Rad50 (NM_022246) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201717 representing NM_022246 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGGATCGAAAAGATGAGCACTCTGGGCGTGCGAAGTTTTGGGATAGAGGATAAAGATAAGCAAA TTATCTCTTTCTTCAGCCCCCTCACAATTTTGGTTGGACCCAATGGGGCGGGGAAGACGACCATCATTGA ATGTCTAAAGTATATTTGTACTGGAGATTTCCCTCCTGGAACCAAAGGAAATACATTTGTTCATGATCCC AAGGTTGCTCAAGAAACAGATGTGCGTGCCCAAATTCGCCTGCAGTTTCGTGATGTCAATGGAGAGATGG TACTTGTGCAGAGGTCCATGCTTTGCAGTCAGAAAAGTAAAAAAACCGAATTTAAAACCCTGGAAGGAGT CATTACTAGAATAAAGCACGGTGAAAAAGTCAGTCTCAGCTCCAAATGTGCAGAAATCGACCGAGAAATG ATAAGTTGTCTTGGGGTTTCCAAGTCTGTGCTAAACAATGTTATTTTCTGCCACCAAGAAGACTCAAATT GGCCTCTAAGTGAAGGAAAGGCTCTGAAGCAGAAATTTGATGAGATTTTTTCAGCAACAAGGTACATTAA AGCCCTAGATACGCTTCGACAGGTACGACAGACACAAGGTCAGAAAGTAAAAGAATGTCAAACAGAATTG AAATATCTGAGGCAAAATAAGGAGAAAGCTTGTGAGATCCGAGATCAGATCACTAGTAAGGAAGCCCAGT TAGCATCTTCACGGGAAATTGTCAAAGCCTATGAGAATGAGCTTGAGCCATTGAAGAATCGCCTGAAAGA GATTGAACATAACCTCTCTAAAATAATGAGACTTGACAATGAAATTAAAGCCTTGGATAGCAGAAAGAAG CAAATGGAAAAAGATAACAGTGAATTAGAACAGAAGATGGAAAAGGTTTTTCAAGGGACTGATGAGCAGC TAAATGACTTGTATCACAATCACCAGAGAACTGTAAGGGAGAAAGAAAGGCGCTTGGTAGACTGTCAGCG TGAACTGGAGAAGCTGAGTAAAGAAGCTCGGCTCCTCAACCAGGAAAGAGCAGAGCTGCTTGTGGAGCAG GGTCGTCTACAACTACAGGCAGATCGACATCAAGAGCATATCCGAGCCAGAGACTCACTGATTCAGTCTT TGGCAGCACATCTTGAATTGGATGGTTTTGAGCGTGGACCATTCAGTGAAAGACAGATTAAAAACTTTCA TGAACTTGTGAGAGAGAGACAGGAGAGAGAAGCTAAAACTGCCAGCCAGCTCTTGAGCGACCTTACAGAC AAAGAAGCGCTGAAGCAGAGACAGATGGATGAGATGAGGGACAAGAAGAGCGGGCTGGGGAGGATGATTG AGCTGAAGACCGAGATCCTGACGAAGAAGCAGACTGAGCTGAGGAACGTGAGGAATGAGCTGCAGCAGCT GGAGGGCTCCTCGGACAGGATTCTGGAGCTGGACCAGGAGCTCACAAAAGCGGAACGTGAACTAAGCAAG GCTGAGAAAAATAGCAGCATAGAAACCCTAAAAGCAGAAATACTAAACCTCCAAAGTGAGAAAGCGGACC TGGACAGGAACCTGCGGAAACTGGATCAGGAGATGGAGCAGTTAAACCATCATACAACAACCCGCACACA GATGGAGATGCTTACCAAAGACAAAACTGACAAAGATGAACAGATCAGAAAAATAAAGTCCAGGCACAGT GATGAACTAACTTCACTGTTGGGATATTTTCCTAACAAAAAACAGCTTGAAGACTGGCTTCATTCTAAAT CCAAAGAGATTAATCAGACCAGGGACAGACTTGCCAAACTGAACAAAGAACTAGCTTCAGCCGAACAAAA TAAAAATCATATAAATAATGAGCTAAAGAAAAAGGAAGAGCAGCTGTCTAGTTATGAAGATAAACTGTTT GATGTTTGTGGTAGCCAAGATTTTGAAAGTGACTTAGACAGACTTAAAGAAGATATTGAAAAATCCTCAA AGCAGCGAGCCATGCTGGCTGGAGCCACAGCAGTTTACTCCCAGTTCATCACTCAGCTGACAGATGAAAA CCAGTCCTGTTGCCCAGGCTGTCAGAGAGTATTTCAGACGGAAGCTGAATTACAGGAAGTCATCAGTGAC TTGCAGTCCAAGCTGAGGCTTGCTCCAGATAAACTCAAGTCAACAGAATCAGAACTAAAAAAAAAAGAGC GGCGCCGTGATGAAATGCTGGGGCTTGTGCCCATGAGGCAAAGCATAATTGATTTGAAGGAAAAGGAAAT ACCAGAATTAAGAAACAGACTGCAGAGTGTCAATAGAGACATACAGCGCCTAAAGAATGACATAGAGGAG CAGGAGACACTCTTGGGTACAGTGATGCCCGAAGAGGAAAGTGCTAAAGTGTGCCTGACAGACGTCACGA TCATGGAGAGGTTCCAGATGGAGCTAAAAGACGTTGAAAGGAAAATTGCACAGCAGGCAGCTAAGCTGCA GGGGGTAGACTTGGATCGGACTGTCCAGCAGGTTAACCAGGAAAAACAAGAAAAACAACACAAACTGGAT ACAGTTTCCAGTAAGATTGAATTGAACCGTAAGCTTATACAGGACCAGCAGGAACAAATTCAGCACCTGA AAAGTAAAACAAATGAGCTGAAATCAGAGAAACTGCAGATAGCCACCAATTTGCAACGGCGTCAGCAAAT GGAGGAGCAGACTGTGGAATTATCCACTGAAGTTCAGTCTTTGAACAGAGAAATAAAGGATGCTAAAGAG CAAATAAACCCTTTGGAGATAGCACTGGAAAAGTTGCAGCAGGAAAAAGAAGAATTGATCCACAGAAAAA ATACAAGTAACAAAATGGCTCAGGATAAGATCAATGATATCAAAGAGAAAGTTAAAAATATTCATGGTTA CATGAAAGACATAGAGAATTATATTCAAGATGGAAAAGATGACTATAAGAAGCAAAAAGAAACTGAACTT AATGAAGTTGTCATTCAACTAAATGAATGTGACAAACACAAAGAAAAGATAAATAAAGAAATGGGAACCA TGAGGCAAGATATTGACACGAAGAAGATACAGGAAAGGTGGTTACAGGATAACCTTACACTGAGAAAAAG AAGAGAGGAACTAAAAGAAGTTGAAGAGGAACGAAAGCAACATTTGAAGGAGATGGGACAGATGCAAGTT TTACAGATGAAAAATGAGCACCAGAAGTTGGAAGAGAACATAGACACGATAAAGAGAAATCACAGTTTGG CATTGGGGCGACAGAAAGGCTATGAAGAAGAAATTCTTCACTTTAAGAAGGAGCTCCGAGAACCTCAGTT TCGGGATGCAGAGGAGAAGTACAGAGAAATGATGATTGTCATGAGAACCACAGAGCTGGTGAATAAGGAC CTGGACATCTACTACAAAACTCTTGATCACGCAATAATGAAATTTCACAGTATGAAAATGGAAGAAATCA ACAAAATTATTCGTGATCTTTGGCGGAGTACCTATCGTGGGCAAGATATTGAATACATAGAAATTCGATC CGATGCTGATGAAAATGTATCAGCTTCTGATAAAAGGCGAAATTACAACTACCGAGTGGTGATGCTGAAG GGGGATACAGCCTTGGACATGCGCGGACGATGCAGTGCTGGACAGAAGGTGCTGGCCTCTCTCATCATCC GACTGGCCCTGGCCGAAACCTTCTGTCTGAACTGCGGCATCCTTGCCTTGGATGAGCCTACAACAAACCT GGACCGAGAAAACATCGAGTCTCTGGCACATGCTTTGGTTGAGATTATAAAAAGTCGCTCGCAGCAGCGC AACTTCCAGCTTCTGGTCATCACTCACGATGAAGATTTTGTGGAGCTCCTAGGACGATCTGAGTATGTGG AGAAATTCTACAGAGTGAAGAAGAACATCGACCAGTGCTCAGAGATTGTCAAGAGCAGCATCAACTCTCT GGGCTCTTATGTTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201717 representing NM_022246 Red=Cloning site Green=Tags(s) MSRIEKMSTLGVRSFGIEDKDKQIISFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDP KVAQETDVRAQIRLQFRDVNGEMVLVQRSMLCSQKSKKTEFKTLEGVITRIKHGEKVSLSSKCAEIDREM ISCLGVSKSVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALDTLRQVRQTQGQKVKECQTEL KYLRQNKEKACEIRDQITSKEAQLASSREIVKAYENELEPLKNRLKEIEHNLSKIMRLDNEIKALDSRKK QMEKDNSELEQKMEKVFQGTDEQLNDLYHNHQRTVREKERRLVDCQRELEKLSKEARLLNQERAELLVEQ GRLQLQADRHQEHIRARDSLIQSLAAHLELDGFERGPFSERQIKNFHELVRERQEREAKTASQLLSDLTD KEALKQRQMDEMRDKKSGLGRMIELKTEILTKKQTELRNVRNELQQLEGSSDRILELDQELTKAERELSK AEKNSSIETLKAEILNLQSEKADLDRNLRKLDQEMEQLNHHTTTRTQMEMLTKDKTDKDEQIRKIKSRHS DELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASAEQNKNHINNELKKKEEQLSSYEDKLF DVCGSQDFESDLDRLKEDIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPGCQRVFQTEAELQEVISD LQSKLRLAPDKLKSTESELKKKERRRDEMLGLVPMRQSIIDLKEKEIPELRNRLQSVNRDIQRLKNDIEE QETLLGTVMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGVDLDRTVQQVNQEKQEKQHKLD TVSSKIELNRKLIQDQQEQIQHLKSKTNELKSEKLQIATNLQRRQQMEEQTVELSTEVQSLNREIKDAKE QINPLEIALEKLQQEKEELIHRKNTSNKMAQDKINDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETEL NEVVIQLNECDKHKEKINKEMGTMRQDIDTKKIQERWLQDNLTLRKRREELKEVEEERKQHLKEMGQMQV LQMKNEHQKLEENIDTIKRNHSLALGRQKGYEEEILHFKKELREPQFRDAEEKYREMMIVMRTTELVNKD LDIYYKTLDHAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLK GDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSQQR NFQLLVITHDEDFVELLGRSEYVEKFYRVKKNIDQCSEIVKSSINSLGSYVH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022246
ORF Size	3936 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022246.1, NP_071582.1
RefSeq Size	4444 bp
RefSeq ORF	3939 bp
Locus ID	64012
UniProt ID	Q9JIL8
MW	153.8 kDa
Gene Summary	involved in DNA double-strand break repair [RGD, Feb 2006]

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