Nes (NM_012987) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201694 representing NM_012987 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTTGCGTCGGGGAAGAATCTTTTCAGATGTGGGAGCTCAATCGACGCCTGGAGGCCTACCTGA CCCGGGTCAAGACGCTAGAAGAGCAAAACCAGCTGCTCAGCGCCGAGCTTGGGGGACTCCGGGCGCAGTC CGGAGACACCTCCTGGAGAGCCCGAGCCGATGACGAGCTGGCATCCCTGCGGATCCTCGTCGATCAGCGC TGGCGGGAGAAGCTCGAGGCTGAGGTGCAGCGCGACAACCTTGCGGAAGAGCTGGAGAGCGTGGCGGGCC GGTGCCAGCAGGTGCGGCTTGCTCGGGAGCGGACCGTCCAGGAGGCCGCCTGCAGCCGGCGCGCACTCGA GGCGGAGAAGAATGCGCGGGGCTGGCTGAGCACCCAGGCGGCCGAGCTGGAGCGGGAGTTAGAGGCTCTG CGAGCCGCGCACGAGGAGGAGCGCGCACACCTGAACGCCCAGGCCGCCTGTGCGCCTCGCCGGCCCCCCG CACCGCCCCACGGATCCCCGGTCCGGGCCCCCGAAGTCGAGGATCTGGCCAGGCGACTAGGCGAAGTGTG GCGCGGGGCGGTGCGTGACTACCAGGAGCGCGTGGCTCACATGGAAAGCTCGCTGGGTCAGGCACGCGAG CGGCTGAGCCAAGCCGTGCGGGGCGCTCGGGAGTGTCGCTTAGAGGTGCAACAGCTGCAGGCTGATCGCG ACAGCCTCCAGGAGCGCAGAGAAGCGCTGGAACAGAGATTGGAAGGCCGCTGGCAGGACCGGCTGCAGGC CACTGATAAGTTCCAGCTGGCTGTGGAAGCCCTGGAGCAGGAGAAGCAAGGTCTACAGAGTCAGATCGCT CAGATCCTGGAAGGTGGGCAGCAACTGGCACACCTCAAGATGTCCCTTAGTCTGGAGGTGGCTACATACA GGACTCTGCTGGAGGCTGAGAACTCTCGGTTGCAGACACCTGGACGAGGTTCCCAGGCTTCTCTTGGCTT TCTGGACCCCAAGCTGAAGCCGAATTTCCTTGGGATACCAGAGGACCAGTACCTGGGATCTGTGCTCCCT GCCCTCAGCCCCACATCCTTCCCTTCCCCCTTGCCTAATACCCTTGAGACTCCTGTGACAGCCTTCCTGA AGACTCAGGAGTTCCTTCAGGCCAGAACCCCCACCTTGGCCAGCACTCCCATCCCACCTATATCTGAGGC TCCCTGTCCTCCAAATGCAGAGGTCAGAGCCCAGGAGGTCCCTCTTTCTCTGCTCCAGACACAGGCTCCA GAGCCCCTTTGGGCTGAGGCCACAGTGCCTAGTTCTTCTGCTATCCTCCCAGAACTAGAGGAACCTGGGG GCAAGCAGCAGGGTCACTTCCCTGATGATCTGACCTCCTTAGCCACAACCCTCAACCCTCACCACCCTAC TTTAGAGGCTAAAGATGGAGAATCCAGTGAGTCTAGAGTTTCTAGCATATTCCAGGAAGATGAGGGGCAA ATCTGGGAACTGGTAGAGAAAGAAGCAGATATAGAGGTAAAAGTAGAAAACAGCTCAGCCCAGAAAACAC AAGAAAGTGGTCTGGACACAGAAGAAACCCAGGATTCCCAGGGACCTTTGCAGAAGGAAACACTGAAGGC TCTAGGAGAGGAGCCACTGATGTCTCTGAAAATCCAGAACTATGAGACAGCAGGGAAAGAGAATTGCAAT TCTTCTACAGAAGGCCACCTGGGAACACTAGAAGGCCCAGAAAAAGAAAAGCAAATACCACTAAAGTCTT TAGAAGAAAAGAATGTAGAGTCAGAGAAAACTCTAGAAAATGGGGTTCCTGTACTATCTGAGCTTTTAGG AAAAGAAGACACAAGAACAGAGGATCAAGAATTAATGTCTCCTAAAGGTACACTAAAGAGATTTTCATCT CTAGGAAAGGAAAGTCAAGAAGTAGTGAGGCCTTCAAAAGAGGGGAACCTAGAATCATGGACAGCTTTTA AAGAGGAGAGCCAACACCCACTGGGATTTCCAGGAGCTGAGGACCAGATGCTTGAGAGACTGGTAGAGAA AGAGGATCAGAGCTTCCCAAGGTCTCCAGAGGAAGAGGACCAGGAGGCATGTAGACCTCTGCAGAAAGAG AATCAGGAACCACTAGGGTATGAAGAAGCAGAGGGCCAGATACTTGAGAGACTGATAGAAAAAGAGAGTC AGGAGTCCCTGAGGTCTCCAGAAGAAGAGGACCAGGAGGCAGGTAGATCTCTGCAGAAAGAGAATCAGGA GCCACTAGGGTATGAAGAAGCAGAGGACCAGATGCTTGAGAGACTGATAGAAAAAGAGAGTCAGGAGTCC CTGAAGTCTCCAGAAGAAAACCAGAGGATTGGGAAGCCTCTAGAAAGAGAGAATCAGAAATCTCTGAGGT ATCTTGAAGAAAACCAGGAGACTTTTGTACCACTAGAAAGCAGGAACCAGAGGCCACTGAGATCTCTAGA AGTAGAAGAGGAGGAGCAGAGAATTGTGAAACCTCTAGAAAAAGTGAGTCAGGATTCCCTCGGATCTCTA GCAGAAGAGAATGTGCAGCCACTGAGGTATCTGGAAGAAGATAACTGCATAAATAAGAGCCTTCTAGAAG ACAAGACTCACAAGTCCTTGGGGTCTCTTGAAGATAGAAATGGGGATAGCATTATTATACCACAAGAAAG TGAGACCCAGGTTTCATTGAGGCCTCCAGAAGAGGAGGACCAGAGGATTGTGAACCATCTAGAAAAAGAA AGTCAGGAGTTCTCAAGGTCTTCAGAAGAAGAAGAGCAGGTGATGGAGAGATCTCTAGAAGGAGAGAACC ATGAATCACTGAGTTCTGTAGAAAAAGAGGACCAGATGGTTGAGAGCCAACTAGAGAAAGAGAGTCAGGA CTCAGGGAAGTCTCTTGAAGATGAGAGCCAGGAGACTTTTGGACCTCTGGAAAAAGAGAATGCAGAGTCC CTGAGATCTCTAGCAGGACAGGACCAAGAGGAACAGAAGCTTGAACAAGAGACCCAACAAACACTGAGGG CTGTAGGGAATGAGCAGATGGCAGTGAGCCCACCAGAAAAGGTGGATCCAGAGTTACCGAAGCCTCTTGG AAATGACCAGGAAATAGCTAGATCTCTTGGAAAAGAGAATCAAGAGTCACTAGTGTCACTGAAAGAAAAA GGTATAGAGACAGTGAAGTCTTTAGAAACAGAGATCATAGAACCACTGGAGACTGCAGAAGAGGACCTGG AAAGAAGGAAGTCTATAGATACTCAGGAGCCATTGTGGTCTACTGAAGTGGCTAGAGAGACAGTAGAACC TCCAGAAGATGAGCCCCCAGGATCGCTAGGGTCTGTGGATGAGAACCGAGAGACACTGACATCCCTTGAA AAGGAGAGTCAAGAACTGAGCTCTCTGGGCAAGTGGAACGTAGAGACCAGGGTAGAGGACAGTCAGCAGT GCCTGCAAGTAGAAGAGGGTCTGCAGGAGGAACAGCACCAAGAGTCTCTGAGAGAGGTGAAGCAGGAGCT GCCTAGCTCTGGAAATCAACAGCGGTGGGAGGATGTGGTGGAGGGCAAAGCAGTGGGTCAGGAAGCACCT CTGGCAACCACAGGAGTGGGAACTGAGGATAAGGCAGAGTTGCATCTGAGGGGGCAAGGTGGAGAGGAAG AAGCTGCAGCAGAGGGAGAGCTGTTGCAGGATATTGTGGGGGAGGCCTGGAGTCTGGGGAGCTCTGAGCC CAAGGAGCAGAGGGTCCCTGCTGAGGCCCTCGACAACCTGGAAGGAGGGGCCTTAGAGGTCCCAGTTGCT CAGTCAATGCCAGAGGTGACAGAGCGAGATGAGGATAGAGCCCAAGCAGGTGAACAAGACTCCATAGAGG TGACCCTTGGGTTAGAGGCTGCCAGAACTGGACTGGAACTCGAGCAGGAAGTGGTAGGGCTAGAGGACCC AAGGCATTTTGCCAGGGAGGAGGCCATTCCCCCATCCCTGGGGGAGGAAAGTGTGAAGGCAAAGATAGCT CAGGGCTTGGAAGGGCCTGGAAAGGAACCAAAAGAGGCAGGTGCTCTGGACTCGGGGATCCTTGAATTGC CCAAGACTAGCAGCGAGGCTCTGGAATGCCAGGGCCATGAAGAGTCTGAGTCCATGGAGGGCTGGGAAGA AGAGGAGGCCTCACTGGAGACTTCAGATCATGAGGGCAGTGATGCCCCTCAGCCCAGGCCCCCAGAAACA GAAGAAGATGAGGGTGCACAGGCAGCACTGACAGCCCCTGGTCCCAAGCTCTTGGAACCCTGTTCACCCA TCCCAATCCTGACAGATGCCCATGAGCTGCAGCCCCAGGCTGAGGGGATCCAGGAGGCTGGCTGGCAGCC AGAAGCTGGGTCTGAAGCACTAGAAAGGGTAGAAAATGAGCCAGAGTTTGGTCTTGGGGAGATCCCGGAG GGCCTCCAGGATTGGGAAGAGGGCAGAGAAGAAAGCGAGGCAGATGATCTAGGGGAAACTCTCCCTGACT CTACTCCCCTGGGCCTCTACCTGAGGTCCCCTGCTTCTCCAAAGTGGGATCTGGCTGGAGAACAGAGGCT TTCCCCTCAAGGGGATGCCGGGAAGGAAGACTGGGGTCCTGCTGTCCCCGCTGCCCAGGGCCTCAGTGGT CCACCGGAAGAGGAGGAGGAGCAAGGCCATGGCTCTGACCTATCATCTGAAGAGTTTGAGGACCTAGGGA CTGAGGCCTCTCTTCTTCCAGGGGTTCCCAAGGAGGTAGCAGATCACGTGGGCCAAGTGCCCCCGGTACT GCAGCCTGCATGCTGGGATCAGGGTGGGGAATCTGATGGGTTTGCTGATGAGGAAGAAAGTGGGGAGGAG GGAGAGGAAGAAGATGCTGATGAGGAAGGAGCAGAGTCAGGAGCTCAGTGGTGGGGGTCAGGGGCCTCTG GTGGAGGCTGCAAGGTCCAGGATATTGCCCAAAGAGGAGACCCGGTACAGGAGTCTGTGGGTGTCAGTGG TCTCTGGGATGATGGCTTGAGAGGTGCTGCAGCTAATGTTCCTGCCCTAGAGATGGTATCTCAGGACAGT GCTGAGCCTTCTGGGTCAGAGGAGTCTGAGTCTGCTTCCTTGGAGGGGGAGGAAGGTCAAGTGACTGACC ATTTAGATGCTCCCCAGGAGGTGACCAGCATGGTCCCGGGGGTAGGAGATGCCTTTGACATTGGTGGCCA GAGCCCCAACTTGGACTCAGAACAAGTGAATGGGAAAATGGAGAATGGACTAGAACAGGCTGAGGGGCAG GTGGTCCTGGATGGGGACGAGGATCAAGAACTCCTATTACAGGGACAGGAGGTGGGTGCTCTAAAGGTTC CTTTGGTAGCATCTCCTGTGCATCTAGGCCCAAGCCAGCCCCTGAAGTTCACTCTGAGTGGGGTAGATGG GGATTCCTGGTCCTCAGGGGAAGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012987
Insert Size	5418 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012987.2, NP_037119.2
RefSeq Size	5949 bp
RefSeq ORF	5418 bp
Locus ID	25491
Gene Summary	intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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