Dhx9 (NM_001107184) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202048 representing NM_001107184 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGATATTAAAAATTTTCTGTATGCCTGGTGTGGCAAAAGGAAGATGACTCCAGCCTATGAAATTA GAGCAGTAGGAAACAAAAACAGACAGAAATTCATGTGTGAGGTTCGAGTAGAAGGGTTTAATTATGCTGG TATGGGCAATTCCACCAATAAGAAAGATGCACAGAGCAATGCTGCCAGAGACTTCGTTAACTATTTGGTT CGAATCAATGAAGTCAAGAGTGAAGAAGTTCCTGCTGTTGGGATTGTACCACCCCCACCCATACTTAGTG ACACATCTGATAGCACAGCAAGTACTGGAGAAGGCTTACCACCACCCATGGGAGGACCACTTCCTCCTCA TCTGGCTCTCAAAGCAGCAGAAAATAATTCTGGAGTTGAGTCTTCATCTGGCTATGGTTCTCCTGGACCC ACCTGGGACCGAGGAGCCAACTTAAAGGATTACTACTCAAGAAAGGAAGAGCAAGAGGTGCAAGCGACTC TAGAGTCAGAAGAAGTGGATTTAAATGCTGGGCTTCATGGAAACTGGACTTTGGAAAATGCTAAGGCTCG TCTGAACCAGTATTTCCAAAAAGAAAAAATCCAAGGAGAATATAAGTACACCCAAGTGGGTCCCGACCAC AACAGGAGCTTTATTGCAGAAATGACCATTTATATCAAGCAGTTGGGCAGAAGGATTTTTGCACGTGAAC ATGGATCAAATAAGAAATTAGCAGCACAGTCATGTGCCCTCTCACTAGTCCGACAACTCTATCATCTTGG AGTGATTGAAGCTTACTCTGGTCTTACAAAGAAGAAAGAAGGAGAGAGAGTGGAGCCTTACAAAGTTTTC CTTTCCCCAGATTTGGAGCTTCAGTTGCAAAATGTGGTTCAAGAGCTAGATCTGGAAATTGTTCCCCCAC CTGTTGATCCTTCTATGCCAGTTATACTCAACATTGGGAAATTGGCTCATTTTGAACCATCTCAGAGGCA AAATGCAGTGGGAGTGGTTCCTTGGTCACCCCCACAGTCCAACTGGAATCCTTGGACAAGTAGCAACATT GATGAGGGGCCCCTTGCTTATGCTTCTACAGAACAAATAAGCATGGACCTTAAGAATGAATTAGCATACC AGATGGAACAGGATCATAATTTACAGAGTGTGTTACAAGAGAGAGAGTTGCTTCCTGTGAAGAAATTTGA AGCTGAAATTCTGGAAGCCATTAGTCAAAATTCAGTTGTGATTATCCGAGGGGCTACTGGTTGTGGTAAA ACCACGCAAGTTCCACAATACATTTTGGATGACTTCATCCAGAATGACAGAGCAGCTGAGTGCAATATTG TTGTGACTCAGCCCAGAAGGATCAGTGCAGTTGCGGTGGCTGAGCGAGTTGCTTATGAGAGAGGAGAAGA ACCTGGAAAAAGCTGTGGCTACAGTGTCCGGTTTGAGTCTATACTTCCACGCCCTCATGCCAGTATAATG TTTTGTACTGTAGGTGTGCTACTAAGAAAACTAGAAGCAGGCATTCGAGGAATCAGTCATGTTATTGTAG ATGAAATTCATGAAAGAGATATTAATACTGACTTCCTCTTAGTAGTACTGCGTGACGTGGTTCTGGCTTA TCCTGAAGTTCGTATTGTTCTTATGTCTGCAACTATTGATACCACCATGTTCTGTGAATATTTCTTCAAT TGCCCCATCATTGAAGTTTATGGAAGGACTTTCCCAGTTCAAGAATATTTTCTGGAAGATTGCATTCAGA TGACCCAATTCATTCCTCCTCCAAAGGACAAGAAAAAGAAGGATAAAGAGGATGATGGTGGTGAAGATGA TGACGCAAATTGCAATCTCATATGTGGTGACGAATATGGACCAGAAACAAAGTTGAGCATGTCTCAGTTG AATGAAAAGGAAACTCCCTTTGAACTTATTGAAGCACTCCTTAAATACATTGAAACCCTTAATGTTCCTG GAGCTGTGTTGGTATTTTTACCTGGCTGGAACTTGATTTATACTATGCAGAAGCATTTAGAAAATAACTC ACATTTCGGAAGTCACCGGTATCAGATTCTACCTCTCCATTCTCAGATTCCTCGGGAGGAACAGCGCAAA GTGTTTGATCCAGTACCAGATGGAGTAACCAAGGTTATTTTGTCCACAAACATTGCTGAAACAAGCATTA CCATAAATGATGTTGTTTATGTCATTGACTCCTGCAAACAAAAAGTGAAACTCTTTACTGCTCACAACAA TATGACCAACTATGCTACTGTATGGGCATCAAAAACAAACCTTGAGCAAAGGAAAGGGCGAGCTGGCCGT GTACGACCTGGGTTCTGTTTCCACCTTTGTAGTCGGGCTCGTTTTGACAGACTGGAGACCCACATGACAC CAGAGATGTTTAGAACACCATTGCATGAAATTGCTCTGAGCATAAAACTTCTGCGCCTAGGAGGAATTGG CCAATTCCTTGCAAAGGCAATTGAACCTCCACCTTTGGATGCAGTAATTGAAGCAGAGCATACTCTTAGA GAACTTGATGCTTTAGATGCCAATGATGAGTTGACTCCTCTTGGACGAATCCTGGCTAAACTCCCCATTG AGCCTCGTTTTGGAAAAATGATGATAATGGGGTGTATTTTCTATGTGGGAGACGCTGTCTGTACCATCTC TGCTGCTACCTGCTTTCCAGAGCCCTTTATCAGTGAAGGGAAGCGCTTGGGGTACATACATCGGAATTTT GCTGGAAACAGATTTTCTGATCATGTGGCTCTTTTATCAGTATTCCAAGCCTGGGATGATGCTAGGATGA GTGGAGAAGAAGCAGAAATACGGTTTTGTGAGCAGAAGAGACTAAATATGGCTACACTAAGAATGACTTG GGAAGCCAAAGTACAGCTTAAAGAGATACTGATTAACTCTGGCTTTCCAGAAGATTGTTTATTGACACAA GTGTTTACTAACACTGGACCAGATAATAATTTGGATGTTGTTATCTCTTTGCTGGCTTTTGGTGTGTACC CCAATGTATGCTATCATAAAGAGAAGAGAAAGATTCTTACCACTGAAGGTCGTAATGCACTTATCCACAA GTCTTCTGTGAACTGTCCTTTTAGCAGCCAAGACATGAAGTACCCATCTCCTTTCTTTGTTTTTGGTGAA AAGATTCGAACCCGAGCCATCTCTGCTAAAGGCATGACCTTAGTGACCCCTCTACAATTACTTCTCTTTG CCTCCAAGAAAGTCCAGTCTGATGGTCAGATCGTGTTTATAGATGACTGGATCAGACTGCAAATATCACA TGAAGCTGCAGCATGCATCACAGCCCTCCGGGCAGCCATGGAAGCTCTGGTTGTTGAAGTATCCAAACAA CCAAACATTATCAGCCAGTTGGACCCTGTCAATGAGCATATGCTAAACACCATCCGTCAGATTTCTAGGC CCTCAGCTGCTGGTATCAACCTTATGATTGGGAGTGTAAGGTATGGCGATGGTCCGAGGCCTCCCAAGAT GGCCCGATACGATAATGGAATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107184
Insert Size	3525 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107184.1, NP_001100654.1
RefSeq Size	4205 bp
RefSeq ORF	3525 bp
Locus ID	304859

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