Rad50 (NM_022246) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201717 representing NM_022246 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGGATCGAAAAGATGAGCACTCTGGGCGTGCGAAGTTTTGGGATAGAGGATAAAGATAAGCAAA TTATCTCTTTCTTCAGCCCCCTCACAATTTTGGTTGGACCCAATGGGGCGGGGAAGACGACCATCATTGA ATGTCTAAAGTATATTTGTACTGGAGATTTCCCTCCTGGAACCAAAGGAAATACATTTGTTCATGATCCC AAGGTTGCTCAAGAAACAGATGTGCGTGCCCAAATTCGCCTGCAGTTTCGTGATGTCAATGGAGAGATGG TACTTGTGCAGAGGTCCATGCTTTGCAGTCAGAAAAGTAAAAAAACCGAATTTAAAACCCTGGAAGGAGT CATTACTAGAATAAAGCACGGTGAAAAAGTCAGTCTCAGCTCCAAATGTGCAGAAATCGACCGAGAAATG ATAAGTTGTCTTGGGGTTTCCAAGTCTGTGCTAAACAATGTTATTTTCTGCCACCAAGAAGACTCAAATT GGCCTCTAAGTGAAGGAAAGGCTCTGAAGCAGAAATTTGATGAGATTTTTTCAGCAACAAGGTACATTAA AGCCCTAGATACGCTTCGACAGGTACGACAGACACAAGGTCAGAAAGTAAAAGAATGTCAAACAGAATTG AAATATCTGAGGCAAAATAAGGAGAAAGCTTGTGAGATCCGAGATCAGATCACTAGTAAGGAAGCCCAGT TAGCATCTTCACGGGAAATTGTCAAAGCCTATGAGAATGAGCTTGAGCCATTGAAGAATCGCCTGAAAGA GATTGAACATAACCTCTCTAAAATAATGAGACTTGACAATGAAATTAAAGCCTTGGATAGCAGAAAGAAG CAAATGGAAAAAGATAACAGTGAATTAGAACAGAAGATGGAAAAGGTTTTTCAAGGGACTGATGAGCAGC TAAATGACTTGTATCACAATCACCAGAGAACTGTAAGGGAGAAAGAAAGGCGCTTGGTAGACTGTCAGCG TGAACTGGAGAAGCTGAGTAAAGAAGCTCGGCTCCTCAACCAGGAAAGAGCAGAGCTGCTTGTGGAGCAG GGTCGTCTACAACTACAGGCAGATCGACATCAAGAGCATATCCGAGCCAGAGACTCACTGATTCAGTCTT TGGCAGCACATCTTGAATTGGATGGTTTTGAGCGTGGACCATTCAGTGAAAGACAGATTAAAAACTTTCA TGAACTTGTGAGAGAGAGACAGGAGAGAGAAGCTAAAACTGCCAGCCAGCTCTTGAGCGACCTTACAGAC AAAGAAGCGCTGAAGCAGAGACAGATGGATGAGATGAGGGACAAGAAGAGCGGGCTGGGGAGGATGATTG AGCTGAAGACCGAGATCCTGACGAAGAAGCAGACTGAGCTGAGGAACGTGAGGAATGAGCTGCAGCAGCT GGAGGGCTCCTCGGACAGGATTCTGGAGCTGGACCAGGAGCTCACAAAAGCGGAACGTGAACTAAGCAAG GCTGAGAAAAATAGCAGCATAGAAACCCTAAAAGCAGAAATACTAAACCTCCAAAGTGAGAAAGCGGACC TGGACAGGAACCTGCGGAAACTGGATCAGGAGATGGAGCAGTTAAACCATCATACAACAACCCGCACACA GATGGAGATGCTTACCAAAGACAAAACTGACAAAGATGAACAGATCAGAAAAATAAAGTCCAGGCACAGT GATGAACTAACTTCACTGTTGGGATATTTTCCTAACAAAAAACAGCTTGAAGACTGGCTTCATTCTAAAT CCAAAGAGATTAATCAGACCAGGGACAGACTTGCCAAACTGAACAAAGAACTAGCTTCAGCCGAACAAAA TAAAAATCATATAAATAATGAGCTAAAGAAAAAGGAAGAGCAGCTGTCTAGTTATGAAGATAAACTGTTT GATGTTTGTGGTAGCCAAGATTTTGAAAGTGACTTAGACAGACTTAAAGAAGATATTGAAAAATCCTCAA AGCAGCGAGCCATGCTGGCTGGAGCCACAGCAGTTTACTCCCAGTTCATCACTCAGCTGACAGATGAAAA CCAGTCCTGTTGCCCAGGCTGTCAGAGAGTATTTCAGACGGAAGCTGAATTACAGGAAGTCATCAGTGAC TTGCAGTCCAAGCTGAGGCTTGCTCCAGATAAACTCAAGTCAACAGAATCAGAACTAAAAAAAAAAGAGC GGCGCCGTGATGAAATGCTGGGGCTTGTGCCCATGAGGCAAAGCATAATTGATTTGAAGGAAAAGGAAAT ACCAGAATTAAGAAACAGACTGCAGAGTGTCAATAGAGACATACAGCGCCTAAAGAATGACATAGAGGAG CAGGAGACACTCTTGGGTACAGTGATGCCCGAAGAGGAAAGTGCTAAAGTGTGCCTGACAGACGTCACGA TCATGGAGAGGTTCCAGATGGAGCTAAAAGACGTTGAAAGGAAAATTGCACAGCAGGCAGCTAAGCTGCA GGGGGTAGACTTGGATCGGACTGTCCAGCAGGTTAACCAGGAAAAACAAGAAAAACAACACAAACTGGAT ACAGTTTCCAGTAAGATTGAATTGAACCGTAAGCTTATACAGGACCAGCAGGAACAAATTCAGCACCTGA AAAGTAAAACAAATGAGCTGAAATCAGAGAAACTGCAGATAGCCACCAATTTGCAACGGCGTCAGCAAAT GGAGGAGCAGACTGTGGAATTATCCACTGAAGTTCAGTCTTTGAACAGAGAAATAAAGGATGCTAAAGAG CAAATAAACCCTTTGGAGATAGCACTGGAAAAGTTGCAGCAGGAAAAAGAAGAATTGATCCACAGAAAAA ATACAAGTAACAAAATGGCTCAGGATAAGATCAATGATATCAAAGAGAAAGTTAAAAATATTCATGGTTA CATGAAAGACATAGAGAATTATATTCAAGATGGAAAAGATGACTATAAGAAGCAAAAAGAAACTGAACTT AATGAAGTTGTCATTCAACTAAATGAATGTGACAAACACAAAGAAAAGATAAATAAAGAAATGGGAACCA TGAGGCAAGATATTGACACGAAGAAGATACAGGAAAGGTGGTTACAGGATAACCTTACACTGAGAAAAAG AAGAGAGGAACTAAAAGAAGTTGAAGAGGAACGAAAGCAACATTTGAAGGAGATGGGACAGATGCAAGTT TTACAGATGAAAAATGAGCACCAGAAGTTGGAAGAGAACATAGACACGATAAAGAGAAATCACAGTTTGG CATTGGGGCGACAGAAAGGCTATGAAGAAGAAATTCTTCACTTTAAGAAGGAGCTCCGAGAACCTCAGTT TCGGGATGCAGAGGAGAAGTACAGAGAAATGATGATTGTCATGAGAACCACAGAGCTGGTGAATAAGGAC CTGGACATCTACTACAAAACTCTTGATCACGCAATAATGAAATTTCACAGTATGAAAATGGAAGAAATCA ACAAAATTATTCGTGATCTTTGGCGGAGTACCTATCGTGGGCAAGATATTGAATACATAGAAATTCGATC CGATGCTGATGAAAATGTATCAGCTTCTGATAAAAGGCGAAATTACAACTACCGAGTGGTGATGCTGAAG GGGGATACAGCCTTGGACATGCGCGGACGATGCAGTGCTGGACAGAAGGTGCTGGCCTCTCTCATCATCC GACTGGCCCTGGCCGAAACCTTCTGTCTGAACTGCGGCATCCTTGCCTTGGATGAGCCTACAACAAACCT GGACCGAGAAAACATCGAGTCTCTGGCACATGCTTTGGTTGAGATTATAAAAAGTCGCTCGCAGCAGCGC AACTTCCAGCTTCTGGTCATCACTCACGATGAAGATTTTGTGGAGCTCCTAGGACGATCTGAGTATGTGG AGAAATTCTACAGAGTGAAGAAGAACATCGACCAGTGCTCAGAGATTGTCAAGAGCAGCATCAACTCTCT GGGCTCTTATGTTCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022246
Insert Size	3939 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022246.1, NP_071582.1
RefSeq Size	4444 bp
RefSeq ORF	3939 bp
Locus ID	64012
UniProt ID	Q9JIL8
Gene Summary	involved in DNA double-strand break repair [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.