Uggt1 (NM_133596) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	rUGT1; Ugcgl1; Uggt; UGT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201824 representing NM_133596 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTCCTGATTGCACTGGCCTTACTGTGCCTGTTTTCCTTAGCAGAAGCCAATTCAAAAGCCATTA CCACCTCTCTCACCACTAAGTGGTTTTCTGCTCCACTGCTGCTAGAAGCCAGTGAGTTCCTAGCAGAAGA CAGTCAAGAGAAATTTTGGAGTTTTGTAGAAGCCAGTCAAAACATTGGATCATCAGATCAACATGATACC GACCGTTCCTATTATGATGCCATATTGGAAGCTGCGTTTCGGTTCCTGTCACCTCTGCAGCAGAATTTGT TGAAGTTTTGTCTCTCTCTCCGTTCCTACTCAGCCTCAATTCAAGCCTTCCAGCAGATAGCAGTCGACGA GCCTCCACCAGAAGGATGCAAGTCATTTCTCTCAGTGCATGGAAAGCAGACTTGTGATCTGGGCACTCTT GAGAGCCTTCTGCTGACTGCACCTGACAGACCTAAACCTTTATTGTTCAAAGGAGATCACAGATATCCCT CATCAAATCCTGAAAGTCCAGTGGTCATTTTTTATTCTGAGATTGGCCATGAAGAATTTTCTAATATTCA CCACCAACTTATATCAAAAAGCAATGAAGGAAAAATTAATTATGTGTTCAGACATTATATATCTAATCCC AGGAAGGAGCCGGTCCACCTTTCTGGCTATGGTGTAGAATTGGCCATTAAGAGCACGGAGTACAAGGCCA AGGATGATACTCAGGTGAAAGGGACCGAGGTAAACACCACAGTCATTGGGGAGAACGATCCTATTGATGA AGTTCAGGGGTTCCTCTTTGGAAAATTAAGAGAACTGTACCCCAGCTTGGAAGGACAGTTGAAAGAGTTC CGGAAGCATCTCGTGGAGAGCACCAATGAAATGGCCCCCTTGAAAGTCTGGCAGCTGCAAGACCTCAGTT TCCAGACTGCTGCCCGCATCTTGGCTGCTCCTGTGGAGTTAGCTCTGGTGGTGATGAAGGACATTAGTCA GAACTTTCCTACCAAAGCCAGAGCAATAACAAAAACAGCTGTGAGCGCACAGCTTAGAGCGGAAGTGGAA GAGAACCAGAAGTATTTCAAGGGAACTATAGGATTACAGCCTGGAGACTCAGCTCTCTTCATCAACGGAC TTCATATTGATTTAGACACCCAGGATATCTTCAGTCTGTTTGATACTCTGAGAAATGAAGCCCGGGTAAT GGAGGGTCTACATAGACTAGGAATAGAAGGCCTTTCTCTACATAATATTTTGAAGCTCAACATCCAGCCG TCTGAGACTGACTACGCAGTAGACATCAGGAGTCCTGCTATTTCCTGGGTCAACAACCTAGAGGTTGATA GCCGATATAACTCATGGCCTTCAAGTTTACAAGAGTTACTTCGCCCCACGTTTCCTGGCGTTATACGGCA GATCAGAAAGAACCTACATAACATGGTTTTCATTGTTGATCCTGTTCATGAGACCACGGCAGAGCTGGTT AGCATAGCCGAGATGTTCCTCAGCAATCATATACCACTAAGGATTGGTTTTATCTTTGTGGTCAATGATT CTGAAGATGTTGATGGGATGCAAGATGCTGGAGTCGCTGTTCTGAGAGCATATAATTATGTGGGTCAGGA AGTGGATGGCTACCATGCCTTCCAGACTCTCACCCAGATCTACAACAAAGTGAGGACTGGAGAAAAGGTG AAAGTTGAGCATGTGGTCAGTGTCTTGGAGAAGAAGTACCCGTATGTTGAAGTGAATAGCATTCTGGGGA TTGATTCTGCTTATGATCAGAATCGGAAGGAAGCCAGAGGCTACTATGAGCAGACTGGTGTAGGCCCCTT GCCTGTTGTCTTGTTCAATGGGATGCCCTTTGAAAAGGAGCAGTTAGACCCCGACGAGCTGGAAACCATC ACAATGCACAAGATCTTGGAGACGACCACCTTCTTCCAAAGAGCCGTGTATTTGGGTGAACTGTCACATG ATCAAGACGTGGTAGAGTACATCATGAATCAGCCGAATGTTGTTCCAAGAATCAACTCTAGGATTTTGAC AGCTAAGCGAGAGTATCTGGATCTAACAGCAAGCAATAATTTTTATGTGGATGACTTTGCCAGATTTTCT GCCTTGGACTCTCGGGGCAAGACTGCTGCTATTGCCAACAGTATGAACTATCTGACAAAAAAAGGAATGT CCTCCAAGGAAATCTATGATGATTCCTTTATTAGGCCAGTGACTTTTTGGATTGTTGGAGATTTTGATAG CCCTTCTGGGCGGCAGTTATTATATGACGCCATTAAACATCAGAAAACCAGTAACAATGTTAGGATAAGT ATGATCAATAACCCCAGCCGAGAGATAAGTGACTCAAGCACCCCCGTCTCCAGAGCCATCTGGGCAGCTC TCCAGACACAGACCTCCAACTCTGCTAAGAACTTCATCACCAAGATGGTCAAAGAGGAGACGGCAGAGGC CCTGGCCGCAGGAGTGGACATTGGGGAATTCTCTGTCGGGGGCATGGATGTCAGTCTTTTTAAAGAGGTC TTTGAGTCTTCCAGAATGGATTTCATTTTGTCTCATGCCCTGTACTGCAGGGATGTTCTGAAACTGAAGA AGGGACAGAGAGTGGTGATCAGCAACGGAAGGATCATTGGGCCACTGGAGGACAGTGAGCTCTTCAACCA AGATGATTTCCACCTCCTGGAAAATATCATTCTGAAAACATCGGGACAGAAAATCAAGTCTCATATCCAA CAGCTTCGCGTAGAAGAAGATGTGGCCAGTGATTTGGTAATGAAGGTGGATGCTCTCCTGTCAGCGCAAC CCAAAGGAGAGGCGAGGATCGAGTACCAGTTCTTTGAAGATAAGCACAGTGCAATTAAACTGAAGCCCAA AGAAGGGGAGACATACTATGATGTGGTAGCTGTTGTCGACCCTGTCACAAGAGAAGCACAGAGGCTCGCC CCCTTGCTCTTGGTTTTGGCTCAGCTGATAAACATGAGTCTGAGAGTATTCATGAATTGCCAATCCAAGC TTTCCGACATGCCTTTAAAAAGCTTTTACCGTTATGTCTTAGAGCCGGAGATTTCTTTCACTGCAGACAA CAGCTTTGCCAAGGGACCAATAGCAAAGTTTCTGGATATGCCTCAGTCTCCGCTGTTTACTTTGAATTTG AACACACCCGAGAGTTGGATGGTAGAATCTGTCAGAACACCCTATGATCTTGATAATATTTACCTAGAAG AGGTGGACAGTATAGTGGCTGCTGAGTATGAGCTGGAGTATCTGTTACTGGAAGGTCATTGTTACGACAT CACCACAGGCCAGCCCCCTCGAGGACTGCAGTTCACGTTAGGAACTTCAGCCAACCCAACAACTGTGGAC ACAATCGTGATGGCCAATCTGGGATATTTTCAGCTCAAAGCCAACCCAGGAGCCTGGATTCTGAGACTGA GGAAGGGGCGCTCGGATGACATTTATAGGATCTACAGCCATGACGGAACAGATTCCCCTCCTGATGCAAA TGACGTTGTTGTCATCCTCAATAACTTCAAGAGCAAGATCATCAAAGTGAAGGTTCAGAAGAAGGCCGAC ATGGCTAATGAAGACTTGCTGAGCGACGGGACGAATGAGAATGAGTCTGGATTCTGGGACTCATTCAAGT GGGGCTTCTCAGGACAGAAGACTGAGGAAGTAAAGCAAGATAAGGACGACATAATCAATATTTTCTCTGT TGCATCTGGTCATCTCTACGAAAGGTTTCTTCGCATCATGATGCTATCAGTCCTGAAGAATACCAAAACT CCTGTGAAATTCTGGTTCTTGAAGAATTATTTGTCCCCCACATTTAAGGAGTTTATACCTTACATGGCCA AAAAATACAATTTCCAGTATGAGCTTGTTCAGTACAAATGGCCACGGTGGCTTCACCAGCAGACCGAGAA GCAGCGAATTATCTGGGGCTACAAGATCCTCTTCCTGGATGTGCTTTTCCCGTTGGTTGTTGACAAATTC CTCTTTGTGGATGCTGATCAGATTGTGCGGACAGATCTGAAGGAGTTAAGAGATTTCAATTTGGATGGTG CACCTTACGGTTACACGCCCTTCTGCGACAGCAGGAGAGAGATGGATGGCTACCGCTTCTGGAAGTCAGG CTACTGGGCCAGTCATTTGGCTGGACGAAAGTATCACATCAGTGCGCTGTATGTCGTGGATCTGAAGAAG TTTAGGAAAATAGCTGCTGGTGACAGACTCAGAGGACAGTACCAAGGTCTGAGTCAGGATCCCAACAGTC TTTCAAATCTTGATCAAGATTTGCCCAATAACATGATCCATCAGGTGCCAATCAAATCGCTCCCTCAGGA GTGGCTTTGGTGTGAGACGTGGTGTGATGATGCCTCTAAGAAGCGGGCCAAGACCATCGACCTGTGTAAT AATCCCATGACTAAGGAGCCCAAACTGGAGGCTGCTGTGCGGATCGTCCCTGAGTGGCAAGACTACGACC AGGAGATCAAGCAGTTGCAGACCCTCTTCCAAGAGGAGAAAGAGTTGGGGACCCTGCATGAAGAGGAGAC ACAGGAAGGATCTCAGAAGCATGAAGAATTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133596
Insert Size	4584 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133596.1, NP_598280.1
RefSeq Size	5053 bp
RefSeq ORF	4584 bp
Locus ID	171129
UniProt ID	Q9JLA3
Gene Summary	enzyme that catalyzes the addition of a glucose residue from UDP-glucose to an N -linked Man(9)GlcNAc(2)oligosaccharide [RGD, Feb 2006]

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