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Crbn (NM_001015003) Rat Untagged Clone
CAT＃: RN203397
Crbn (untagged ORF) - Rat cereblon (Crbn), (10 ug)
CNY 5,700.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203397 representing NM_001015003
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCGAGGGAGATCACCAGGACGCTGCGCACAACATGGGAAACCACCTGCCGCTTCTGCCTGCTG ACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAGACCAGGACAGTAAAGAAGCCAGAAAACC AAATATCATAAACTTTGATACCAGTCTGCCAACCTCACATACATACCTGGGAGCTGATATGGAAGAGTTT CACGGCAGGACTTTGCACGACGACGACAGCTGTCAGGTGATTCCAGTTCTTCCTGAGGTGATGATGATCT TGATTCCTGGGCAGACGTTACCACTGCAGCTTTCTCACCCGCAAGAAGTCAGTATGGTTCGGAATTTAAT CCAGAAGGACAGAACCTTTGCAGTTCTTGCATACAGTAATGTGCAAGAAAGGGAAGCACAGTTTGGAACT ACAGCAGAGATCTATGCGTATCGAGAGGAGCAGGAGTTTGGAATTGAAGTAGTGAAAGTGAAAGCAATCG GACGGCAGAGGTTCAAGGTCCTTGAACTTCGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAAAT TTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTTCAGTTAGAATCACTCAATAAGTGCCAGATA TTTCCTTCAAAACCTATCTCTTGGGAAGACCAGTATTCATGTAAATGGTGGCAGAAATACCAGAAGAGGA AGTTTCACTGTGCAAATCTGACATCATGGCCTCGCTGGCTGTATTCATTATATGATGCTGAAACATTAAT GGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCTCAAAGAGGACTCTCTTCCTGCAAACCCA ATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTAAGAATTCAGCTCCTTAAAA TTGGCAGTGCTATTCAACGGCTTCGCTGTGAGTTAGACATCATGAACAAATGTACTTCCCTTTGCTGTAA ACAATGTCAAGAAACAGAAATAACAACAAAGAATGAAATATTTAGCTTATCCTTATGTGGTCCAATGGCA GCATATGTGAATCCCCATGGATATGTACATGAGACACTGACTGTGTATAAAGCTTCCAACCTGAATCTGA TAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATGCATGGACCATTGCCCAGTGTAAGATTTG TGCAAGCCACATTGGATGGAAATTTACAGCCACCAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTA ACTCGCTCTGCTCTGTTACCTACAATTCCAGAGACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTT GCTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001015003 |
| Insert Size | 1338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001015003.2, NP_001015003.1 |
| RefSeq Size | 2257 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 297498 |
| UniProt ID | Q56AP7 |
| Gene Summary | Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (By similarity). May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203397 | Crbn (Myc-DDK-tagged ORF) - Rat cereblon (Crbn), (10 ug) |
CNY 5,700.00 |
|
| RR203397L3 | Lenti ORF clone of Crbn (Myc-DDK-tagged ORF) - Rat cereblon (Crbn), (10 ug) |
CNY 7,600.00 |
|
| RR203397L4 | Lenti ORF clone of Crbn (mGFP-tagged ORF) - Rat cereblon (Crbn), (10 ug) |
CNY 8,460.00 |
