Plcb2 (NM_053478) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202153 representing NM_053478 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTGCTCAACCCTGTCCTGTTGCCCCCTAAGGTGAAGGCATATCTGAGCCAAGGGGAGCGCTTCA TCAAGTGGGACGATGAAACCTCAATAGCCTCCCCTGTTATCCTTCGGGTGGATCCCAAGGGCTATTACTT ATACTGGACACATCAAAGTAAGGAGATGGAATTTCTGGATGTCACGAGCATCCGAGACACTCGCTTTGGG AAGTTTGCCAAGATACCCAAGAGCCAGAAACTCCGGGAGGTATTCAACATGGACTTTCCAGACAACCACT TCCTGCTGAAAACATTCACAGTGGTATCCGGCCCGGACATGGTGGGCCTCACCTTCCACAATTTTGTCTC TTACAAGGAGAACGTAGGCAAGGATTGGGCTGAGGATGTACTGGCTCTGGCCAAACACCCAATGACAGCC AATGCTTCTCGAAGCACATTCCTAGACAAGATCCTGGTGAAGCTTAAAATGCAGCTGAGCCCTGAAGGGA AGATCCCTGTGAAGAATTTTTTCCAGATGTTTCCTGCCGATCGCAAACGTGTAGAAGCTGCCCTCAGTGC CTGTCACCTTGCAAAAGGCAAGAATGATGCTATCAATCCCGAGGACTTCCCAGAATCTGTGTACAAGAGC TTCCTCATGAGCCTCTGTCCTCGGCCAGAAATTGATGAGATCTTCACTTCTTATCATGCTAAAGCCAAAC CCTACATGACCAAGGAGCACCTGACCAAATTCATCAATCAGAAGCAACGAGACCCTCGACTCAATTCCTT GCTGTTCCCACCAGCCCGGCCTGAGCAGGTGCAGGCGCTCATTGACAAGTACGAGCCCAGCGGCATCAAT GTGCAGAGGGGCCAACTGTCACCAGAGGGCATGGTCTGGTTTCTCTGTGGACCAGAGAACAGTGTCCTGG CCCATGATACTCTGCGGATCCACCAAGACATGACACAGCCTCTGAATCACTACTTCATCAACTCCTCACA CAACACTTACCTGACAGCTGGCCAGTTTTCAGGCCCTTCCTCGGCTGAGATGTACCGCCAGGTGCTGCTA TCTGGCTGCCGTTGTGTAGAATTAGATTGTTGGAAAGGAAAGCCCCCCGACGAAGAACCCATCATCACCC ACGGCTTCACTATGACCACAGATATCTTGTTCAAGGAAGCAGTCGAAGCCATTGCAGAAAGTGCCTTTAA GACCTCCCCATATCCTGTCATCCTGTCATTTGAAAACCACGTGGACTCACCCCGCCAACAGGCTAAGATG GCCGAGTACTGCCGGACCATGTTTGGAGAGACCTTGCTCACAGAGCCCCTGGAAAACTTTCCTCTGAAAC CTGGCATGCCTCTGCCTAGCCCTGAGGACCTCCGGGGCAAGATCCTCATTAAGAATAAGAAGAACCAGTT TTCTGGGCCAGCATCCCCCAACAAGAAGCCTGATGGGGTGTCTGAGGGCGGCTTCCCATCTAGTGTCCCT GTAGAAGAGGACACGGGGTGGACTGCTGAGGACCGGACTGAGGTGGAGGAGGGAGAGGAGGAGGAGGAGG TGGAAGAAGAGGAAGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCTGATGA GGGCACAGCAGGCTTGGAGGTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTACATCCAGCCCACC AAGTTTATCTCCTTCGAGTTCTCTGCACAGAAGAACAGGAGTTACCTTGTCTCTTCCTTCACGGAGCTCA AGGCCTACGAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAGATGAGCCGCGT GTACCCCAAGGGCACGCGCATGGACTCTTCCAACTACATGCCCCAGATGTTCTGGAATGCCGGATGTCAG ATGGTTGCCCTCAATTTCCAGACAATGGATCTGCCCATGCAGCAGAACATGGCACTGTTTGAGTTCAACG GGCAGAGTGGCTACCTCCTAAAGCACGAGTTCATGCGCAGGCAGGACAAGCAGTTCAACCCCTTCTCGGT GGACCGCATTGATGTGGTGGTTGCCACTACCCTTTCCATTACGGTCATCTCTGGGCAGTTCCTGTCAGAG CGCAGTGTGCGCACCTATGTGGAAGTAGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGCTATCGTA CCAAGCTGTCACCTACTGCTAACTCCATCAATCCTGTCTGGAAGGAGGAACCCTTCATTTTTGAGAAGAT CTTGGTGCCTGAGCTGGCCTCCCTCAGGATAGCTGTAATGGAAGAAGGCGGCAAATTTATTGGACATCGC ATCATCCCCATCAATGCCTTACATTCCGGATACCACCATCTGTGCCTGCGCAGTGAGAGCAACATGCCTC TCACCATGCCTGCTCTCTTCGTCTTCCTGGAGATGAAGGACTATGTACCTGACACGTGGGCAGATCTTAC TGTGGCTCTTGCCAACCCCATCAAGTACTTCAGTGCCCATGATAAGAAGTCTGTGAAGCTCAAAGAAGTG ACAGGAAGTCTGCCTGAGAAGCTCTTCTCTGGGATTCCTGTTGCCAGCCAGTCCAACGGGGCTCCAGTCT CCGCAGGCAATGGGTCAACAGCGCCTGGGACCAAGGCCAAGGAGGAAGCTACGAAGGAAGTGGCAGAGCC ACAGACCACTAGCTTGGAAGAGCTGCGGGAGCTGAAGGGCGTGGTGAAGCTGCAGCGGAGACATGAGAAG GAGCTTCGAGAGCTGGAGCGGCGTGGAGCCCGGCGCTGGGAGGAGCTGCTGCAGCGTGGCGCTGCACAGC TAGCGGAGCTCCAGGACCCAGCAGCAAGCTGCAAGCTCCGCCCGGGCAAGGGCTCCCGCAAGAAGAGGAT CGTGCCCTGTGAGGAGACCATCGTGGTGCCTAGGGAGGTCCTCGAGGGACCGGACCCCCGCGTGCAGGAT CTGAAGGACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCTGTCCTCAAGCGTA AGGAGCAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAACAGGCTGCTGAACT CAAGAGCTTCAAGGAGACCTCGGAAACTGACACCAAAGAGATGAAGAAAAAACTGGAGGCCAAAAGGCTG GAGCGGATCCAAGCCATGACCAAAGTCACCACAGACAAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTA ACAACTCCCACATCCAGGAGGTGGTGCAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGA GAAGCTGGAAGAGAAGCAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAG GCGCTGGCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCAATGCGGGCTTGCT TCAAGGCCTGCTTCCCCACGGAAGCAGAAGAGAAGCCTGAGAGGCCCTGTGAGGCCTCTGAGGAGTCGTG TCCACAGGAGCCACTTGTGAACAAGACAGACACTCAGGAGAGCCGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053478
Insert Size	3552 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053478.1, NP_445930.1
RefSeq Size	3999 bp
RefSeq ORF	3552 bp
Locus ID	85240
UniProt ID	O89040
Gene Summary	may play a role in inositol mediated signal transduction of bitter taste perception [RGD, Feb 2006]

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