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Crbn (NM_001015003) Rat Tagged ORF Clone
CAT＃: RR203397
- TrueORF®
Crbn (Myc-DDK-tagged ORF) - Rat cereblon (Crbn), (10 ug)
"NM_001015003" in other vectors (3)
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CNY 5,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203397 representing NM_001015003
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCGAGGGAGATCACCAGGACGCTGCGCACAACATGGGAAACCACCTGCCGCTTCTGCCTGCTG ACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAGACCAGGACAGTAAAGAAGCCAGAAAACC AAATATCATAAACTTTGATACCAGTCTGCCAACCTCACATACATACCTGGGAGCTGATATGGAAGAGTTT CACGGCAGGACTTTGCACGACGACGACAGCTGTCAGGTGATTCCAGTTCTTCCTGAGGTGATGATGATCT TGATTCCTGGGCAGACGTTACCACTGCAGCTTTCTCACCCGCAAGAAGTCAGTATGGTTCGGAATTTAAT CCAGAAGGACAGAACCTTTGCAGTTCTTGCATACAGTAATGTGCAAGAAAGGGAAGCACAGTTTGGAACT ACAGCAGAGATCTATGCGTATCGAGAGGAGCAGGAGTTTGGAATTGAAGTAGTGAAAGTGAAAGCAATCG GACGGCAGAGGTTCAAGGTCCTTGAACTTCGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAAAT TTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTTCAGTTAGAATCACTCAATAAGTGCCAGATA TTTCCTTCAAAACCTATCTCTTGGGAAGACCAGTATTCATGTAAATGGTGGCAGAAATACCAGAAGAGGA AGTTTCACTGTGCAAATCTGACATCATGGCCTCGCTGGCTGTATTCATTATATGATGCTGAAACATTAAT GGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCTCAAAGAGGACTCTCTTCCTGCAAACCCA ATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTAAGAATTCAGCTCCTTAAAA TTGGCAGTGCTATTCAACGGCTTCGCTGTGAGTTAGACATCATGAACAAATGTACTTCCCTTTGCTGTAA ACAATGTCAAGAAACAGAAATAACAACAAAGAATGAAATATTTAGCTTATCCTTATGTGGTCCAATGGCA GCATATGTGAATCCCCATGGATATGTACATGAGACACTGACTGTGTATAAAGCTTCCAACCTGAATCTGA TAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATGCATGGACCATTGCCCAGTGTAAGATTTG TGCAAGCCACATTGGATGGAAATTTACAGCCACCAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTA ACTCGCTCTGCTCTGTTACCTACAATTCCAGAGACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTT GCTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203397 representing NM_001015003
Red=Cloning site Green=Tags(s) MAGEGDHQDAAHNMGNHLPLLPADSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEF HGRTLHDDDSCQVIPVLPEVMMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGT TAEIYAYREEQEFGIEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQI FPSKPISWEDQYSCKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKEDSLPANP IDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMA AYVNPHGYVHETLTVYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGL TRSALLPTIPETEDEISPDKVILCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001015003 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001015003.2, NP_001015003.1 |
| RefSeq Size | 2257 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 297498 |
| UniProt ID | Q56AP7 |
| MW | 50.9 kDa |
| Gene Summary | Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (By similarity). May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203397 | Crbn (untagged ORF) - Rat cereblon (Crbn), (10 ug) |
CNY 5,700.00 |
|
| RR203397L3 | Lenti ORF clone of Crbn (Myc-DDK-tagged ORF) - Rat cereblon (Crbn), (10 ug) |
CNY 7,600.00 |
|
| RR203397L4 | Lenti ORF clone of Crbn (mGFP-tagged ORF) - Rat cereblon (Crbn), (10 ug) |
CNY 8,460.00 |
