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Pck1 (NM_198780) Rat Untagged Clone
CAT＃: RN202791
Pck1 (untagged ORF) - Rat phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), (10 ug)
CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | GTP; PCK; Pepck; PEPCK-C; RATPEPCK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202791 representing NM_198780
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTCAGCTGCATAATGGTCTGGACTTCTCTGCCAAGGTCATCCAGGGCAGCCTCGACAGCCTGC CCCAGGAAGTGAGGAAGTTTGTGGAAGGCAATGCCCAGCTGTGCCAGCCAGAGTATATTCACATCTGCGA CGGCTCCGAGGAGGAGTACGGGCGGCTGCTGGCCCACATGCAGGAGGAGGGTGTCATCCGCAAGCTGAAG AAATATGACAACTGTTGGCTGGCTCTCACTGACCCCAGGGATGTGGCCAGGATCGAAAGCAAGACGGTCA TCATTACCCAAGAGCAGAGAGACACCGTGCCCATCCCCAAAAGTGGGCAGAGCCAGCTGGGCCGCTGGAT GTCAGAAGAGGACTTCGAGAAAGCATTCAACGCCAGGTTCCCGGGGTGCATGAAAGGCCGCACCATGTAT GTCATCCCATTCAGCATGGGGCCGCTGGGCTCACCTCTGGCCAAGATTGGTATTGAGCTGACAGACTCGC CCTATGTGGTGGCCAGCATGCGGATCATGACACGGATGGGGACCTCTGTGCTGGAGGCCCTGGGCGATGG GGAGTTTATCAAGTGCCTCCACTCCGTGGGGTGCCCTCTCCCCTTAAAAAAGCCTTTGGTCAACAACTGG GCCTGCAACCCCGAGCTGACCCTGATTGCTCACCTCCCGGACCGCAGAGAGATCATCTCCTTCGGAAGCG GATACGGTGGGAACTCACTGCTTGGGAAGAAATGCTTTGCGCTGCGGATCGCCAGCAGGCTGGCTAAGGA GGAAGGGTGGCTGGCGGAGCACATGCTGATCCTGGGCATAACTAACCCCGAAGGCAAGAAGAAATACCTG GCAGCAGCCTTCCCCAGTGCCTGTGGGAAAACCAACCTGGCCATGATGAACCCCACCCTCCCCGGGTGGA AAGTTGAATGTGTGGGTGATGACATTGCCTGGATGAAGTTTGATGCCCAAGGCAACTTAAGGGCCATCAA CCCAGAAAACGGTTTTTTTGGAGTTGCTCCGGGCACCTCAGTGAAGACAAATCCGAACGCCATTAAGACC ATCCAGAAAAACACCATCTTCACCAACGTGGCTGAGACAAGTGATGGGGGTGTTTACTGGGAAGGCATCG ATGAGCCCCTGGCCCCAGGAGTCACCATCACTTCCTGGAAGAACAAAGAGTGGAGACCACAGGATGAGGA ACCGTGCGCCCATCCCAACTCGCGATTCTGCACCCCTGCCAGCCAATGTCCCATTATTGACCCCGCCTGG GAATCTCCTGAAGGAGTGCCCATCGAAGGCATCATTTTTGGTGGCCGTAGACCTGCAGGTGTCCCCCTTG TCTACGAAGCTCTCAGCTGGCAGCATGGGGTGTTTGTAGGAGCTGCCATGAGATCAGAGGCCACCGCTGC TGCAGAGCATAAGGGCAAGGTCATCATGCACGACCCCTTCGCTATGCGGCCCTTCTTTGGCTACAACTTC GGCAAGTACCTGGCGCACTGGCTGAGCATGGCCCACCGCCCAGCAGCCAAGTTGCCCAAGATCTTCCACG TCAACTGGTTCCGGAAAGACAAAAACGGCAAGTTCCTCTGGCCCGGATTTGGTGAGAACTCCCGCGTGCT GGAGTGGATGTTCGGACGCATCGAAGGGGAAGACAGCGCCAAGCTCACTCCCATTGGCTACGTCCCTAAG GAAGACGCCCTGAACTTGAAAGGCCTGGGGGACGTCAACGTGGAGGAGCTGTTCGGAATCTCTAAGGAAT TCTGGGAGAAGGAGGTGGAGGAGATCGACAAGTATCTGGAGGACCAGGTCAACGCCGACCTCCCTTACGA AATAGAGAGGGAGCTCCGAGCCCTGAAACAGAGAATCAGCCAGATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_198780 |
| Insert Size | 1869 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198780.3, NP_942075.1 |
| RefSeq Size | 2644 bp |
| RefSeq ORF | 1869 bp |
| Locus ID | 362282 |
| UniProt ID | P07379 |
| Gene Summary | Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202791 | Pck1 (Myc-DDK-tagged ORF) - Rat phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), (10 ug) |
CNY 7,980.00 |
|
| RR202791L3 | Lenti ORF clone of Pck1 (Myc-DDK-tagged ORF) - Rat phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), (10 ug) |
CNY 9,880.00 |
|
| RR202791L4 | Lenti ORF clone of Pck1 (mGFP-tagged ORF) - Rat phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), (10 ug) |
CNY 10,740.00 |
