Mccc2 (NM_001012177) Rat Untagged Clone
CAT#: RN203166
Mccc2 (untagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10 ug)
CNY 7,220.00
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN203166 representing NM_001012177
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGCGCGCTGAGGTCAGTTCTGCGGCCCTGCAGTCGGGCCTCCGTACCCCGGCAGCGCGCCTATC ACGGGGACGCGGTGGCCCGACTGGGCACCCAGCCTGACTCGGGCTCCTCCACCTACCAGGAGAACTATGA GCAGATGAAAGCTTTAGTGAATCAACTTCATGAACGAGCCCAGTATGTAAGACTGGGAGGGAGTGAGAAA GCCCGGGCGCGACACACATCAAGAGGAAAACTGTTACCCAGAGACCGAATCGACAACCTCATAGACCCAG GGTCTCCCTTTCTGGAATTCTCCCAGTTCGCAGGTTACAAGTTATATGGCGAGGAGGAAGTCCCTGCAGG TGGCATCATCACAGGCATTGGGCGAGTGTCTGGAGTAGAATGCATGATTGTGGCTAACGATGCCACCGTC AAAGGAGGCACCTACTACCCAGTGACGGTGAAAAAGCACGTTCGGGCACAAGAGATCGCACTGCAGAACA GGCTCCCATGCATCTATCTGGTTGACTCAGGAGGGGCCAACTTACCTCGGCAAGCAGACACCTTTCCAGA CCGGGACCACTTTGGCCGCATATTCTATAATCAAGCTATTATGTCTTCTAAAAATATCACACAGATAGCT GTGGTCATGGGCTCCTGTACGGCAGGAGGTGCATATGTGCCGGCCATGGCTGATGAAAACATCATTGTAC AAAGACAGGGTACTATCTTCTTGGCTGGACCACCCTTGGTGAAGGCAGCTACCGGCGAAGAGGTGTCCGC TGAGGATCTCGGAGGTGCTGACTTGCACTGCAGAAGGTCTGGGGTCACTGACCACTATGCTTTGGATGAC CACCACGCCCTTCACTTGACAAGGAAGGTTGTGAGGAGTCTCAATTATCAGAAGAAACTGGATGTTACCG TTGAACCTTCTGAAGAGCCCTTGTTCCCCGCAGATGAATTGTATGGGATAGTCGGTGCGAACCTTAAGAG GAGCTTTGATGTCCGAGAGGTCATTGCTAGAATCGTCGATGGAAGCAGATTCAATGAGTTCAAGGCCTTG TATGGAGACACGCTCGTCACAGGATTCGCTCGAATATTTGGGTACCCAGTAGGCATCATTGGAAACAATG GAGTTTTGTTTTCTGAATCTGCCAAAAAGGGCGCTCACTTCGTCCAGCTGTGCTGCCAAAGAAATATCCC CCTGCTGTTTCTCCAGAACATCACTGGATTCATGGTTGGTAAAGACTATGAAGCCGAAGGGATTGCCAAG GATGGCGCCAAGATGGTAGCCGCAGTATCCTGTGCCAAAGTGCCTAAGATAACTGTCATAATCGGGGGAT CATATGGGGCTGGAAACTACGGAATGTGTGGCAGAGCATATAGTCCAAGATTTCTCTACATGTGGCCAAA TGCTCGCATCTCAGTGATGGGAGGAGAGCAGGCAGCCACCGTGTTAGCCACGGTGGCGAGAGACCAGAGA GCCCGTGAGGGGAAGCAGTTCTCCAGTGCAGAGGAGGCAGCTTTGAAAGAGCCCATCATCAAGAGGTTCG AAGAGGAAGGGAACCCTTACTATTCCAGTGCAAGGCTGTGGGATGATGGGATTATCGACCCCGTGGACAC CAGGCTGGTTCTGGGACTCAGCATCAGTGCAGCCCTCAATGCGCCCATCCAGAGGACAGACTTTGGCATC TTCAGGATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001012177 |
Insert Size | 1692 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001012177.1, NP_001012177.1 |
RefSeq Size | 2220 bp |
RefSeq ORF | 1692 bp |
Locus ID | 361884 |
UniProt ID | Q5XIT9 |
Gene Summary | Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR203166 | Mccc2 (Myc-DDK-tagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 7,220.00 |
|
RR203166L3 | Lenti ORF clone of Mccc2 (Myc-DDK-tagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9,120.00 |
|
RR203166L4 | Lenti ORF clone of Mccc2 (mGFP-tagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9,980.00 |