Alcam (NM_031753) Rat Untagged Clone
CAT#: RN201706
Alcam (untagged ORF) - Rat activated leukocyte cell adhesion molecule (Alcam), (10 ug)
CNY 7,410.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN201706 representing NM_031753
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTAAAGGGTCCCCTTCTTGCCGTCTGGTTTTCTGCCTCTTGATCTCCGCCGCGGTCCTCAGAC CAGGCCTCGGATGGTACACTGTCAACTCAGCATACGGAGATACCATTGTCATGCCTTGCAGACTGGATGT ACCTCAGAACCTCATGTTTGGCAAATGGAAATATGAAAAGCCTGATGGGTCCCCAGTATTTATTGCATTC AGATCTTCTACAAAGAAAAGTGTGCAGTATGATGATGTACCAGAGTACAAGGACAGACTGAGCCTCTCAG AAAACTACACTCTGTCTATCAACAATGCAAAGATCAGTGACGAAAAGAGATTTGTGTGCATGCTAGTGAC CGAGGACAACGTGTTTGAGGCACCTACCCTGGTCAAGGTGTTCAAGCAACCATCTAAACCTGAAATTGTA AACAGAGCAGCGTTTCTTGAAACAGAGCAGCTAAAAAAGTTAGGTGACTGCATTTCAAGAGACAGTTACC CAGACGGCAACATCACGTGGTATAGGAATGGGAAAGTGCTACAGCCTGTTGATGGAGAGGTGTCCATACT TTTTAAAAAGGAAATTGATCCAGGTACTCAGTTGTATACCATGACTTCCTCCTTGGAGTACAAGACAACC AAGTCTGACATACAAATGCCATTCACCTGTTCTGTGACATATTATGGACCTTCGGGCCAGAAAACAATTT ATTCTGAACAAGCAATCTTTGATATTTACTATCCTACAGAGCAGGTGACAATACAAGTACTGCCACCAAA AAATGCCATCAAAGAAGGGGACAACATCACCCTTCAGTGCTTGGGGAATGGCAACCCACCTCCTGAGGAG TTCATGTTTTACTTACCAGGGCAGGCTGAAGGCATAAGAAGCTCAAACACTTACACACTGACAGACGTGA GACGCAATGCCACCGGGGACTACAAATGTTCTCTCATCGACCAAAGAAACATGGCAGCTTCAACAACCAT CACTGTTCACTACTTGGATTTATCCTTAAACCCAAGTGGGGAAGTGACCAAGCAGATCGGTGATACCCTG CCTGTGTCTTGCACAATATCTGCAAGTAGGAATGCAACTGTGGTGTGGATGAAGGATAACATCAGGCTCC GATCTAGTCCATCCTTTTCTAGTCTTCATTATCAGGATGCTGGGAACTATGTCTGTGAAACTGCTCTTCA GGAGGTTGAGGGACTGAAGAAAAGGGAGTCGCTGACCCTCATCGTAGAAGGAAAACCTCAAATCAAAATG ACAAAGAAAACTGATCCCAGTGGACTGTCTAAGACTATAATCTGCCATGTGGAAGGGTTTCCAAAGCCAG CTATACAGTGGACCATTACCGGCAGTGGAAGCGTCATAAACCAAACAGAGGAGTCTCCTTATATTAATGG CAGGTATTATAGTAAAATTATCATTTCCCCTGAGGAGAATGTTACATTAACTTGCACAGCAGAAAACCAA CTGGAGAGAACAGTAAACTCCCTGAATGTCTCTGCGATAAGTATTCCAGAACACGATGAGGCAGACGATA TAAGTGATGAAAACAGAGAAAAGGTGAATGACCAGGCCAAACTAATTGTGGGCATTGTGGTTGGTCTCCT CCTCGCCGCCCTCGTCGCCGGTGTCGTCTACTGGCTGTACATGAAGAAATCGAAAACTGCATCAAAACAT GTAAACAAGGACCTTGGTAATATGGAGGAGAATAAAAAGCTAGAAGAAAACAATCACAAAACAGAAGCCT AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_031753 |
Insert Size | 1752 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031753.1, NP_113941.1 |
RefSeq Size | 2866 bp |
RefSeq ORF | 1752 bp |
Locus ID | 79559 |
UniProt ID | O35112 |
Gene Summary | Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR201706 | Alcam (Myc-DDK-tagged ORF) - Rat activated leukocyte cell adhesion molecule (Alcam), (10 ug) |
CNY 7,410.00 |
|
RR201706L3 | Lenti ORF clone of Alcam (Myc-DDK-tagged ORF) - Rat activated leukocyte cell adhesion molecule (Alcam), (10 ug) |
CNY 9,410.00 |
|
RR201706L4 | Lenti ORF clone of Alcam (mGFP-tagged ORF) - Rat activated leukocyte cell adhesion molecule (Alcam), (10 ug) |
CNY 10,260.00 |