Sppl2b (NM_001014200) Rat Untagged Clone

CAT#: RN203112

Sppl2b (untagged ORF) - Rat signal peptide peptidase-like 2B (Sppl2b), (10 ug)



  "NM_001014200" in other vectors (3)

CNY 7,320.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1308556
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN203112 representing NM_001014200
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGCGGCTGGCCGCATCCCTGCTGCTACTCGCGGCCCAGGTGGCCTGTGAGTTCGGTGTAC
TGCGAGTTGTGCCCCAAAGCGGTGGGACCCGGGGCAGGGACTACTGCATCCTTTACAACCCACAGTGGGC
CCACTTGCCTCATGACCTCAACAAAGTGTCTCTTCTGAAGCTTCGAGACTTGAGCACGACTCAGCTCTGT
TCCCACCTCGATGTTCCTGTAGAAGACTTCACCAACCAGATCGCACTGGTGGCTCGGGGCAACTGTACCT
TCTATGAGAAAGTGCGGCTGGCCCAGGGCAGTGGGGCACATGGGCTGCTGATAGTCAGCAAGGAGAGACT
GGTCCCTCCAAGAGGCAACAAGACACAGTATGAGGAGATAAGCATCCCTGTGGCCCTGCTAAGCCACAGG
GACCTTCAAGACATCTTCAGGCGCTTTGGCCACGAGGTAATGGTAGCCCTGTATGCTCCGAGCGAGCCAG
TGATGGACTACAACATGGTCATCATCTTCATCATGGCCGTGGGCACCGTCGCCCTTGGTGGCTACTGGGC
TGGCAGTCATGATGTGAAGAAGTACATGAAGCATAAGCGAGACGACGTCCCTGAGAAGCAGGAGGATGAG
GCGGTGGACGTGACGCCGGTCATGATCTGCGTGTTTGTTGTCATGTGCTGCTTCATGCTTGTGCTCCTCT
ACTACTTCTATGACCGCTTGGTCTACGTGATCATTGGGATCTTCTGCCTGGCCTCCTCTACCGGCCTCTA
CAGCTGCCTGGCACCCTGTGTGCGCAAGCTGCCCTTCTGCACGTGCAGGGTCCCCGACAACAACCTGCCG
TACTTCCACAAGCGTCCACAGGCCCGCATGCTGCTGCTGGCCCTCTTCTGTGTCACTGTCTCCGTGGTGT
GGGGTGTCTTCCGTAATGAGGACCAGTGGGCATGGGTCCTGCAGGACACCCTGGGCATTGCCTTCTGCCT
GTACATGCTGAGGACCATTCGCCTGCCCACCTTCAAGGCCTGCACACTGCTGCTGCTGGTGCTCTTCGTC
TACGACATCTTCTTCGTCTTCATCACCCCCTACCTGACCAAGAGCGGCAACAGCATCATGGTGGAGGTGG
CCACTGGGCCCTCGAACTCATCCACCCATGAGAAGCTGCCCATGGTGCTGAAGGTGCCCAGGCTGAACAC
CTCGCCTCTCTCCCTGTGTGACCGGCCCTTCTCCCTTCTGGGCTTTGGAGACATCTTGGTGCCAGGGCTG
CTGGTGGCCTACTGCCACAGGTTTGATATCCAGGTGCAGTCCTCCAGGATTTACTTTGTGGCCTGCACCA
TTGCCTATGGCCTGGGCCTCCTGGTGACCTTCGTGGCTCTCGTCCTCATGCGACATGGCCAGCCTGCACT
CCTCTACTTGGTCCCCTGCACACTGCTGACCAGCTGCACCGTGGCTCTGTGGCGCCGGGAGATGGGCGCC
TTCTGGACGGGCAGCGGTTTTGCGGATGCACCTCAGACCCCGTGGGCTGCACCCCAGGGACCTGTGCCTC
CGAAGGATGTGGATGCCTCTCTCTCGGAGCAGCCTCGGGGTGAAGAGCTGGCCCAGAGCCCCCTGGCCAC
TGAGGAGGCAGGAGCCACAGACCCTGCCAAGGATCCTGACAGCCCTGTGGCCGGTCCCCTCAGCCCCTCA
AATGGGGACCAAGTCCAGCCTATACCTGTGGTGACCCCAGGGACCTCGGCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001014200
Insert Size 1734 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001014200.1, NP_001014222.1
RefSeq Size 2887 bp
RefSeq ORF 1734 bp
Locus ID 362828
UniProt ID Q5PQL3
Gene Summary Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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