Tnr (NM_013045) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	J1NRM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203738 representing NM_013045 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCGAAGGGGAAACAGTAGTCTTGAAGAACATGCTCATTGGTGTAAACCTGATCCTGTTGGGAT CCATGCTCAAGCCTTCTGAATGCCGGCTGGAAGTGACTACAGAAAGGGTCCAGAGACAAACTGTGGAGGA GGAAGGAGGGGCTTCCAGCTACAACACATCCAGCAAGGAACAGCCTATGGTCTTTAACCATGTGTATAAC ATCAACGTGCCACTCGAAAGCCTCTGCTCCTCGGGGCTGGAGGCCTCAGCTGAGCAGGACGTGAGTGCCG AAGATGATACTCTGGCAGAATACACAGGCCAAACCTCAGACCATGAAAGCCAGGTCACCTTCACCCACAA GATCAACCTCCCCAAAAAAGCCTGCCCATGTGCAAGCTCTGCCCAGGTACTGCAGGAACTGTTGAGCCGA ATCGAGATGCTGGAGAGGGAGGTGTCAGTGCTGCGAGACCAGTGCAACACCAACTGCTGTCAGGAAAGTG CTGCCACAGGACAACTGGACTATGTCCCTCACTGCAGCGGCCATGGCAACTTTAGCTTCGAGTCCTGTGG CTGCATCTGCAATGAAGGCTGGTTTGGCAAGAACTGCTCAGAGCCCTACTGCCCATTGGGCTGCTCCAGT CGGGGTGTATGTGTCGATGGCCAGTGCATTTGTGACAGTGAATACAGCGGAGATGACTGTTCAGAGCTCC GGTGCCCAACAGACTGCAGTTCCCGAGGGCTCTGTGTGGATGGGGAATGTGTCTGTGAAGAGCCCTACAC AGGCGAGGACTGCAGGGAGCTGCGTTGCCCTGGGGACTGTTCAGGGAAGGGGCAATGTGCCAATGGTACC TGCCTGTGCCAAGAGGGCTATGCTGGTGAGGACTGCAGTCAGCGACGGTGTCTGAATGCTTGCAGTGGGC GAGGTCACTGCCAGGAGGGGCTCTGCATCTGTGAGGAGGGCTACCAGGGCCCTGACTGCTCAGCAGTTAC CCCTCCAGAGGACTTGCGAGTGGCTGGTATCAGCGACAGGTCCATTGAGCTGGAATGGGACGGGCCGATG GCAGTGACGGAATATGTGATCTCTTACCAGCCGAGCCTGGGGGGCCTTCAGCTCCAGCAGCGGGTGCCTG GAGATTGGAGTGGTGTCACCATCACGGAGCTGGAGCCAGGTCTCACCTACAACATCAGCGTCTACGCTGT CATTAGCAACATCCTCAGCCTTCCCATCACTGCCAAGGTGGCCACTCATCTTTCTACTCCTCAAGGGCTA CAGTTCAAGACGATCACAGAGACCACCGTGGAAGTGCAGTGGGAGCCCTTCTCTTTCTCCTTCGATGGGT GGGAGATCAGCTTCACTCCAAAGAACAATGAAGGAGGGGTGATAGCTCAGCTCCCCAGCGATGTTACGTC CTTTAACCAAACGGGACTGAAACCTGGGGAGGAGTACATTGTGAATGTTGTAGCACTAAAGGAACAAGCC CGGGGCCCTCCGACCTCTGCCAGCGTCTCCACTGTCATTGACGGGCCCACACAGATCCTGGTTCGAGATG TCTCTGATACTGTGGCCTTTGTGGAATGGACCCCACCTCGAGCCAAAGTTGATTTCATTCTATTAAAATA TGGCTTGGTGGGTGGCGAAGGTGGGAAGACTACCTTCCGGCTGCAGCCTCCCCTTAGCCAGTACTCAGTG CAAGCCCTTAGACCTGGCTCCCGCTACGAGGTGTCCATCAGCGCAGTCCGGGGGACCAATGAAAGTGATG CCTCAAGCACCCAGTTTACAACAGAAATTGATGCTCCCAAGAATTTGCGAGTGGGTTCCCGGACAGCAAC TAGCCTTGACCTCGAATGGGATAACAGCGAGGCTGAAGCTCAGGAGTACAAGGTTGTGTACAGCACCCTA GCCGGGGAGCAGTACCATGAAGTGCTGGTACCCAAAGGCATTGGTCCAACTACCAAGACTACCCTCACAG ATCTGGTTCCAGGCACAGAATATGGAGTTGGAATATCTGCGGTAATGAACTCGAAACAAAGCATTCCCGC TACCATGAATGCCAGGACTGAGCTTGACAGTCCCCGAGACCTCATGGTAACAGCTTCCTCAGAGACCTCT ATCTCTCTCATCTGGACGAAGGCCAGTGGTCCTATTGATCACTATCGAATTACTTTTACTCCATCTTCTG GGATCTCCTCAGAAGTCACTGTGCCTAGGGATAGGACTTCATATACACTGACAGATCTAGAGCCTGGAGC AGAATACATCATCTCCATTACTGCTGAGAGGGGTCGGCAGCAGAGCCTGGAGTCTACTGTGGATGCCTTC ACAGGCTTCCGCCCTATCTCCCACTTGCACTTTTCTCATGTGACCTCCTCCAGTGTCAACATCACCTGGA GTGACCCATCTCCCCCAGCAGACAGACTCATTCTGAACTACAGCCCCAGGGATGAAGAGGAGGAGATGAT GGAGGTTCTCTTGGATGCCACCAAGAGGCACGCCGTCCTAATGGGTCTACAGCCAGCCACTGAATATATA GTGAATCTCGTAGCTGTCCATGGGACAGTAACCTCTGAACCCATAGTGGGTTCTATCACTACAGGAATTG ATCCTCCCAAAAACATCACAATTAGCAACGTGACTAAGGACTCCCTGACAGTTTCCTGGAGCCCTCCTGT TGCGCCTTTTGATTACTACGAGTATCCTATCGACCACCCAAGTGGACGGCTGGACAGCTCTGTCGTGCCC AACACTGTGACAGAGTTCACAATCACCAGGCTCTATCCAGCTAGTCAATATGAAATAAGCCTCAACAGTG TACGCGGGAGGGAGGAGAGTGAACGCATCTGCACCCTGGTGCACACAGCCATGGATAGCCCCATGGATCT AATTGCTACCAACATCACGCCAACAGAAGCCCTGCTCCAGTGGAAGGCACCCATGGGTGAAGTGGAAAAT TATGTCATTGTCCTCACCCACTTTGCCATGGCTGGAGAGACCATCCTGGTTGATGGGGTCAGTGAAGAAT TCCAGCTTGTAGACCTGCTTCCTAGGACCCACTACACTGTCACCATGTATGCTACCAGTGGACCTCTCGT GAGTGGCACCATCGCCACCAACTTCTCCACCCTCCTGGACCCTCCCGCCAACCTGACAGCCAGTGAAGTC ACCAGGCAAAGCGCACTGATCTCCTGGCAGCCACCCAGAGCTGCGATTGAAAACTATGTCTTGACATACA AGTCCACCGATGGAAGCCGCAAGGAGCTGATTGTGGATGCTGAGGACACCTGGATCCGACTGGAGGGCCT GTCGGAGAACACAGACTACACAGTGCTCCTGCAGGCAGCTCAGGAGGCCACAAGGAGCAGCCTCACCTCT ACTATCTTTACCACAGGGGGCCGGGTGTTCTCTCATCCTCAAGACTGTGCCCAGCACTTGATGAATGGAG ACACTCTGAGTGGAGTTTACACCATCTTCCTCAATGGGGAGTTAAGCCACAAGTTGCAAGTATACTGCGA TATGACCACAGATGGGGGCGGCTGGATTGTTTTCCAGAGACGGCAAAATGGCCAAACTGATTTTTTCCGG AAATGGGCAGATTACCGTGTTGGCTTTGGGAATCTGGAGGATGAGTTTTGGCTAGGGCTAGACAACTACC ACAGGATAACAGCCCAGGGCCGCTATGAGCTGCGTGTGGATATGCGGGATGGACAGGAGGCCGTCTTTGC CTACTATGACAAGTTCGCTGTGGAGGACAGCAGAAGCCTGTACAAGCTCCGTATAGGAGGCTACAATGGC ACTGCAGGAGACTCCCTTAGCTATCACCAGGGACGTCCTTTCTCCACTGAGGACAGAGACAATGATGTTG CAGTCACCAACTGTGCCATGTCATACAAGGGTGCTTGGTGGTATAAGAACTGCCACCGGACCAACCTCAA CGGGAAGTATGGGGAGTCCAGGCACAGTCAGGGGATCAACTGGTACCATTGGAAAGGCCATGAATTCTCC ATCCCCTTTGTAGAAATGAAGATGAGGCCCTACATCCATCGTCTCACAGCCGGGAGGAAACGGCGAGCCT TGAAATTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013045
Insert Size	4071 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013045.1, NP_037177.1
RefSeq Size	6455 bp
RefSeq ORF	4071 bp
Locus ID	25567
UniProt ID	Q05546
Gene Summary	an extracellular matrix protein; involved in regulating neurite outgrowth [RGD, Feb 2006]

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