATGAACTGACTTTGCATCACACACAGCACCAAGACCATAAT GTGGTGACAGGGGCATTGGAGCTCCTGCAGCAGCTCTTCCGTACCCCTCCACCTGAGCTGCTGCAAGCAC TGACCACACCAGGAGGGCTCGGGCAGCTCACTCTGGTTCGAGAGGAAGCCGGGGGCCGAGGCCGCAGCGG GAGTATCGTGGAGCTTTTAGCTGGAGGGGGTTCCTCATGCAGCCCTGTTCTCTCAAGAAAGCAAAAAGGC AAAGTGCTCTTAGGAGAGGAAGAAGCCTTGGAGGATGACTCGGAGTCCAGGTCAGATGTCAGCAGCTCAG CCTTTGCAGCCTCTGTGAAGAGTGAGATTGGTGGAGAGCTCGCTGCTTCTTCTTCGGGTGTCTCCACTCC CGGTTCTGTAGGTCACGACATCATCACTGAGCAGCCTCGATCCCAGCACACACTTCAAGCAGACTCTGTG GATTTGTCAGGCTGTGACTTGACCAGTGCTGCTACTGATGGAGATGAGGAAGACATCTTGAGCCACAGCT CCAGCCAGTTCAGTGCTGTTCCATCCGACCCTGCCATGGACCTGAATGATGGGACCCAGGCCTCCTCACC CATCAGTGACAGTTCTCAGACCACCACTGAAGGACCTGATTCAGCTGTGACTCCTTCTGACAGTTCTGAA ATTGTCTTAGATGGTGCTGACAGCCAGTATTTAGGCGTGCAGATAGGACAGCCACAGGAGGAAGACGAGG AGGAAGCTGCAGGTGTTCTTTCTGGTGAAGTCTCAGACGTTTTCAGAAACTCTTCTCTGGCCCTTCAGCA GGCACACTTGTTGGAAAGAATGGGTCATAGCCGGCAGCCTTCTGACAGCAGTGTTGATAAGTTTGTTTCA AAAGATGAGGTTGCTGAAGCTGGGGACCCAGAAAGCAAGCCTTGCCGAATCAAAGGTGACATAGGACAGC CTAATGATGATGATTCTGCTCCTCTGGTACATTGTGTCCGTCTTTTATCCGCTTCCTTTTTGTTAACTGG CGAAAAGAAAGCACTGGTTCCAGACAGAGATGTGAGAGTCAGTGTGAAGGCCCTGGCCCTCAGCTGTATT GGTGCAGCTGTGGCCCTTCATCCAGAGTCGTTCTTCAGCAAACTCTACAAAGTACCTCTCAGTACCATGG AAAGTACTGAGGAACAGTATGTCTCTGACATCCTGAACTACATCGATCATGGAGACCCTCAGGTGCGAGG AGCTACTGCCATTCTCTGTGGGACCCTTGTCTACTCCATCCTCAGCAGGTCCCGTCTCCGTGTTGGTGAC TGGCTGGGCACCATCAGGGCCCTGACAGGAAATACATTTTCTCTGGTGGACTGCATTCCTTTACTGCAGA AAACTTTGAAGGATGAATCTTCTGTTACTTGCAAGTTGGCTTGTACAGCTGTGAGGCACTGTGTCCTGAG TCTTTGCAGCAGCAGCTACAGTGACTTGGGATTACAACTGCTTATTGACATGCTGCCTCTGAAGAACAGC TCCTACTGGCTGGTGAGGACTGAACTGCTGGAAACTCTTGCAGAGATTGATTTCAGGCTGGTGAGTTTTT TGGAGGCAAAAGCAGAAAGTTTACACCGAGGGGCTCATCATTATACAGGGTTTCTAAAACTACAAGAACG AGTACTCAATAATGTGGTCATTTATTTGCTTGGAGATGAAGACCCCAGGGTTCGACATGTTGCTGCGACG ACATTGACAAGACTTGTCCCAAAGCTGTTTTATAAGTGTGACCAAGGACAGGCTGACCCAGTCGTGGCTG TAGCAAGAGATCAAAGTAGTGTTTACCTGAAGCTCCTCATGCATGAGACCCAGCCACCATCCCACTTCTC CGTCAGCACCATAACCAGAATCTATAGAGGCTACAGCTTACTACCAAGTGTAACAGATGTCACCATGGAA AACAACCTCTCAAGAGTCGTTGCCGCAGTTTCTCATGAACTCATTACGTCAACTACACGGGCACTCACAT TTGGGTGCTGTGAAGCCTTGTGTGTTCTTTCAGCCGCCTTTCCAGTTTGCACTTGGAGTCTAGGATGGCA CTGTGGAGTGCCCCCACTGAGTGCCTCTGATGAGTCCAGGAAGAGCTGCACTGTTGGGATGGCCTCCATG ATTCTCACCTTGCTTTCATCAGCTTGGTTCCCACTGGATCTCTCAGCCCATCAGGATGCCTTGATTTTGG CTGGAAACTTGCTAGCAGCGAGTGCCCCCAAGTCTCTGAGAAGCTCATGGGCCTCGGAAGAAGAAGGCAG CTCAGCAGCCACCAGACAGGAGGAGATCTGGCCTGCCCTGGGGGATCGGACTCTGGTGCCCATGGTGGAG CAGCTTTTCTCCCACCTGCTGAAGGTGATCAATATCTGTGCTCATGTCTTGGATGACGTGACTCCTGGAC CAGCAATCAAGGCAGCTTTGCCTTCTCTCACAAACCCCCCTTCTCTAAGTCCTATTCGACGGAAAGGGAA GGAGAAAGAGCCCGGAGAACAAACATCCACTCCGATGAGTCCCAAGAAAGGTGGAGAGGCCAGTACAGCC TCTCGACAGTCAGACACCTCAGGACCTGTCACAGCGAGTAAATCATCTTCACTTGGGAGTTTCTACCATC TCCCTTCCTACCTCAGACTGCATGATGTCCTGAAAGCCACTCACGCCAACTATAAGGTCACCTTAGATCT TCAGAACAGCACTGAAAAGTTTGGGGGGTTCCTGCGCTCTGCCTTGGACGTCCTTTCTCAGATTCTAGAG CTGGCGACACTGCAGGACATTGGAAAGTGTGTTGAAGAGGTCCTTGGATACTTGAAATCCTGCTTTAGTC GAGAACCAATGATGGCGACTGTCTGTGTTCAGCAGCTATTGAAGACTCTCTTTGGGACAAACTTAGCCTC ACAGTTTGATGGCTTATCTTCCAACCCCAGCAAGTCTCAGTGCCGAGCACAGCGCCTTGGCTCTTCCAGT GTGAGGCCCGGCTTATATCACTACTGCTTCATGGCACCATACACGCACTTCACGCAGGCTTTGGCTGATG CCAGCCTGAGGAACATGGTACAGGCGGACCAGGAGCACGATGCCTCAGGGTGGTTTGATGTACTCCAGAA AGTGTCTGCTCAGTTGAAGACGAACCTTACAAGTGTCACAAAGAACCGTGCAGATAAGAACGCTATTCAT AACCACATTAGGTTATTTGAGCCTCTTGTTATAAAAGCATTGAAGCAGTACACCACGACAACATCAGTAC AACTGCAGAAGCAGGTTTTGGATTTGCTGGCACAGCTGGTTCAGCTACGGGTCAATTACTGTCTACTGGA TTCAGATCAGGTGTTCATCGGGTTTGTGCTGAAGCAGTTTGAGTACATTGAAGTGGGCCAGTTCAGGGAA TCAGAGGCAATTATTCCAAATATATTTTTCTTCCTGGTACTATTATCTTATGAGCGCTACCATTCAAAAC AGATCATTGGAATTCCTAAAATCATCCAGCTGTGTGATGGCATCATGGCCAGTGGAAGGAAGGCTGTCAC ACATGCTATTCCTGCGCTGCAGCCCATTGTCCATGACCTCTTTGTGTTAAGAGGAACAAATAAAGCTGAT GCAGGGAAAGAGCTTGAAACCCAGAAGGAGGTGGTGGTCTCAATGCTGTTACGACTCATCCAGTACCATC AGGTGCTAGAGATGTTCATCCTCGTCCTGCAGCAGTGCCACAAAGAGAATGAGGACAAGTGGAAACGGCT CTCTCGGCAGGTCGCAGACATCATCCTGCCCATGTTAGCCAAGCAGCAGATGCATATTGACTCTCATGAA GCCCTTGGAGTATTAAATACCTTGTTTGAGATTTTGGCTCCTTCCTCCCTACGTCCTGTGGACATGCTTT TGCGGAGTATGTTCATCACTCCAAGCACAATGGCATCTGTAAGCACTGTGCAGCTGTGGATATCTGGAAT CCTAGCCATTCTGAGGGTTCTCATTTCCCAGTCAACCGAAGACATTGTTCTTTCTCGTATTCAGGAGCTC TCCTTCTCTCCATATTTAATTTCCTGTCCAGTAATTAACAGGTTAAGGGATGGAGACAGTAATCCAACAC TAGGAGAACGCAGTGAAGGGAAACAAGTAAAGAATTTGCCAGAAGATACATTCTCAAGGTTTCTCTTACA GCTGGTTGGTATTCTTCTGGAAGACATTGTTACAAAACAGCTCAAAGTGGACATGAGTGAACAGCAGCAT ACATTCTATTGCCAAGAGCTCGGCACACTGCTCATGTGTCTGATCCACATATTCAAATCTGGAATGTTCC GGAGAATCACAGCCGCTGCCACTAGACTCTTCACCAGTGATGGCTGTGAAGGCAGCTTCTATACTCTAGA TAGCCTGAATGCACGGGTGCGAGCCATGGTGCCCACACACCCAGCTCTGGTACTGCTCTGGTGTCAGATC CTACTGCTCATCAACCACACTGACCACCGATGGTGGGCCGAGGTGCAGCAGACGCCCAAGAGACACAGTC TGTCCTGCACGAAGTCACTAAACCCCCAGATATCTGCTGAAGAGGATTCTGGCTCAGCAGCTCAGCTTGG AATGTGCAATAGAGAAATAGTACGAAGAGGGGCCCTTATTCTCTTCTGTGATTATGTCTGTCAGAATCTC CATGACTCAGAACACTTAACATGGCTCATTGTGAATCACATTCAAGATCTGATCAGCTTGTCCCACGAGC CTCCAGTTCAAGACTTTATTAGTGCCATTCATCGTAATTCTGCAGCTAGTGGTCTTTTTATCCAGGCAAT TCAGTCTCGCTGTGAAAATCTTTCAACTCCAACCACTCTGAAGAAAACACTTCAGTGCTTGGAAGGCATC CATCTCAGCCAGTCTGGTGCTGTGCTCACACTGTATGTGGACAGGCTACTGGGCACCCCTTTCCGTGCGC TGGCTCGCATGGTCGACACCCTGGCCTGTCGCCGAGTAGAAATGCTTTTGGCTGCAAATTTACAGAGCAG CATGGCCCAGTTGCCAGAGGAGGAACTGAACAGAATCCAGGAACACCTCCAGAACACTGGGCTTGCACAA AGACACCAAAGGCTCTATTCACTGCTGGACAGATTCCGACTCTCTACTGTGCAGGACTCACTTAGCCCCT TGCCCCCAGTCACTTCCCACCCTCTGGATGGGGATGGGCACACATCCCTGGAAACAGTGAATCCGGACAA AGACTGGTACCTCCAGCTTGTCAGATCCCAGTGTTGGACCAGGTCAGATTCTGCACTGCTGGAAGGTGCA GAGCTGGTGAACCGTATCCCTGCTGAAGATATGAGTGACTTCATGATGAGCTCGGAGTTCAACCTAAGCC TTTTGGCTCCCTGCTTAAGCCTTGGCATGAGCGAGATTGCTAATGGCCAAAAGAGTCCCCTTTTTGAAGC GGCTCGTAGGGTGACTCTGGACCGGGTGACCAATGTGGTTCAGCAGCTGCCTGCAGTCCATCAAGTCTTC CAGCCTTTCCTGCCTACAGAACCCACAGCCTACTGGAGCAAGCTGAATGATCTCTTTGGTGATACCACAT CATACCAGTCTCTGACCACACTTGCCCGTGCCCTGGCACAGTACCTGGTGGTGCTCTCCAAAGTGCCTGC TCCTTTGCACCTTCCTCCTGAGAAGGAGGGGCACACGGTGAAGTTTGTGGTAATGACACTTGAGGCCCTG TCATGGCATTTGATCCATGAGCAGATCCCACTGAGTCTGGACCTCCAAGCCGGCCTAGACTGCTGCTGCC TGGCACTGCAGGTGCCTGGCCTCTGGGGGGTGCTGTCCTCCCCAGAGTACGTGACTCATACTTGCTCCCT TATCCACTGTGTGCGATTCATCCTGGAAGCCATTGCAGTACAACCTGGAGACCAACTTCTTGGTCCGGAA AGCAGGTCACATACTCCAAGGGCTGTCAGAAAGGAGGAAGTAGACTCAGATATACAAAACCTCAGTCACA TCACTTCGGCCTGCGAGATGGTGGCAGACATGGTGGAATCCCTGCAGTCGGTGCTGGCCCTGGGCCACAA GAGGAACAGCACCCTACCTTCATTTCTCACAGCTGTGCTGAAGAACATTGTTGTCAGTCTGGCCCGCCTC CCCCTCGTTAACAGCTATACTCGTGTGCCTCCTCTGGTATGGAAACTCGGGTGGTCACCCAAGCCTGGAG GGGATTTCGGCACAGTGTTTCCTGAGATCCCTGTAGAGTTCCTCCAGGAGAAGGAGGTCCTCAAGGAGTT CATCTACCGCATCAACACCCTAGGGTGGACCAGTCGTACTCAATTCGAAGAAACTTGGGCCACCCTCCTT GGTGTCCTGGTGACTCAGCCCTTGGTGATGGAACAGGAAGAGAGCCCACCAGAGGAAGACACCGAAAGGA CCCAGATCCACGTCCTGGCTGTACAGGCCATCACCTCTCTAGTGCTCAGCGCAATGGCTGTGCCTGTGGC TGGCAATCCAGCTGTAAGCTGCTTGGAGCAACAGCCCCGGAACAAGCCACTGAAGGCTCTCGATACCAGA TTTGGAAGAAAGTTGAGCATGATCAGAGGGATTGTAGAACAAGAAATCCAAGAGATGGTTTCCCAAAGAG AGAATACTGCCACTCATCATTCTCACCAGGCATGGGATCCTGTCCCTTCTCTGTTACCAGCTACTACAGG TGCTCTTATCAGCCATGACAAGCTGCTGCTGCAGATCAACTCAGAGCGGGAGCCAGGCAACATGAGCTAC AAGCTGGGCCAGGTGTCCATACACTCCGTGTGGCTGGGGAACAACATCACACCCCTGAGAGAGGAGGAAT GGGATGAGGAGGAGGAGGAAGAAGCGGATGCCCCTGCGCCAACATCACCACCTGTGTCTCCAGTCAATTC CAGAAAACACCGTGCTGGGGTTGATATTCACTCCTGTTCGCAGTTTCTGCTTGAATTATACAGCCGTTGG ATCCTGCCATCCAGTGCAGCCAGAAGGACCCCTGTCATCCTGATCAGTGAAGTGGTTCGATCTCTTCTTG TGGTGTCAGACTTATTCACTGAACGTACCCAGTTTGAAATGATGTATCTGACGCTGACAGAACTACGGAG AGTGCACCCTTCAGAAGATGAGATCCTCATTCAATACCTGGTGCCTGCCACCTGTAAGGCAGCTGCTGTT CTTGGAATGGACAAAACTGTGGCAGAGCCGGTCAGCCGCCTACTGGAGAGCACACTCAGGAGCACCCACC TGCCCAGCCAGATCGGAGCCCTGCATGGCATCCTCTATGTGTTGGAGTGTGACCTCTTGGATGACACTGT AAAGCAGCTCATTCCAGTTGTTAGTGACTATCTGCTGTCCAACCTCAAAGGAATAGCCCACTGCGTGAAC ATTCACAGCCAGCAGCATGTGCTGGTGATGTGTGCCACTGCATTCTACCTGATGGAAAACTACCCTCTGG ATGTGGGGCCAGAATTCTCAGCATCTGTGATACAGATGTGTGGAGTAATGCTGTCTGGAAGTGAGGAGTC CACCCCCTCCATCATTTACCACTGTGCCCTCCGGGGTCTGGAACGGCTCCTGCTGTCTGAGCAGCTCTCT CGGCTAGACACGGAGTCCTTGGTCAAGCTAAGTGTGGACAGAGTGAATGTACAAAGCCCACACAGGGCCA TGGCAGCCCTAGGCCTGATGCTTACCTGCATGTACACAGGAAAGGAAAAAGCCAGTCCAGGCAGAGCTTC TGACCCCAGCCCTGCTACCCCTGACAGCGAGTCTGTGATTGTAGCTATGGAGCGAGTGTCTGTGCTCTTT GACAGGATCCGCAAGGGATTTCCCTGTGAAGCCAGGGTCGTGGCAAGGATCCTGCCTCAGTTTCTAGATG ACTTCTTTCCACCTCAAGATGTCATGAACAAAGTCATTGGAGAGTTCCTGTCCAACCAGCAGCCATACCC ACAGTTCATGGCCACTGTAGTATACAAGGTTTTTCAGACTCTGCACAGTGCTGGGCAGTCATCCATGGTC CGGGACTGGGTTATGCTGTCTCTGTCCAACTTCACACAAAGAACTCCAGTTGCCATGGCCATGTGGAGCC TCTCCTGCTTCCTTGTCAGTGCATCTACCAGCCCATGGGTTTCTGCAATCCTTCCACACGTCATCAGCAG GATGGGCAAACTGGAGCAGGTGGATGTGAACCTTTTCTGCCTGGTTGCCACAGACTTCTACAGACACCAG ATAGAGGAGGAATTCGACCGCAGGGCTTTCCAGTCTGTGTTTGAGGTGGTGGCAGCACCAGGAAGTCCAT ACCACAGGCTGCTTGCTTGTTTGCAAAATGTTCACAAGGTCACCGCCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_024357
Insert Size	9363 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024357.3, NP_077333.2
RefSeq Size	13189 bp
RefSeq ORF	9363 bp
Locus ID	29424
Gene Summary	necessary for normal neuronal development; mutation of human homolog is associated with Huntingtons disease [RGD, Feb 2006]

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