Elp1 (NM_080899) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ikbkap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203572 representing NM_080899 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAATCTGAAGTTGCATCAGACCCTGGAATTTAGGGACATTCAGGCACCAGGGAAACCTCAGTGTT TCTGTCTGCGAGCTGAACAGGGGACAGTTCTGATTGGCTCAGAACGTGGACTGACAGAAGTAGATCCTGT GAAAAAAGAAGTGAAGACTGAAATTTCTTTGGTGGCAGAAGGTTTTCTTCCAGAGGATGGAAGTGGCTGC ATTGTGGGTATTCAGGACTTGCTGGATCAAGAATCTGTGTGTGTGGCCACTGCCTCTGGGGACGTCATAG TCTGCAATGTCAGCACACAGCAGCTGGAATGTGTTGGGAATGTGGCCAGTGGTATCTCTGTCATGAGCTG GAGTCCTGATCAAGAACTGCTGCTGCTTGCCACAGCTCAACAGACCCTGATTATGATGACAAGAGACTAT GAAGTAATCACAGAGCAGCAGATCCACCAGGATGATTTTGGAGAAGGCAAGTTTATCACCGTTGGATGGG GCAGTAAGGACACGCAGTTCCACGGCTCAGAAGGCAGGCCAATAACCTTTCCGGTGCAAATGCATGAGTC TGCCTTGTCCTGGGATGACCACAGACCACAAATTACCTGGCGTGGGGATGGGCAATTTTTTGCTGTGAGT GTTGTTTGCTCACAAACAGGGGCTCGGAAAATCCGAGTATGGAACCGAGAGTTTGCCTTGCAGTCAACCA GTGAGTCTGTGCCAGGGCTGGGGCCCTCCCTGGCTTGGAAACCCTCAGGCAGTCTGATTGCATCTACTCA GGATAAACCCAACCAGCAAGACGTTGTGTTTTTTGAGAAAAATGGACTTCTTCATGGGTATTTTACACTT CCTTTCTTGAAAGATGAAGTTAAGGTAAATGACTTGCTGTGGAATGCAGATTCCTCGGTGCTTGCTGTGT GGCTAGAAGACCTCCCGAAGGAAGGCAGTTCCACTCTGAAAAGCTATGTCCAGCTCTGGACCGTGGGGAA CTATCACTGGTATCTCAAGCAAAGCCTGCCCTTCAGCACCACGGGGAAGAACCAGATCGTGTCTCTGCTG TGGGACCCAGTGACGCCGGGCCGACTGCATGTTCTCTGTCAGGGCTGGCGGTATCTATGCTGTGACTGGC ACTGGACCACTGATCGGAGCTCTGGAAATAGTGCCGATGACCTGGCAAATGTGGCTGTCATTGATGGAAA CAAGGTGTTGGTAACAGTCTTCCAGCGGACTGTGACCCCACCTCCCATGTGTACCTACCGGCTGCTGATC CCACATCCAGTGAATCAAGTCATGTCCTCTGCTCACCTTGGAAATGACCTTGCTGTCCTTGACGCCAGTA ACCAGATTTCTGTCTACAAGTGTGATGATAAACCAGACATGGATTCCACAGTGAAACTAGGAGCTGTGGG TGGAACTGGATTTAAAGTTCCTCTTAGAACACCTCATCTGGAAAAAAGATACAGGATTCAGTTTGGGAAC AAGGAAGAGGAGGAGGATGTAAGCCCACTGCAATTTCGATTTCTCACCTGGATTGAAGGTGACGCTTTCC TGGCCATAAGCCACAGTCACTCCAGCCCACAGTCCATCATTCACCATCTGACTATGGCCGGTTCTGAGGG GGATGAGGAACAAGGACAGCTGAATGTCAGTTCATCTGTGACAGTGGATGGAGTTGTCATTGGTTTATGT TGCTGTTCTAAGACCAAGTCATCAGCAGTACAGCTGGCTGACGGCCAGGTGCTCAAGTACCTTTGGGAGT CGCCTTCTTCGGCTGTGGAGCCCTGGAAGAACTCTGAAGGGCGTCCTGTCCGATTTGCTCGTCCATGCAC CCAGATGGAAGCAGCCGCCATTGGAGGGGAGGAATGTGTCCTTGGTCTGACCGACAGGTGTCGCTTTTTC ATCAACGACACTGAGGTTGCATCAAATATCACGTCATTTGCAGTGTGTGATGACTTTCTACTGGTGACAA CCCATTCCCACACCTGCCAGTGTTTCTCTCTAAGTGGTGCCTCACTTAAAATGCTGCAGGCAGGTCTGTG TGGCAGTCAGGTGCCCAGTGGAGAGATTCTGAGGAAGGTGGAGAGGGGTTCCCGGATCGTCACAGTCGTC CCCCAGGACACAAAGCTTATACTCCAGATGCCGAGGGGGAACTTGGAGGTTGTTCATCATCGGGCCTTGG TCTTGGCTCAGATTCGGAAGTGGCTGGACAAACTTATGTTTAAAGAAGCGTTTGAATGCATGAGGAAACT GAGAATTAACCTCAATCTGATTCATGATCATAACCCCAAGGTGTTTCTTGAAAATGTGGAAACCTTCATA AAACAGATTGATTCTGTAAATCATCTAAACTTGTTTTTCACAGAACTAAAGGAAGAAGATGTCACAAAGA CCATGTACCCTCCCCCGGTTACCAAGAGTGTCCAGGTGTCCACAAATCCTGATGGAAAGAAAGTTGACCT CATCTGTGACGCTATGAGAGTGGCCATGGAGACCATCAATCCTCGCAAGTTCTGCCTGTCCATACTCACA TCTCATGTGAAGAAGACGACTCCAGAACTGGACATTGTGCTGCAGAAGGTACACGAACTTCAAGGAAAAA TTCCCTTTGTTCCTGAGAGTGTGAGTGCAGAGGAGGCTTTGAAATACCTGCTGCTCCTGGTAGATGTTAA TGAATTATTTAATCATTCTCTTGGGACCTATGACTTTGATTTGGTCCTCATGGTAGCTGAGAAGTCACAA AAGGATCCTAAGGAATATCTTCCATTTCTTAATACACTTAAGAAAATGGAAACTAATTACCAACGGTTCA CCATAGACAAGTACTTGAAACGATATGAAAAGGCCCTTGGACACCTCAGCAAGTGTGGACCTGAGTATTT CACAGAATGCTTAAATTTGATAAAAGATAAAAACTTGTATAAAGAAGCTCTGAAGTTATATCGGCCAGAC TCACCACAGTACCAGGCGGTCAGTGTTGCTTATGGCGAGCACCTGGTGCAGGAGCTCCTGTACGAGCCAG CAGGGCTTGTGTTTGCCCGCTGTGGGGCCCATGAGAAGGCTCTGGAAGCGTTTTTGGCTTGTGGCAGCTG GCAGCAAGCCCTTTGTATGGCTGCCCAGCTTCAGATGGCCAAAGACAAGGTGGCTGGCCTTGCTAGAACT CTGGCAGGAAAGCTGGTCGAGCAGAGGAAACACAGTGAGGCAGCAACAGTCCTGGAACAGTACGCCCTGG ATTATGAAGAAGCCGTGCTCTTGCTGCTAGAAGGATCTGCCTGGGAAGAAGCTCTGAGGCTGGTATACAA GTATGACAGAGTGGATATTATAGAAACCAGCGTAAAGCCTTCCATTTTAGAAGCCCAAAAAAATTACATG GACTTTCTGGACTCTCAGACAGCCACATTCATTCGCCATAAAAACCGCTTGAAGGTGGTTCGAGAGCTCA AGAGCCAACGACCACGGGTGCATGTGGATCACGAGGTGGCCCATGGCCGAGAGACGGACCTCTTCTCGGA AACCAGCAGTATTAGGAGTGGCAGCGAGATGAGTGGCAGATACTCCCACAGTAACTCCAGGATATCTGCG AGGTCATCTAAAAACCGCCGCAAGGCAGAACGGAAGAAGCATAGCCTCAAAGAAGGGAGCCCCCTGGAGG GCCTGGCACTTCTGGAGGCGCTGAGTGAGGTGGTACAGAGCATTGAGAAGCTGAAAGATGAAGTGCACGC TATTTTAAAGGTGCTCTTTCTCTTTGAGTTCGAAGAGCAAGCAAGGGAGTTACAGAGGGCCTTTGAGAGT ACTCTGCAGCTGATGGGGACGGCACTTCCAGACATCTGGACTCTCACCGGCCAACAGAGTTCATCCACAC CTGTCCTAGGTCCCAGTTCCACTGTGAATAGTATCATGGCTTCTTACCAGCAGCAGAAGACTTGTGTCCC TGTTCTCGATGCTTGGGCTTGTTTGCCACCGAAGATCGACCAGAGAAGCCAGTGGAAGCTGAGCCTGCTG GAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_080899
Insert Size	3996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_080899.1, NP_543175.1
RefSeq Size	4645 bp
RefSeq ORF	3996 bp
Locus ID	140934
UniProt ID	Q8VHU4
Gene Summary	mutation of gene is associated with autosomal recessive disorder familial dysautonomia (FD) [RGD, Feb 2006]

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