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Ldhb (NM_012595) Rat Untagged Clone
CAT＃: RN202177
Ldhb (untagged ORF) - Rat lactate dehydrogenase B (Ldhb), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ldh2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202177 representing NM_012595
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACCCTTAAGGAAAAGCTCATTGCGCCAGTCGCAGACGACGAGACTGCCGTCCCGAACAACAAGA TTACTGTAGTAGGCGTTGGACAAGTTGGAATGGCTTGTGCTATCAGCATTCTGGGGAAGTCTCTGGCTGA TGAGCTTGCCCTGGTGGATGTCTTGGAAGACAAGCTCAAAGGAGAAATGATGGATCTGCAGCACGGGAGC TTATTTCTCCAGACTCCGAAAATCGTGGCTGATAAAGATTACTCCGTGACAGCCAATTCTAAGATTGTGG TGGTGACCGCGGGAGTCCGCCAGCAGGAGGGGGAGAGTCGGCTCAACCTGGTGCAGAGAAACGTCAATGT ATTCAAGTTCATTATTCCTCAGATCGTCAAGTACAGCCCCGACTGCACCATCATCGTGGTTTCCAACCCA GTGGATATTCTTACCTATGTCACCTGGAAGCTGAGCGGGCTACCTAAGCACCGCGTGATTGGAAGTGGAT GCAATCTGGATTCTGCTCGGTTTCGTTACCTCATGGCCGAAAAGCTTGGTATTCATCCCAGCAGCTGCCA CGGCTGGATCCTGGGCGAGCACGGGGACTCCAGTGTGGCAGTGTGGAGCGGGGTGAATGTGGCAGGAGTC TCCCTCCAGGAACTGAACCCAGAGATGGGAACGGACAATGACAGCGAGAACTGGAAGGAGGTGCATAAGA TGGTGGTGGACAGTGCCTATGAAGTCATCAAGCTAAAAGGCTACACCAACTGGGCCATCGGCCTAAGTGT GGCTGACCTCATCGAATCCATGCTGAAAAACCTCTCTCGGATTCACCCCGTGTCTACAATGGTGAAGGGA ATGTACGGCATCGAGAACGAAGTCTTCCTCAGTCTCCCGTGCATCCTTAATGCTCGGGGACTGACCAGCG TCATCAACCAGAAGCTGAAGGACGATGAGGTCGCTCAGCTCAGGAAGAGTGCGGACACCCTGTGGGATAT CCAGAAAGACCTCAAGGACCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012595 |
| Insert Size | 1005 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012595.2, NP_036727.1 |
| RefSeq Size | 1345 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 24534 |
| UniProt ID | P42123 |
| Gene Summary | This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol. Variants 1 and 2 encode the same isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202177 | Ldhb (Myc-DDK-tagged ORF) - Rat lactate dehydrogenase B (Ldhb), (10 ug) |
CNY 3,990.00 |
|
| RR202177L3 | Lenti ORF clone of Ldhb (Myc-DDK-tagged ORF) - Rat lactate dehydrogenase B (Ldhb), (10 ug) |
CNY 6,080.00 |
|
| RR202177L4 | Lenti ORF clone of Ldhb (mGFP-tagged ORF) - Rat lactate dehydrogenase B (Ldhb), (10 ug) |
CNY 6,650.00 |
